Data

The Biochemistry, Ecology and Organic Geochemistry of Phaeocystis pouchetii communities of Davis Base, Antarctica

Australian Antarctic Division
Nichols. P. and Skerratt, J.H. ; NICHOLS, PETER ; SKERRATT, JENNIFER HELEN
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/ASAC_113&rft.title=The Biochemistry, Ecology and Organic Geochemistry of Phaeocystis pouchetii communities of Davis Base, Antarctica&rft.identifier=http://data.aad.gov.au/metadata/ASAC_113&rft.publisher=Australian Antarctic Data Centre&rft.description=This metadata record covers ASAC projects 113, 191 and 625. (ASAC_113, ASAC_191, ASAC_625).The total lipid, fatty acid, sterol and pigment composition of water column particulates collected near the Australian Antarctic Base, Davis Station, were analysed over five summer seasons (1988-93) using capillary GC, GC-MS, TLC-FID and HPLC. Polar lipids were the dominant lipid class. Maximum lipid concentrations usually occurred in samples collected in December and January and corresponded with increased algal biomass. Both lipid profiles and microscopic observations showed significant variation in algal biomass and community structure in the water column during each season and on an interannual basis. During the period of diatom blooms (predominantly Nitzschia species) the dominant sterol and fatty acid were trans-22-dehydrocholesterol and 20:5w3, accompanied by a high 16:1w7 to 16:0 ratio. Very high polyunsaturated fatty acid and total lipid concentrations were associated with diatom blooms in the area. Bacterial markers increased late in all seasons after the summer algal blooms. Long chain C30 sterols also increased during the latter half of all seasons. Fjord samples collected in the area reflected greater biomass and diversity in algal and bacterial makers than coastal sites. Signature lipids for the alga Phaeocystis pouchetii, thought to be a major alga in Antarctic waters, were identified in field samples over the five summer seasons studied.MethodsStudy siteDavis Base is situated on the Vestfold Hills, Antarctica and incorporates numerous lakes and fjords (Fig. 1). Samples of water column particulate matter were collected during five summer seasons (1988-93), 500 meters off-shore from Magnetic Island, situated 5 km NW of Davis.Three other sampling areas were situated in the fjords of the Vestfold hills and include two sites in Ellis Fjord, one midway along Ellis Fjord and one near Ellis Fjord mouth and one sample midway along Long Fjord (Fig. 1). These fjords are protected from the marine environment, but are both marine fjords.Davis Station and Magnetic Island were used for the weekly sample sites. The mouth of Long Fjord, the mouth of Ellis Fjord, midway down Long Fjord, the deep basin in Ellis Fjord, O'Gorman Rocks and Hawker island (ocean side) were used for monthly samples.Field collectionThere was an initial pilot season in 1988-89, which was followed by two more detailed studies in the summers of 1989-90 and 1990-91. Four samples was also analysed from the 1991-92 and five from the 1992-93 summer seasons. During the initial pilot study at Magnetic Island in the 1988-89 summer, three water column particle samples were taken for lipid analyses. The 1989-90 and 1990-91 summer field seasons incorporated weekly sampling of the water column particulates at Magnetic Island. The phytoplankton in the fjords were studied during the summers of 1989-90 and 1990-91. The three sites that were chosen were all sampled three times in each season. Samples were also collected during the 1989-90 and 1990-91 seasons from the Magnetic Island and Fjord site s for pigment analyses. Three and five samples were collected respectively in the 1991-92 and 1992-93 seasons. Samples were also taken for microscopic analyses.For lipid analyses 30-40 liter water column particulate samples were collected at a depth of 10 m. A Seastar or INFILTREX water sampler was used in situ to filter the water through a 14.2 cm Schleicher and Schuell glass fibre filter over a three to four hour period. All filters used during sampling were preheated in a muffle furnace at 500 degrees C overnight to minimise contamination.For pigment analyses 2 to 4 litres were filtered through glass fibre filters (4.7 cm GF/F, nominal pore size 0.7 micro meters). The samples were frozen at -20 degrees C until extraction.Progress Code: completed&rft.creator=Nichols. P. and Skerratt, J.H. &rft.creator=NICHOLS, PETER &rft.creator=SKERRATT, JENNIFER HELEN &rft.date=2000&rft.coverage=westlimit=77.87497; southlimit=-68.61744; eastlimit=78.06497; northlimit=-68.53744&rft.coverage=westlimit=77.87497; southlimit=-68.61744; eastlimit=78.06497; northlimit=-68.53744&rft.coverage=uplimit=10; downlimit=10&rft.coverage=uplimit=10; downlimit=10&rft_rights=A copy of one of the resulting publications is available for AAD staff only to download at the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_113 when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=geoscientificInformation&rft_subject=oceans&rft_subject=EARTH SCIENCE > CRYOSPHERE > SEA ICE > SEA ICE AGE&rft_subject=EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > BIOGEOCHEMICAL CYCLES&rft_subject=EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE > DIATOMS&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOGEOCHEMICAL CYCLES&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > FOOD-WEB DYNAMICS&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > TROPHIC DYNAMICS&rft_subject=ALGAE&rft_subject=BACTERIA&rft_subject=BIOGEOCHEMICAL CYCLES&rft_subject=FATTY ACIDS&rft_subject=LIPID&rft_subject=PHAEOCYSTIS&rft_subject=STEROLS&rft_subject=WATER COLUMN&rft_subject=GRAB SAMPLERS&rft_subject=ICE AUGERS&rft_subject=KEMMERER SAMPLER&rft_subject=SALINOMETERS&rft_subject=WATER BOTTLES&rft_subject=GAS CHROMATOGRAPHS&rft_subject=MICROSCOPES&rft_subject=ICP-AES > Inductively Coupled Plasma Atomic Emission Spectrometer&rft_subject=FIELD SURVEYS&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=CONTINENT > ANTARCTICA > Davis Station&rft_subject=CONTINENT > ANTARCTICA > Magnetic Island&rft_subject=CONTINENT > ANTARCTICA > Long Fjord&rft_subject=CONTINENT > ANTARCTICA > Ellis fjord&rft_subject=CONTINENT > ANTARCTICA > O'Gorman Rocks&rft_subject=CONTINENT > ANTARCTICA > Hawker island&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
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A copy of one of the resulting publications is available for AAD staff only to download at the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_113 when using these data.

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Full description

This metadata record covers ASAC projects 113, 191 and 625. (ASAC_113, ASAC_191, ASAC_625).

The total lipid, fatty acid, sterol and pigment composition of water column particulates collected near the Australian Antarctic Base, Davis Station, were analysed over five summer seasons (1988-93) using capillary GC, GC-MS, TLC-FID and HPLC. Polar lipids were the dominant lipid class. Maximum lipid concentrations usually occurred in samples collected in December and January and corresponded with increased algal biomass. Both lipid profiles and microscopic observations showed significant variation in algal biomass and community structure in the water column during each season and on an interannual basis. During the period of diatom blooms (predominantly Nitzschia species) the dominant sterol and fatty acid were trans-22-dehydrocholesterol and 20:5w3, accompanied by a high 16:1w7 to 16:0 ratio. Very high polyunsaturated fatty acid and total lipid concentrations were associated with diatom blooms in the area. Bacterial markers increased late in all seasons after the summer algal blooms. Long chain C30 sterols also increased during the latter half of all seasons. Fjord samples collected in the area reflected greater biomass and diversity in algal and bacterial makers than coastal sites. Signature lipids for the alga Phaeocystis pouchetii, thought to be a major alga in Antarctic waters, were identified in field samples over the five summer seasons studied.

Methods
Study site

Davis Base is situated on the Vestfold Hills, Antarctica and incorporates numerous lakes and fjords (Fig. 1). Samples of water column particulate matter were collected during five summer seasons (1988-93), 500 meters off-shore from Magnetic Island, situated 5 km NW of Davis.

Three other sampling areas were situated in the fjords of the Vestfold hills and include two sites in Ellis Fjord, one midway along Ellis Fjord and one near Ellis Fjord mouth and one sample midway along Long Fjord (Fig. 1). These fjords are protected from the marine environment, but are both marine fjords.

Davis Station and Magnetic Island were used for the weekly sample sites. The mouth of Long Fjord, the mouth of Ellis Fjord, midway down Long Fjord, the deep basin in Ellis Fjord, O'Gorman Rocks and Hawker island (ocean side) were used for monthly samples.

Field collection
There was an initial pilot season in 1988-89, which was followed by two more detailed studies in the summers of 1989-90 and 1990-91. Four samples was also analysed from the 1991-92 and five from the 1992-93 summer seasons. During the initial pilot study at Magnetic Island in the 1988-89 summer, three water column particle samples were taken for lipid analyses. The 1989-90 and 1990-91 summer field seasons incorporated weekly sampling of the water column particulates at Magnetic Island. The phytoplankton in the fjords were studied during the summers of 1989-90 and 1990-91. The three sites that were chosen were all sampled three times in each season. Samples were also collected during the 1989-90 and 1990-91 seasons from the Magnetic Island and Fjord site s for pigment analyses. Three and five samples were collected respectively in the 1991-92 and 1992-93 seasons. Samples were also taken for microscopic analyses.

For lipid analyses 30-40 liter water column particulate samples were collected at a depth of 10 m. A Seastar or INFILTREX water sampler was used in situ to filter the water through a 14.2 cm Schleicher and Schuell glass fibre filter over a three to four hour period. All filters used during sampling were preheated in a muffle furnace at 500 degrees C overnight to minimise contamination.

For pigment analyses 2 to 4 litres were filtered through glass fibre filters (4.7 cm GF/F, nominal pore size 0.7 micro meters). The samples were frozen at -20 degrees C until extraction.

Lineage

Progress Code: completed

Data time period: 1988-02-02 to 1989-02-14

Data time period: 1989-12-13 to 1990-01-12

Data time period: 1990-10-17 to 1991-02-01

Data time period: 1992-10-21 to 1993-01-23

This dataset is part of a larger collection

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78.06497,-68.53744 78.06497,-68.61744 77.87497,-68.61744 77.87497,-68.53744 78.06497,-68.53744

77.96997,-68.57744

text: westlimit=77.87497; southlimit=-68.61744; eastlimit=78.06497; northlimit=-68.53744

text: uplimit=10; downlimit=10

Subjects
ALGAE | AMD | AMD/AU | BACTERIA | BIOGEOCHEMICAL CYCLES | CEOS | CONTINENT > ANTARCTICA > Davis Station | CONTINENT > ANTARCTICA > Ellis fjord | CONTINENT > ANTARCTICA > Hawker island | CONTINENT > ANTARCTICA > Long Fjord | CONTINENT > ANTARCTICA > Magnetic Island | CONTINENT > ANTARCTICA > O'Gorman Rocks | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE > DIATOMS | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > DIATOMS | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOGEOCHEMICAL CYCLES | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > FOOD-WEB DYNAMICS | EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > TROPHIC DYNAMICS | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC | EARTH SCIENCE > CRYOSPHERE > SEA ICE > SEA ICE AGE | EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > BIOGEOCHEMICAL CYCLES | EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PIGMENTS | FATTY ACIDS | FIELD SURVEYS | GAS CHROMATOGRAPHS | GEOGRAPHIC REGION > POLAR | GRAB SAMPLERS | ICE AUGERS | ICP-AES > Inductively Coupled Plasma Atomic Emission Spectrometer | KEMMERER SAMPLER | LIPID | MICROSCOPES | PHAEOCYSTIS | SALINOMETERS | STEROLS | WATER BOTTLES | WATER COLUMN | biota | geoscientificInformation | oceans |

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Other Information
Public information for ASAC project 113 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=113

Public information for ASAC project 191 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=191

Public information for ASAC project 625 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=625

Download point for the paper - AAD Staff Only (VIEW RELATED INFORMATION > PUBLICATIONS)

url : https://data.aad.gov.au/eds/2909/download

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_113

Identifiers
  • global : ASAC_113
ACN 633 798 857