Data

Trace element concentration in whale faeces and muscle, and krill tissue, collected from the Southern Ocean

Australian Ocean Data Network
Ratnarajah, Lavenia ; Bowie, Andrew, Dr ; Lannuzel, Delphine ; Meiners, Klaus ; Nicol, Steve, Prof.
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=c744388c-b914-4771-99e2-870bd8114e30&rft.title=Trace element concentration in whale faeces and muscle, and krill tissue, collected from the Southern Ocean&rft.identifier=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=c744388c-b914-4771-99e2-870bd8114e30&rft.description=Whale muscle samples were collected from stranded and dead blue (Baleoptera musculus) and fin (Baleoptera physalus) whales in South-western Australia. Blue, fin, sperm (Physeter macrocephalus), humpback (Megaptera novaeangliae) and pygmy blue (Baleoptera musculus brevicauda) whale faecal samples were collected from coastal waters off Southern Australia by trawling 0.5 mm mesh nets over the surface waters following defecation. Four species of krill (Nyctiphanes australia, Euphausia pacifica, Meganyctiphanes norvegica), including Antarctic krill (Euphausia superba) were collected from various locations worldwide. We analysed the concentration of iron, cadmium, manganese, cobalt, copper, zinc, phosphorus and carbon in baleen whale faeces and muscle, and krill tissue using inductively coupled plasma mass spectrometry.1. Sample collection All sample tissue and faecal matter were stored in individual 50 ml polycarbonate screw cap bottles, preserved in >70% ethanol and frozen at -20°C until analyses. 2. Analysis of the trace element concentration Samples were dried at 60°C until constant weight was attained. Subsequently they were crushed using an acid-cleaned pipette tip and shaken vigorously to homogenise the samples. Digestion of 2–100 mg subsamples were performed in acid-cleaned 15 ml Teflon® perfluoroalkoxy (PFA) vials (Savillex, Minnetonka, MN, USA) by adding 1 ml of concentrated nitric acid and 0.125 ml of hydrogen peroxide (all Ultrapure, Seastar Baseline®, Choice Analytical). The samples were then heated at 125°C for 8 hours on Teflon coated digestion hotplate, housed in a bench-top fume hood coupled with HEPA filters to ensure clean input air (Digiprep, France). Identical procedures were applied to blanks (n = 6) and to two certified referenced materials (n = 5) (DORM-3 fish protein; National Research Council, Ottawa, Canada; and NIST 1566a oyster tissue; National Institute of Standards and Technology, Gaithersburg, Maryland, USA). Certified materials, blanks and samples were resuspended in 10–100 mL of 10% v:v nitric acid (Ultrapure, Seastar Baseline) and analysed by sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) (Finnigan MAT ELEMENT 1 Bremen Germany), following methods described in Cullen and Sherrell (1999) and Townsend (2000). 3. Analysis of carbon All glass- and metal-ware in contact with the carbon samples were pre-combusted at 450°C for 12 hours. Subsamples (2–100 mg) of dried faecal matter were placed in 13 mm diameter silver capsules (Sercon, Australia) and carbon content was then determined at the Central Science Laboratory, University of Tasmania, using a Thermo Finnigan EA 1112 Series Flash Elemental Analyser (estimated precision ~1%). Note, for some samples the date of collection was not recorded.&rft.creator=Ratnarajah, Lavenia &rft.creator=Bowie, Andrew, Dr &rft.creator=Lannuzel, Delphine &rft.creator=Meiners, Klaus &rft.creator=Nicol, Steve, Prof. &rft.date=2020&rft.coverage=northlimit=-33.00; southlimit=-85.00; westlimit=-180.00; eastLimit=180.00&rft.coverage=northlimit=-33.00; southlimit=-85.00; westlimit=-180.00; eastLimit=180.00&rft_rights=Creative Commons Attribution 2.5 Australia License http://creativecommons.org/licenses/by/2.5&rft_subject=oceans&rft_subject=Baleen Whale&rft_subject=Blue Whale&rft_subject=Baleoptera musculus&rft_subject=Fin Whale&rft_subject=Baleoptera physalus&rft_subject=Sperm Whale&rft_subject=Physeter macrocephalus&rft_subject=Humpback Whale&rft_subject=Megaptera novaeangliae&rft_subject=Pygmy Blue Whale&rft_subject=Baleoptera musculus brevicauda&rft_subject=Krill&rft_subject=Nyctiphanes australia&rft_subject=Euphausia pacifica&rft_subject=Meganyctiphanes norvegica&rft_subject=Antarctic krill&rft_subject=Euphausia superba&rft_subject=Global/Oceans | Southern Ocean&rft_subject=Continents | Antarctica&rft_subject=Faeces&rft_subject=Muscle&rft_subject=Tissue&rft_subject=Mass Spectrometers&rft_subject=Nets | Surface Nets&rft_subject=BALEEN WHALES&rft_subject=EUPHAUSIIDS (KRILL)&rft_subject=CRUSTACEANS&rft_subject=NUTRIENTS&rft_subject=TRACE ELEMENTS&rft_subject=CARBON&rft_subject=MAMMALS&rft_subject=ANIMALS/INVERTEBRATES&rft_subject=ZOOPLANKTON&rft_subject=Biological Oceanography&rft_subject=EARTH SCIENCES&rft_subject=OCEANOGRAPHY&rft_subject=research vessel&rft_subject=Concentration of Iron&rft_subject=Concentration of Cadmium&rft_subject=Concentration of Manganese&rft_subject=Concentration of Cobalt&rft_subject=Concentration of Copper&rft_subject=Concentration of Zinc&rft_subject=Concentration of phosphorus&rft_subject=Concentration of Carbon&rft.type=dataset&rft.language=English Access the data

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Brief description

Whale muscle samples were collected from stranded and dead blue (Baleoptera musculus) and fin (Baleoptera physalus) whales in South-western Australia. Blue, fin, sperm (Physeter macrocephalus), humpback (Megaptera novaeangliae) and pygmy blue (Baleoptera musculus brevicauda) whale faecal samples were collected from coastal waters off Southern Australia by trawling 0.5 mm mesh nets over the surface waters following defecation. Four species of krill (Nyctiphanes australia, Euphausia pacifica, Meganyctiphanes norvegica), including Antarctic krill (Euphausia superba) were collected from various locations worldwide.

We analysed the concentration of iron, cadmium, manganese, cobalt, copper, zinc, phosphorus and carbon in baleen whale faeces and muscle, and krill tissue using inductively coupled plasma mass spectrometry.

Lineage

1. Sample collection
All sample tissue and faecal matter were stored in individual 50 ml polycarbonate screw cap bottles, preserved in >70% ethanol and frozen at -20°C until analyses.

2. Analysis of the trace element concentration
Samples were dried at 60°C until constant weight was attained. Subsequently they were crushed using an acid-cleaned pipette tip and shaken vigorously to homogenise the samples. Digestion of 2–100 mg subsamples were performed in acid-cleaned 15 ml Teflon® perfluoroalkoxy (PFA) vials (Savillex, Minnetonka, MN, USA) by adding 1 ml of concentrated nitric acid and 0.125 ml of hydrogen peroxide (all Ultrapure, Seastar Baseline®, Choice Analytical). The samples were then heated at 125°C for 8 hours on Teflon coated digestion hotplate, housed in a bench-top fume hood coupled with HEPA filters to ensure clean input air (Digiprep, France). Identical procedures were applied to blanks (n = 6) and to two certified referenced materials (n = 5) (DORM-3 fish protein; National Research Council, Ottawa, Canada; and NIST 1566a oyster tissue; National Institute of Standards and Technology, Gaithersburg, Maryland, USA). Certified materials, blanks and samples were resuspended in 10–100 mL of 10% v:v nitric acid (Ultrapure, Seastar Baseline) and analysed by sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) (Finnigan MAT ELEMENT 1 Bremen Germany), following methods described in Cullen and Sherrell (1999) and Townsend (2000).

3. Analysis of carbon
All glass- and metal-ware in contact with the carbon samples were pre-combusted at 450°C for 12 hours. Subsamples (2–100 mg) of dried faecal matter were placed in 13 mm diameter silver capsules (Sercon, Australia) and carbon content was then determined at the Central Science Laboratory, University of Tasmania, using a Thermo Finnigan EA 1112 Series Flash Elemental Analyser (estimated precision ~1%).


Note, for some samples the date of collection was not recorded.

Notes

Credit
Antarctic Climate and Ecosystem Cooperative Research Centre (ACE CRC)
Credit
Australian Antarctic Division (AAD)
Credit
Australian Marine Mammal Centre
Credit
Institute for Marine and Antarctic Studies (IMAS)
Purpose
To determine the elemental composition (iron, cadmium, manganese, cobalt, copper, zinc, phosphorus, carbon) of baleen whale tissue and krill from the southern ocean.

Data time period: 01 01 1997

180,-33 180,-85 0,-85 -180,-85 -180,-33 0,-33 180,-33

0,-59

text: northlimit=-33.00; southlimit=-85.00; westlimit=-180.00; eastLimit=180.00

Other Information
(DATA - Whale and krill trace element concentration [direct download])

uri : https://data.imas.utas.edu.au/attachments/c744388c-b914-4771-99e2-870bd8114e30/Whale_and_krill_trace_element_concentration_mgkg_RATNARAJAH.xlsx

MAP - region of tissue collection in the Southern Ocean (imas:ACE_LRatnarajah_Trace_element_concentration_whale_faeces_southern_ocean_GV)

uri : https://geoserver.imas.utas.edu.au/geoserver/wms

(View and download this data through the interactive IMAS Data Portal.)

uri : https://data.imas.utas.edu.au/portal/search

URI : https://data.imas.utas.edu.au/portal/search?uuid=c744388c-b914-4771-99e2-870bd8114e30

Identifiers
  • global : c744388c-b914-4771-99e2-870bd8114e30