Data

Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018.

Australian Antarctic Data Centre
MCINNES, JULIE C. ; SHAW, JUSTINE
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5bkj-yg59&rft.title=Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018.&rft.identifier=10.26179/5bkj-yg59&rft.publisher=Australian Antarctic Data Centre&rft.description=Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.&rft.creator=MCINNES, JULIE C. &rft.creator=SHAW, JUSTINE &rft.date=2021&rft.coverage=northlimit=-54.46685; southlimit=-54.79277; westlimit=158.74146; eastLimit=158.98865; projection=WGS84&rft.coverage=northlimit=-54.46685; southlimit=-54.79277; westlimit=158.74146; eastLimit=158.98865; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.&rft_subject=biota&rft_subject=ALBATROSSES/PETRELS AND ALLIES&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=ANIMALS/VERTEBRATES&rft_subject=BIRDS&rft_subject=EXOTIC SPECIES&rft_subject=BIOSPHERE&rft_subject=ECOLOGICAL DYNAMICS&rft_subject=SPECIES/POPULATION INTERACTIONS&rft_subject=INDIGENOUS/NATIVE SPECIES&rft_subject=POPULATION DYNAMICS&rft_subject=MISEQ&rft_subject=LC480&rft_subject=AAD GENTICS LAB&rft_subject=DNA METABARCODING&rft_subject=PROCELLARIIDAE&rft_subject=PELECANOIDES&rft_subject=RECOLONISATION&rft_subject=BURROWING PETRELS&rft_subject=ADS > Automated DNA Sequencer&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.

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Brief description

Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.

Issued: 2021-04-20

Data time period: 2017-11-09 to 2018-11-30

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158.98865,-54.46685 158.98865,-54.79277 158.74146,-54.79277 158.74146,-54.46685 158.98865,-54.46685

158.865055,-54.62981

text: northlimit=-54.46685; southlimit=-54.79277; westlimit=158.74146; eastLimit=158.98865; projection=WGS84

Subjects
AAD GENTICS LAB | ADS > Automated DNA Sequencer | ALBATROSSES/PETRELS AND ALLIES | ANIMALS/VERTEBRATES | BIOLOGICAL CLASSIFICATION | BIOSPHERE | BIRDS | BURROWING PETRELS | DNA METABARCODING | EARTH SCIENCE | ECOLOGICAL DYNAMICS | EXOTIC SPECIES | FIELD SURVEYS | GEOGRAPHIC REGION > POLAR | INDIGENOUS/NATIVE SPECIES | LABORATORY | LC480 | MISEQ | OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND | PELECANOIDES | POPULATION DYNAMICS | PROCELLARIIDAE | RECOLONISATION | SPECIES/POPULATION INTERACTIONS | biota |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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