Data

Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes

James Cook University
Gierz, Sarah
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/28/598a96f71288a&rft.title=Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes&rft.identifier=10.4225/28/598a96f71288a&rft.publisher=James Cook University&rft.description=Chapter 2 of the author’s PhD thesis is published in the journal Frontiers in Plant Science. This study describes the transcriptome response of Symbiodinium sp. (clade F) cultures exposed to thermal stress (four, nineteen and twenty-eight days) at future temperature conditions and is linked with physiological changes observed. Within the differentially expressed genes, transcripts with significance to the stress response of Symbiodinium were detected. The results indicate a shift in metabolism, from carbon fixation to fatty acid catabolism, supported by upregulation of β-oxidation, glyoxylate cycle and gluconeogenic enzymes, this to our knowledge, has not previously been reported in Symbiodinium. The implications of altered metabolic processes from exposure to thermal stress found in this study, on coral – Symbiodinium associations has not been explored. This study provides an important reference for understanding the mechanisms of coral bleaching at future temperature conditions. The Illumina sequenced read data reported in this article have been deposited into the National Center for Biotechnology Information (NCBI) Sequence Read Archive under the accession number SRA467551, which is associated with BioProject number PRJNA342240. The associated analysed data included as appendices of this thesis may be found in the supplementary information of the publication or attached as MS Excel sheets.&rft.creator=Gierz, Sarah &rft.date=2017&rft.relation=http://dx.doi.org/10.3389/fpls.2017.00271&rft.relation=http://dx.doi.org/10.1038/srep25081&rft.relation=https://researchonline.jcu.edu.au/51805/&rft_rights=&rft_rights=CC BY: Attribution 3.0 AU http://creativecommons.org/licenses/by/3.0/au&rft_subject=Symbiodinium&rft_subject=gene expression&rft_subject=RNA-Seq&rft_subject=transcriptome&rft_subject=thermal stress&rft_subject=ARC Centre of Excellence for Coral Reef Studies&rft.type=dataset&rft.language=English Access the data

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Chapter 2 of the author’s PhD thesis is published in the journal Frontiers in Plant Science. This study describes the transcriptome response of Symbiodinium sp. (clade F) cultures exposed to thermal stress (four, nineteen and twenty-eight days) at future temperature conditions and is linked with physiological changes observed. Within the differentially expressed genes, transcripts with significance to the stress response of Symbiodinium were detected. The results indicate a shift in metabolism, from carbon fixation to fatty acid catabolism, supported by upregulation of β-oxidation, glyoxylate cycle and gluconeogenic enzymes, this to our knowledge, has not previously been reported in Symbiodinium. The implications of altered metabolic processes from exposure to thermal stress found in this study, on coral – Symbiodinium associations has not been explored. This study provides an important reference for understanding the mechanisms of coral bleaching at future temperature conditions. The Illumina sequenced read data reported in this article have been deposited into the National Center for Biotechnology Information (NCBI) Sequence Read Archive under the accession number SRA467551, which is associated with BioProject number PRJNA342240. The associated analysed data included as appendices of this thesis may be found in the supplementary information of the publication or attached as MS Excel sheets.

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This dataset consists of 5 spreadsheets in MS Excel (.xlsx) and Open Document formats (.ods)

Created: 2017-02-14

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  • DOI : 10.4225/28/598A96F71288A
  • Local : researchdata.jcu.edu.au//published/218fbba778179fc25520719828c10a62
  • Local : b9907243d0b928b8b36be8be8ef043ed