Data

Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018.

Australian Antarctic Division
Mcinnes, J.C. and Shaw, J. ; MCINNES, JULIE C. ; SHAW, JUSTINE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5bkj-yg59&rft.title=Using DNA metabarcoding to detect burrowing seabirds in a remote landscape, Macquarie Island 2017-2018.&rft.identifier=10.26179/5bkj-yg59&rft.publisher=Australian Antarctic Data Centre&rft.description=Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.Progress Code: completedStatement: See the methodology document in the download file for more information.&rft.creator=Mcinnes, J.C. and Shaw, J. &rft.creator=MCINNES, JULIE C. &rft.creator=SHAW, JUSTINE &rft.date=2021&rft.coverage=westlimit=158.74146; southlimit=-54.79277; eastlimit=158.98865; northlimit=-54.46685&rft.coverage=westlimit=158.74146; southlimit=-54.79277; eastlimit=158.98865; northlimit=-54.46685&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > EXOTIC SPECIES&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > INDIGENOUS/NATIVE SPECIES&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > POPULATION DYNAMICS&rft_subject=MISEQ&rft_subject=LC480&rft_subject=AAD GENTICS LAB&rft_subject=DNA METABARCODING&rft_subject=PROCELLARIIDAE&rft_subject=PELECANOIDES&rft_subject=RECOLONISATION&rft_subject=BURROWING PETRELS&rft_subject=ADS > Automated DNA Sequencer&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=CEOS&rft_subject=AMD/AU&rft_subject=AMD&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
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These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds when using these data.

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Full description

Scat and discarded feather samples were collected from outside burrowing petrel burrows across Macquarie Island (222 scat and 108 feather samples) between November 2017 and November 2018. Samples were collected within seabird colonies during ground searches and GPS co-ordinates were taken for each sample collected. DNA was extracted from all samples and two short mitochondrial markers (COI and 16S) were amplified using custom-designed burrowing petrel primers. Amplicons were pooled and sequenced on an Ilumina Miseq and compared to reference databases to provide species-level identifications. To assign species, maximum likelihood trees were constructed using MEGA X with representative species sequences from field samples and reference sequences from the Barcode of Life Database.

Lineage

Progress Code: completed
Statement: See the methodology document in the download file for more information.

Notes

Purpose
We tested the efficacy of DNA analysis to identify burrowing petrel species in a rapidly changing landscape, on a remote sub-Antarctic island following pest eradication. Using these data, we identify species presence and mapped the locations where species were detected with DNA metabarcoding.

Data time period: 2017-11-09 to 2018-11-30

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158.98865,-54.46685 158.98865,-54.79277 158.74146,-54.79277 158.74146,-54.46685 158.98865,-54.46685

158.865055,-54.62981

text: westlimit=158.74146; southlimit=-54.79277; eastlimit=158.98865; northlimit=-54.46685

Subjects
AAD GENTICS LAB | ADS > Automated DNA Sequencer | AMD | AMD/AU | BURROWING PETRELS | CEOS | DNA METABARCODING | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > EXOTIC SPECIES | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > INDIGENOUS/NATIVE SPECIES | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > POPULATION DYNAMICS | FIELD SURVEYS | GEOGRAPHIC REGION > POLAR | LABORATORY | LC480 | MISEQ | OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND | PELECANOIDES | PROCELLARIIDAE | RECOLONISATION | biota |

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Other Information
Download the dataset. (GET DATA > DIRECT DOWNLOAD)

url : https://data.aad.gov.au/eds/5245/download

Public information for AAS project AAS_4305 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=AAS_4305

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

url : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4305_McInnes_et_al_2021_DNA_detection_seabirds

Identifiers
ACN 633 798 857