Data

Total and hydrocarbon-degrading bacteria in the permeable reactive barrier at Casey

Australian Antarctic Division
Powell, S., Rayner, J. and Bowman, J. ; POWELL, SHANE ; BOWMAN, JOHN ; RAYNER, JOHN LENDON
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5c9b0907756dd&rft.title=Total and hydrocarbon-degrading bacteria in the permeable reactive barrier at Casey&rft.identifier=10.26179/5c9b0907756dd&rft.publisher=Australian Antarctic Data Centre&rft.description=Material was collected from the permeable reactive barrier that was installed at the Main Power House spill site at Casey Station, Antarctica. Material from the barrier was collected from 40 sites throughout the barrier and at varying depths.Samples of the raw material GAC (granulated activated carbon) and Zeopro and three soil samples collected upgradient from the barrier are also included.The numbers of total heterotrophic bacteria were determined using a most-probable-number count procedure with half-strength tryptone-soya broth and the numbers of Special Antarctic diesel - degrading bacteria were determined using Bushnell-Haas medium with SAB as the sole carbon source.Numbers are presented as the most-probable number per gram of wet barrier material.This work was completed as part of ASAC projects 1163 and 2672 (ASAC_1163, ASAC_2672).More information about the dataset is presented on the summary worksheet of the download file - this information is copied below:Most probable number counts were carried out on material sampled from the permeable reactive barrier at Casey in Jan/Feb 2007. 4-tube MPN with three replicates for each sample. Most probable number was calculated using the MPN Calculator available from: http:/members.ync.net/mcuriale/mpn/index.htmlThe barcode numbers are barcodes recorded in the Human Impacts Sample Tracking Database.The sample name has the multiport location and depth of sample in m (eg MP01 0.45 is multiport 1 at 0.45m depth).Total heterotrophs were estimated using half-strength tryptone-soya broth in 96-well titre trays.1:10 serial dilutions were made in a total of 200 microlitres of medium.Plates were incubated at 10°C for 7 days. Plates were scored manually with visually turbid cells being positive.SAB-degrading bacteria were estimated using Bushnell-Haas broth and 5ul of SAB as carbon source.1:10 serial dilutions were made in a total of 200 microlitres of medium.Plates were incubated at 10 degrees C for 14 days. At this time 40 microlitres of INT solution were added and incubated for another two days (INT = 2.25 g/l of iodonitrotetrazolium chloride).Plates were scored manually, with the presence of a red precipitate or red colour being positive.Progress Code: completed&rft.creator=Powell, S., Rayner, J. and Bowman, J. &rft.creator=POWELL, SHANE &rft.creator=BOWMAN, JOHN &rft.creator=RAYNER, JOHN LENDON &rft.date=2007&rft.coverage=westlimit=110.5; southlimit=-66.3; eastlimit=110.54; northlimit=-66.26&rft.coverage=westlimit=110.5; southlimit=-66.3; eastlimit=110.54; northlimit=-66.26&rft.coverage=uplimit=0.6; downlimit=0.1&rft.coverage=uplimit=0.6; downlimit=0.1&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=PRB_bacterialnumbers_2007 when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=environment&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > TERRESTRIAL ECOSYSTEMS&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > CONTAMINANT LEVELS/SPILLS&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS&rft_subject=EARTH SCIENCE > LAND SURFACE > SOILS > CARBON&rft_subject=EARTH SCIENCE > LAND SURFACE > SOILS > RECLAMATION/REVEGETATION/RESTORATION&rft_subject=EARTH SCIENCE > SOLID EARTH > EARTH GASES/LIQUIDS > PETROLEUM&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA&rft_subject=hydrocarboon biodegradation&rft_subject=zeolite&rft_subject=fuel spill&rft_subject=bioremediation&rft_subject=permeable reactive barrier&rft_subject=granulated activated carbon&rft_subject=heterotrophic bacteria&rft_subject=diesel&rft_subject=special antarctic blend&rft_subject=TRAPS&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=CONTINENT > ANTARCTICA > Casey&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
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These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=PRB_bacterialnumbers_2007 when using these data.

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Full description

Material was collected from the permeable reactive barrier that was installed at the Main Power House spill site at Casey Station, Antarctica. Material from the barrier was collected from 40 sites throughout the barrier and at varying depths.

Samples of the raw material GAC (granulated activated carbon) and Zeopro and three soil samples collected upgradient from the barrier are also included.

The numbers of total heterotrophic bacteria were determined using a most-probable-number count procedure with half-strength tryptone-soya broth and the numbers of Special Antarctic diesel - degrading bacteria were determined using Bushnell-Haas medium with SAB as the sole carbon source.

Numbers are presented as the most-probable number per gram of wet barrier material.

This work was completed as part of ASAC projects 1163 and 2672 (ASAC_1163, ASAC_2672).

More information about the dataset is presented on the summary worksheet of the download file - this information is copied below:

Most probable number counts were carried out on material sampled from the permeable reactive barrier at Casey in Jan/Feb 2007. 4-tube MPN with three replicates for each sample. Most probable number was calculated using the MPN Calculator available from: http:/members.ync.net/mcuriale/mpn/index.html

The barcode numbers are barcodes recorded in the Human Impacts Sample Tracking Database.
The sample name has the multiport location and depth of sample in m (eg MP01 0.45 is multiport 1 at 0.45m depth).

Total heterotrophs were estimated using half-strength tryptone-soya broth in 96-well titre trays.
1:10 serial dilutions were made in a total of 200 microlitres of medium.
Plates were incubated at 10°C for 7 days. Plates were scored manually with visually turbid cells being positive.

SAB-degrading bacteria were estimated using Bushnell-Haas broth and 5ul of SAB as carbon source.
1:10 serial dilutions were made in a total of 200 microlitres of medium.
Plates were incubated at 10 degrees C for 14 days. At this time 40 microlitres of INT solution were added and incubated for another two days (INT = 2.25 g/l of iodonitrotetrazolium chloride).
Plates were scored manually, with the presence of a red precipitate or red colour being positive.

Lineage

Progress Code: completed

Data time period: 2007-01-15 to 2007-02-28

This dataset is part of a larger collection

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110.54,-66.26 110.54,-66.3 110.5,-66.3 110.5,-66.26 110.54,-66.26

110.52,-66.28

text: westlimit=110.5; southlimit=-66.3; eastlimit=110.54; northlimit=-66.26

text: uplimit=0.6; downlimit=0.1

Subjects
AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA > Casey | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > TERRESTRIAL ECOSYSTEMS | EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > CONTAMINANT LEVELS/SPILLS | EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS | EARTH SCIENCE > LAND SURFACE > SOILS > CARBON | EARTH SCIENCE > LAND SURFACE > SOILS > RECLAMATION/REVEGETATION/RESTORATION | EARTH SCIENCE > SOLID EARTH > EARTH GASES/LIQUIDS > PETROLEUM | FIELD SURVEYS | GEOGRAPHIC REGION > POLAR | LABORATORY | TRAPS | bioremediation | diesel | environment | fuel spill | granulated activated carbon | heterotrophic bacteria | hydrocarboon biodegradation | permeable reactive barrier | special antarctic blend | zeolite |

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Other Information
Download point for the data - excel file (GET DATA)

url : https://data.aad.gov.au/eds/1536/download

Public information for ASAC project 1163 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=1163

Public information for ASAC project 2672 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=2672

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=PRB_bacterialnumbers_2007

Identifiers
ACN 633 798 857