Data

Understanding the impact of change on Australia's World Heritage subantarctic islands - Anatomy studies of vascular plants Macquarie Island

Australian Antarctic Division

Bergstrom, D.M. and Ball, M. ; BERGSTROM, DANA M. ; BALL, MARILYN

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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/ASAC_3095_Anatomical_Data&rft.title=Understanding the impact of change on Australia's World Heritage subantarctic islands - Anatomy studies of vascular plants Macquarie Island&rft.identifier=http://data.aad.gov.au/metadata/ASAC_3095_Anatomical_Data&rft.publisher=Australian Antarctic Data Centre&rft.description=The dataset contains a series of anatomical images of plant specimens collected as part of AAS (ASAC) project 3095 at Macquarie Island during the summer of the 2011/2012 season.Species The four species examined were Azorella macquariensis, Colobanthus muscoides, Stilbocarpa polaris and Agrostis magellanica. Samples labelled 'South' were from Hurd point either near the hut (Colobanthus Stilbocarp and Agrostis) or from near the track at the top of the Hurd Point jump-up. The remaining plants were collected from the northern end of the island near the station.Anatomy and Leaf areaThe anatomy of the four species were investigated by taking hand cut sections and where necessary, staining with Toluidine Blue (0.05% in distilled water). Both transverse and longitudinal sections were taken. Photographic images were taken using Nikon Coolpic camera with eye tube adjustment. Images were accompanied with stage micrometre images. Two microscopes were used: a dissecting Leica MZ 8 and a Zeiss Axioscope. In some samples corresponding leaf surfaces were photographed of leaves removed from branches above the location of the transverse sections to calculate xylem surface area to leaf area ratios. Leaves were removed from the branches and places on graph paper under glass slides and photographed. Scales of the graph paper is 1 x 1 mm. Rehydration sequenceRehydration sequences of xylem vessels in both Colobanthus and Azorella samples were created by dissecting out xylem strands from longitudinal sections and drying on a glass slide. Water was then applied to rehydrate the cells and the sequence of embolism collapse was observed and photographed at defined time intervals. Interval measure had some non-measured margin of error as the shutter control on the camera was manually operated. Repeat drying and rehydration sequences were performed on the same sample. Two plants labelled Azorella macquariensis MC Ball experimental plant 1 and MC Ball Azorella no 2 Kleaf have associated physiological experimental data collected from them by Marilyn Ball.Progress Code: completed&rft.creator=Bergstrom, D.M. and Ball, M. &rft.creator=BERGSTROM, DANA M. &rft.creator=BALL, MARILYN &rft.date=2012&rft.coverage=westlimit=158.86; southlimit=-54.65; eastlimit=158.862; northlimit=-54.59&rft.coverage=westlimit=158.86; southlimit=-54.65; eastlimit=158.862; northlimit=-54.59&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_3095_Anatomical_Data when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > ANGIOSPERMS (FLOWERING PLANTS)&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > TERRESTRIAL ECOSYSTEMS > ISLANDS&rft_subject=Macquarie Island&rft_subject=Anatomy&rft_subject=Azorella macquariensis&rft_subject=Colobanthus muscoides&rft_subject=Stilbocarpa polaris&rft_subject=Agrostis magellanica&rft_subject=CAMERA&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=FIELD INVESTIGATION&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data

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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License
(http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_3095_Anatomical_Data when using these data.

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Full description

The dataset contains a series of anatomical images of plant specimens collected as part of AAS (ASAC) project 3095 at Macquarie Island during the summer of the 2011/2012 season.

Species
The four species examined were Azorella macquariensis, Colobanthus muscoides, Stilbocarpa polaris and Agrostis magellanica. Samples labelled 'South' were from Hurd point either near the hut (Colobanthus Stilbocarp and Agrostis) or from near the track at the top of the Hurd Point jump-up. The remaining plants were collected from the northern end of the island near the station.

Anatomy and Leaf area
The anatomy of the four species were investigated by taking hand cut sections and where necessary, staining with Toluidine Blue (0.05% in distilled water). Both transverse and longitudinal sections were taken. Photographic images were taken using Nikon Coolpic camera with eye tube adjustment. Images were accompanied with stage micrometre images. Two microscopes were used: a dissecting Leica MZ 8 and a Zeiss Axioscope. In some samples corresponding leaf surfaces were photographed of leaves removed from branches above the location of the transverse sections to calculate xylem surface area to leaf area ratios. Leaves were removed from the branches and places on graph paper under glass slides and photographed. Scales of the graph paper is 1 x 1 mm.

Rehydration sequence
Rehydration sequences of xylem vessels in both Colobanthus and Azorella samples were created by dissecting out xylem strands from longitudinal sections and drying on a glass slide. Water was then applied to rehydrate the cells and the sequence of embolism collapse was observed and photographed at defined time intervals. Interval measure had some non-measured margin of error as the shutter control on the camera was manually operated. Repeat drying and rehydration sequences were performed on the same sample.

Two plants labelled Azorella macquariensis MC Ball experimental plant 1 and MC Ball Azorella no 2 Kleaf have associated physiological experimental data collected from them by Marilyn Ball.

Lineage

Progress Code: completed

Data time period: 2011-11-20 to 2011-12-19

This dataset is part of a larger collection

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text: westlimit=158.86; southlimit=-54.65; eastlimit=158.862; northlimit=-54.59

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Other Information

Request point for the dataset (GET DATA)

uri : http://data.aad.gov.au/aadc/portal/download_file.cfm?file_id=3448 External Link

Public information for ASAC project 3095 (PROJECT HOME PAGE)

uri : https://projects.aad.gov.au/search_projects_results.cfm?project_no=3095 External Link

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

uri : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_3095_Anatomical_Data External Link

Identifiers

URI : data.aad.gov.au/metadata/records/ASAC_3095_Anatomical_Data
Local : ASAC_3095_Anatomical_Data
DOI : 10.4225/15/54AF2ECD05BBD
global : 3e497247-4968-4c12-ac4a-09ac72d42d73