Data

Torres Strait, CORAL Project, September 02-06, 2016, HPLC Pigment and Ocean Colour Data

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) CSIRO/Oceans and Atmosphere (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/064bff02-7ba3-46e0-b873-afdf387a1205&rft.title=Torres Strait, CORAL Project, September 02-06, 2016, HPLC Pigment and Ocean Colour Data&rft.identifier=064bff02-7ba3-46e0-b873-afdf387a1205&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data generated from 19 stations in the Torres Strait between September 2, 2016 and September 6, 2016 using the vessel, Eclipse D. CSIRO acquired bio-optical measurements in the Torres Strait and Great Barrier Reef, as part of the NASA Jet Propulsion Laboratory (JPL) Earth Venture Suborbital mission: Coral Reef Airborne Laboratory (CORAL, http://coral.jpl.nasa.gov). The goal of this airborne hyperspectral remote sensing project is to provide critical data and new models needed to analyse the status of coral reefs and to predict their future status. The CORAL mission includes PRISM (Portable Remote Imaging Spectrometer) airborne hyperspectral transects over portions of the southern and northern Great Barrier Reef (GBR), including the Torres Strait. Parameters measured include Total Suspended Particulate Matter (TSM), including the organic and inorganic fractions; the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition. The data can be used for Ocean Colour sensor validation.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=142.003; southlimit=-10.538; eastlimit=142.396; northlimit=-9.738; projection=WGS 84 (EPSG:4326)&rft.coverage=westlimit=142.003; southlimit=-10.538; eastlimit=142.396; northlimit=-9.738; projection=WGS 84 (EPSG:4326)&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=WfO Biogeochemistry&rft_subject=Marine Features (Australia) | Torres Strait, QLD&rft_subject=Global / Oceans | Australasia&rft_subject=Marine Features (Australia) | Great Barrier Reef, QLD&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Filtration Systems&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data generated from 19 stations in the Torres Strait between September 2, 2016 and September 6, 2016 using the vessel, Eclipse D. CSIRO acquired bio-optical measurements in the Torres Strait and Great Barrier Reef, as part of the NASA Jet Propulsion Laboratory (JPL) Earth Venture Suborbital mission: Coral Reef Airborne Laboratory (CORAL, http://coral.jpl.nasa.gov). The goal of this airborne hyperspectral remote sensing project is to provide critical data and new models needed to analyse the status of coral reefs and to predict their future status. The CORAL mission includes PRISM (Portable Remote Imaging Spectrometer) airborne hyperspectral transects over portions of the southern and northern Great Barrier Reef (GBR), including the Torres Strait. Parameters measured include Total Suspended Particulate Matter (TSM), including the organic and inorganic fractions; the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition. The data can be used for Ocean Colour sensor validation.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.

Notes

Credit
Janet Anstee (O&A)

Data time period: 2016-09-02 to 2016-09-06

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142.396,-9.738 142.396,-10.538 142.003,-10.538 142.003,-9.738 142.396,-9.738

142.1995,-10.138

text: westlimit=142.003; southlimit=-10.538; eastlimit=142.396; northlimit=-9.738; projection=WGS 84 (EPSG:4326)

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Filtration Systems | Fluorometers | Global / Oceans | Australasia | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Great Barrier Reef, QLD | Marine Features (Australia) | Torres Strait, QLD | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

url : https://aesop.csiro.au/

(NASA CORAL)

url : https://coral.jpl.nasa.gov/about-coral

Download Torres Strait Pigment Data (Pigment Data)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=3784

Data also available via IMOS and AODN portal (IMOS and AODN portal)

url : https://portal.aodn.org.au/

Identifiers
  • global : 064bff02-7ba3-46e0-b873-afdf387a1205