Data

Use of the Pacific oyster (Magallana gigas) as a natural sampler for the detection of Neoparamoeba perurans

Commonwealth Scientific and Industrial Research Organisation
Bolte, Brett ; Bissett, Andrew ; Craw, Pascal ; McDougall, Carmel ; Wynne, James
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/96h1-ra18&rft.title=Use of the Pacific oyster (Magallana gigas) as a natural sampler for the detection of Neoparamoeba perurans&rft.identifier=https://doi.org/10.25919/96h1-ra18&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=qPCR quantifications and calculations for experimental oysters for assessing their ability to accumulate DNA of Neoparamoeba perurans within four organs of interest (Mantle, Gill, Palp, Digestive gland). This data contains beta actin (SYBR) values that serving as an DNA input standard as well as Neoparamoeba perurans (TaqMan) 18S DNA quantities. Calculations for the Beta actin/N. perurans DNA ratio, total amoeba within a tissue and copies/reaction are included in the metadata. Lineage: qPCR data was produced using a QuantStudio5 machine and analysed using Design and Analysis 2.6.0 Real Time PCR System software. &rft.creator=Bolte, Brett &rft.creator=Bissett, Andrew &rft.creator=Craw, Pascal &rft.creator=McDougall, Carmel &rft.creator=Wynne, James &rft.date=2025&rft.edition=v1&rft_rights=Creative Commons Attribution 4.0 International Licence https://creativecommons.org/licenses/by/4.0/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2025.&rft_subject=AGD&rft_subject=natural samplers&rft_subject=eDNA&rft_subject=Environmental assessment and monitoring&rft_subject=Environmental management&rft_subject=ENVIRONMENTAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Data is accessible online and may be reused in accordance with licence conditions

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qPCR quantifications and calculations for experimental oysters for assessing their ability to accumulate DNA of Neoparamoeba perurans within four organs of interest (Mantle, Gill, Palp, Digestive gland). This data contains beta actin (SYBR) values that serving as an DNA input standard as well as Neoparamoeba perurans (TaqMan) 18S DNA quantities. Calculations for the Beta actin/N. perurans DNA ratio, total amoeba within a tissue and copies/reaction are included in the metadata.
Lineage: qPCR data was produced using a QuantStudio5 machine and analysed using Design and Analysis 2.6.0 Real Time PCR System software.

Available: 2025-08-01

Data time period: 2025-07-17 to ..

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Subjects
AGD | Environmental Sciences | Environmental Assessment and Monitoring | Environmental Management | eDNA | natural samplers |

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Other Information

Australian Research Council (ARC) : CRC-20180101

Identifiers
ACN 633 798 857