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Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus

Flinders University
James Dorey (Aggregated by) Mark Stevens (Aggregated by) Michael Gardner (Aggregated by) Michael Schwarz (Aggregated by) Olivia Davies (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25451/flinders.19193519.v1&rft.title=Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus&rft.identifier=10.25451/flinders.19193519.v1&rft.publisher=Flinders University&rft.description=Datasets associated with Davies et al. 2022 Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus, Current Research in Insect Science.We describe widespread and conserved mitochondrial heteroplasmy in Australian native bee, Amphylaeus morosus and the co-occurring co-infection of the bacterial endosymbiont, Wolbachia across the species' distribution.Amphylaeus_iontorrent_mtgenome.zip = compressed folder of the partial mitogenome alignment and raw data for a single Amphylaeus morosus individual with DNA sequence data generated using Ion Torrent shotgun sequencing.Amphylaeus_sangerseq_mt-coi-gene.zip = compressed folder of DNA chromatograms of the (heteroplasmic) mitochondrial COI gene of 70 Amphylaeus morosus individuals with DNA sequences generated using Sanger sequencing.Wolbachia_BEAST_phylog_log.zip = compressed folder of DNA alignment and BEAST2 phylogenetic log and output files for the phylogenetic analysis of the Wolbachia COI-like and wsp genes from host, Amphylaeus morosus.Wolbachia_cloning.zip = compressed folder of DNA chromatograms of the cloning products of two Amphylaeus morosus individuals for the two Wolbachia gene regions wsp and COI-like, generated using Sanger sequencing.Wolbachia_sangerseq_coi-like-gene.zip = compressed folder of DNA chromatograms of the Wolbachia COI-like gene region (with heterogeneious base pairs indicating a bacterial co-infection) of 27 Amphylaeus morosus individuals, with DNA sequences generated using Sanger sequencing.&rft.creator=James Dorey&rft.creator=Mark Stevens&rft.creator=Michael Gardner&rft.creator=Michael Schwarz&rft.creator=Olivia Davies&rft.creator=Tessa Bradford&rft.date=2022&rft_rights= https://creativecommons.org/licenses/by/4.0/&rft_subject=Evolutionary biology not elsewhere classified&rft_subject=Animal cell and molecular biology&rft_subject=Genetics not elsewhere classified&rft_subject=Invertebrate biology&rft_subject=Molecular evolution&rft_subject=Anthropological genetics&rft_subject=Behavioural genetics&rft_subject=Hymenoptera&rft_subject=maternal inheritance&rft_subject=endosymbiont&rft_subject=Hylaeinae&rft_subject=mitochondrial heterogeneity&rft_subject=Phylogenetic&rft_subject=Evolutionary Biology&rft_subject=Animal Cell and Molecular Biology&rft_subject=Genetics&rft_subject=Invertebrate Biology&rft_subject=Molecular Evolution&rft_subject=Population, Ecological and Evolutionary Genetics&rft.type=dataset&rft.language=English Access the data
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Datasets associated with Davies et al. 2022 "Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus", Current Research in Insect Science.

We describe widespread and conserved mitochondrial heteroplasmy in Australian native bee, Amphylaeus morosus and the co-occurring co-infection of the bacterial endosymbiont, Wolbachia across the species' distribution.

Amphylaeus_iontorrent_mtgenome.zip = compressed folder of the partial mitogenome alignment and raw data for a single Amphylaeus morosus individual with DNA sequence data generated using Ion Torrent shotgun sequencing.

Amphylaeus_sangerseq_mt-coi-gene.zip = compressed folder of DNA chromatograms of the (heteroplasmic) mitochondrial COI gene of 70 Amphylaeus morosus individuals with DNA sequences generated using Sanger sequencing.

Wolbachia_BEAST_phylog_log.zip = compressed folder of DNA alignment and BEAST2 phylogenetic log and output files for the phylogenetic analysis of the Wolbachia COI-like and wsp genes from host, Amphylaeus morosus.

Wolbachia_cloning.zip = compressed folder of DNA chromatograms of the cloning products of two Amphylaeus morosus individuals for the two Wolbachia gene regions wsp and COI-like, generated using Sanger sequencing.

Wolbachia_sangerseq_coi-like-gene.zip = compressed folder of DNA chromatograms of the Wolbachia COI-like gene region (with heterogeneious base pairs indicating a bacterial co-infection) of 27 Amphylaeus morosus individuals, with DNA sequences generated using Sanger sequencing.


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Subjects
Animal Cell and Molecular Biology | Animal cell and molecular biology | Anthropological genetics | Behavioural genetics | Evolutionary Biology | Evolutionary biology not elsewhere classified | Genetics | Genetics not elsewhere classified | Hylaeinae | Hymenoptera | Invertebrate Biology | Invertebrate biology | Molecular Evolution | Molecular evolution | Phylogenetic | Population, Ecological and Evolutionary Genetics | endosymbiont | maternal inheritance | mitochondrial heterogeneity |

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Identifiers
ACN 633 798 857