Data

Southern Surveyor Voyage SS2010_v06 HPLC Pigment data

Integrated Marine Observing System
CSIRO (Associated with) CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/be14733b-06ec-40c1-a14a-ebb3ef8062a8&rft.title=Southern Surveyor Voyage SS2010_v06 HPLC Pigment data&rft.identifier=be14733b-06ec-40c1-a14a-ebb3ef8062a8&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data generated from samples collected from Jurien Bay to Cape Naturaliste, WA, between 31 July and 07 August 2010 on the RV Southern Surveyor voyage ss2010_v06. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere. Data was received on the 31/5/2016 (xls only). The data was archived in the O&A Datacentre in the OceanColourData area under RV_Southern_Surveyor and a copy was placed in Southern_Surveyor_NF under ss2010_v06 to link to this record.&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=114.486; southlimit=-33.502; eastlimit=115.533; northlimit=-30.336; projection=WGS 84 (EPSG:4326)&rft.coverage=westlimit=114.486; southlimit=-33.502; eastlimit=115.533; northlimit=-30.336; projection=WGS 84 (EPSG:4326)&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was collected on voyage [Insert-voyage-name-here] by the Marine National Facility.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=WfO Biogeochemistry&rft_subject=National Facility External Users: C. Pattiaratchi (University of WA) 2000-&rft_subject=Coastal Waters (Australia) | West Australia Coast South, WA&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Fluorometers&rft_subject=Spectrophotometers&rft_subject=Filtration Systems&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Ship: Southern Surveyor&rft_subject=Research Voyage: SS2010_V06&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0
https://creativecommons.org/licenses/by/4.0/

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was collected on voyage [Insert-voyage-name-here] by the Marine National Facility.

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This record contains data generated from samples collected from Jurien Bay to Cape Naturaliste, WA, between 31 July and 07 August 2010 on the RV Southern Surveyor voyage ss2010_v06. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere. Data was received on the 31/5/2016 (xls only). The data was archived in the O&A Datacentre in the OceanColourData area under RV_Southern_Surveyor and a copy was placed in Southern_Surveyor_NF under ss2010_v06 to link to this record.

Notes

Credit
Charitha Pattiaratchi (UWA)
Credit
Christine Hanson (UWA)
Credit
Lesley Clementson (O&A)

Data time period: 2010-07-30 to 2010-08-07

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115.533,-30.336 115.533,-33.502 114.486,-33.502 114.486,-30.336 115.533,-30.336

115.0095,-31.919

text: westlimit=114.486; southlimit=-33.502; eastlimit=115.533; northlimit=-30.336; projection=WGS 84 (EPSG:4326)

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | West Australia Coast South, WA | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Filtration Systems | Fluorometers | HPLC (High Performance Liquid Chromatography) | National Facility External Users: C. Pattiaratchi (University of WA) 2000- | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: SS2010_V06 | Ship: Southern Surveyor | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

url : https://aesop.csiro.au/

Data also available via IMOS and AODN portal (IMOS and AODN portal)

url : https://portal.aodn.org.au/

Download LC_IMOS_SRS_BioOptical_SS062010_pigment.zip (.xls only) (ss2010_v06 BioOptical pigment data)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2372

Identifiers
  • global : be14733b-06ec-40c1-a14a-ebb3ef8062a8