Data

Data to support Fig 3 of the manuscript "Wide dynamic range of cellular flow through early αβ T-cell selection in the thymus"

Monash University
Rushika C. Wirasinha (Aggregated by) Stephen Daley (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26180/5b9b27134050a&rft.title=Data to support Fig 3 of the manuscript Wide dynamic range of cellular flow through early αβ T-cell selection in the thymus&rft.identifier=http://doi.org/10.26180/5b9b27134050a&rft.publisher=Monash University&rft.description=The file contains mouse T cell receptor (TCR) sequences obtained by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Sequences with a read count of &rft.creator=Rushika C. Wirasinha&rft.creator=Rushika C. Wirasinha&rft.creator=Stephen Daley&rft.creator=Stephen Daley&rft.date=2018&rft_rights=&rft_subject=T cell receptor repertoire&rft_subject=TCR datasets&rft_subject=TCR sequencing&rft_subject=TCR sequence information&rft_subject=T cell selection&rft.type=dataset&rft.language=English Access the data
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The file contains mouse T cell receptor (TCR) sequences obtained by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Sequences with a read count of <3 were excluded. Except for the header row, each row contains information about a unique TCR nucleotide sequence. Columns 1-11 contain output from MIGEC software. Columns 12-15 contain information about sample origin, detailed as follows: column 12 "sample_id" is an identifier for the sample of origin; column 13 "tcr_b" specifies the TCR beta chain status of donor mouse ("poly" = C57BL/6 inbred mouse strain); column 14 “mouse” is an identifier for the individual mouse of origin and column 15 "subset" specifies the T cell subset of origin ("t_1" = Type A IELp thymocytes; "t_4" = CD4+ naive thymocytes; "t_8" = CD8+ naive thymocytes). For materials and methods refer to Wirasinha et al Immunol Cell Biol. 2018 Jul;96(6):553-561. doi: 10.1111/imcb.12047. PMID: 29726044.

Issued: 2018-09-14

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Subjects
T cell receptor repertoire | T cell selection | TCR datasets | TCR sequence information | TCR sequencing |

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Identifiers
ACN 633 798 857