Data

Viral abundance and productivity in the Southern Ocean - data collected from the BROKE-West voyage of the Aurora Australis, 2006

Australian Antarctic Division
Ashcroft, D., Mitchell, J. and Seuront, L. ; ASHCROFT, DANIEL ; MITCHELL, JAMES ; SEURONT, LAURENT
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/15/59927327626e7&rft.title=Viral abundance and productivity in the Southern Ocean - data collected from the BROKE-West voyage of the Aurora Australis, 2006&rft.identifier=10.4225/15/59927327626e7&rft.publisher=Australian Antarctic Division&rft.description=Data acquisitionSamples were collected at the 3 sites at 9 different depths.Depths included the bottom depth as well as 8 samples in the top 200M.50ml samples were collected from the niskin bottles attached to the CTD.20ml was filtered through a 0.02 microlitre filter to remove the viruses and larger organisms from the sample.1ml of this virus free water was then pipetted into individual cryovials.A further 1ml was then taken from the original seawater sample and was then passed through a 0.2 microlitre filter, which retained all the bacteria and algae.The 0.2 microlitre filter was then placed in the cryovial with the 1ml of virus free seawater.Samples were then fixed with glutaraldehyde (5 microlitre, 50% concentration) at hourly intervals for 4 hours.After fixation samples were placed in liquid nitrogen and later stored at -80 degrees C.All samples will be analysed using a flowcytometer.The fields in these datasets are:DateLeg numberLatitudeLongitudeSubsampleDepth (m)Bottle NumberViscosity (cP)This work was completed as part of ASAC projects 2655 and 2679 (ASAC_2655, ASAC_2679).Progress Code: completedStatement: Samples were taken at 3 stations along leg 1. 9 depths were sampled at the 3 sites and depths were chosen based on the fluorescence maximum on the downcast of the CTD.&rft.creator=Ashcroft, D., Mitchell, J. and Seuront, L. &rft.creator=ASHCROFT, DANIEL &rft.creator=MITCHELL, JAMES &rft.creator=SEURONT, LAURENT &rft.date=2006&rft.coverage=westlimit=30.0; southlimit=-69.04; eastlimit=30.0; northlimit=-61.0&rft.coverage=westlimit=30.0; southlimit=-69.04; eastlimit=30.0; northlimit=-61.0&rft_rights=These data are publicly available from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_viralabundance when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON&rft_subject=viral abundance&rft_subject=Viral productivity&rft_subject=southern ocean&rft_subject=Date&rft_subject=Leg number&rft_subject=Latitude&rft_subject=Longitude&rft_subject=Subsample&rft_subject=Depth&rft_subject=Bottle Number&rft_subject=Viscosity&rft_subject=CTD > Conductivity, Temperature, Depth&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=ACE/CRC&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_viralabundance when using these data.

This metadata record is publicly available.

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Full description

Data acquisition

Samples were collected at the 3 sites at 9 different depths.
Depths included the bottom depth as well as 8 samples in the top 200M.
50ml samples were collected from the niskin bottles attached to the CTD.
20ml was filtered through a 0.02 microlitre filter to remove the viruses and larger organisms from the sample.
1ml of this virus free water was then pipetted into individual cryovials.
A further 1ml was then taken from the original seawater sample and was then passed through a 0.2 microlitre filter, which retained all the bacteria and algae.
The 0.2 microlitre filter was then placed in the cryovial with the 1ml of virus free seawater.
Samples were then fixed with glutaraldehyde (5 microlitre, 50% concentration) at hourly intervals for 4 hours.
After fixation samples were placed in liquid nitrogen and later stored at -80 degrees C.
All samples will be analysed using a flowcytometer.

The fields in these datasets are:

Date
Leg number
Latitude
Longitude
Subsample
Depth (m)
Bottle Number
Viscosity (cP)

This work was completed as part of ASAC projects 2655 and 2679 (ASAC_2655, ASAC_2679).

Lineage

Progress Code: completed
Statement: Samples were taken at 3 stations along leg 1. 9 depths were sampled at the 3 sites and depths were chosen based on the fluorescence maximum on the downcast of the CTD.

Data time period: 2006-01-10 to 2006-02-28

This dataset is part of a larger collection

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30,-69.04 30,-61

30,-65.02

text: westlimit=30.0; southlimit=-69.04; eastlimit=30.0; northlimit=-61.0

Subjects
ACE/CRC | AMD | AMD/AU | Bottle Number | CEOS | CONTINENT > ANTARCTICA | CTD > Conductivity, Temperature, Depth | Date | Depth | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON | GEOGRAPHIC REGION > POLAR | Latitude | Leg number | Longitude | OCEAN > SOUTHERN OCEAN | R/V AA > R/V Aurora Australis | SHIPS | Subsample | Viral productivity | Viscosity | biota | oceans | southern ocean | viral abundance |

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Other Information
Download point for the data - csv files (GET DATA)

local : data.aad.gov.au/eds/3262/download

Download point for the data - CTD Bottle Water Data Index page (GET DATA)

url : http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm

Public information for ASAC project 2655 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=2655

Public information for ASAC project 2679 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=2679

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_viralabundance

Identifiers
ACN 633 798 857