Data

Storm Bay, March 2011, HPLC Pigment data

Integrated Marine Observing System
CSIRO (Associated with) CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/0a0cb241-a135-4a13-8157-09dc5afc3192&rft.title=Storm Bay, March 2011, HPLC Pigment data&rft.identifier=0a0cb241-a135-4a13-8157-09dc5afc3192&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data generated from samples collected on the CMAR vessel Explorer in Storm Bay, Tasmania, between 11 and 16 March 2011. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the Explorer and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere. Absorption and Pigment data was received 31/05/2016 (xls only) and archived c/o O&A Information and Data Centre. The data was archived in the OceanColourData area under StormBay_Derwent_Huon and linked to this record as a zip file.&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=147.116; southlimit=-43.917; eastlimit=148.016; northlimit=-42.061; projection=WGS 84 (EPSG:4326)&rft.coverage=westlimit=147.116; southlimit=-43.917; eastlimit=148.016; northlimit=-42.061; projection=WGS 84 (EPSG:4326)&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=WfO Biogeochemistry&rft_subject=Remote Sensing 2008-2011&rft_subject=Coastal Waters (Australia) | Tasmania Coast East and Southeast, TAS&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Fluorometers&rft_subject=Filtration Systems&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Time Series: Storm Bay - all data&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data generated from samples collected on the CMAR vessel Explorer in Storm Bay, Tasmania, between 11 and 16 March 2011. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the Explorer and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere. Absorption and Pigment data was received 31/05/2016 (xls only) and archived c/o O&A Information and Data Centre. The data was archived in the OceanColourData area under StormBay_Derwent_Huon and linked to this record as a zip file.

Notes

Credit
Lesley Clementson (O&A)
Credit
Christel Hassler (O&A)
Credit
Arnold Dekker (L&W)

Data time period: 2011-03-11 to 2011-03-16

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148.016,-42.061 148.016,-43.917 147.116,-43.917 147.116,-42.061 148.016,-42.061

147.566,-42.989

text: westlimit=147.116; southlimit=-43.917; eastlimit=148.016; northlimit=-42.061; projection=WGS 84 (EPSG:4326)

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | Tasmania Coast East and Southeast, TAS | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Filtration Systems | Fluorometers | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote Sensing 2008-2011 | Spectrophotometers | Temperature of the water body | Time Series: Storm Bay - all data | WfO Biogeochemistry | oceans | research vessel |

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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

url : https://aesop.csiro.au/

Download LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_pigment.zip (.xls only) (BioOptical Storm Bay March 2011 Absorption data)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=141

Data also available via IMOS and AODN portal (IMOS and AODN portal)

url : https://portal.aodn.org.au/

Download LC_IMOS_SRS_BioOptical_Storm_Bay_March_2011_pigment.zip (.xls only) (BioOptical Storm Bay March 2011 Pigment data)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=142

Identifiers
  • global : 0a0cb241-a135-4a13-8157-09dc5afc3192