Data

Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of Contact) Clementson, Lesley (Point of Contact)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/f2e138d2-2c51-452c-e043-08114f8cc8ca&rft.title=Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306014370&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected on the RV Southern Surveyor, between July 21 and August 15 2005. The data were collected across two legs. Leg 1 samples were collected between Dampier and Freemantle, and Leg 2 samples were collected between two locations off Freemantle. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate of the water column. 41 stations were sampled. Samples were collected and analysed for pigments.Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis.&rft.creator=Anonymous&rft.date=2014&rft.coverage=northlimit=-21.008; southlimit=-35.436; westlimit=112.251; eastLimit=118.659&rft.coverage=northlimit=-21.008; southlimit=-35.436; westlimit=112.251; eastLimit=118.659&rft_subject=oceans&rft_subject=CHLOROPHYLL&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=OCEAN CHEMISTRY&rft_subject=PIGMENTS&rft_subject=OCEAN COLOR&rft_subject=OCEAN OPTICS&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Coastal Waters (Australia) | West Australia Coast, WA&rft_subject=Global / Oceans | Southern Ocean&rft_subject=WfO Biogeochemistry&rft_subject=Research Voyage: SS 07/2005&rft_subject=WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques&rft_subject=Ship: Southern Surveyor&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Brief description

This record contains data collected on the RV Southern Surveyor, between July 21 and August 15 2005. The data were collected across two legs. Leg 1 samples were collected between Dampier and Freemantle, and Leg 2 samples were collected between two locations off Freemantle. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate of the water column. 41 stations were sampled. Samples were collected and analysed for pigments.

Lineage

Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis.

Notes

Credit
Lesley Clementson

Data time period: 2005-07-21 to 2005-08-15

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118.659,-21.008 118.659,-35.436 112.251,-35.436 112.251,-21.008 118.659,-21.008

115.455,-28.222

text: northlimit=-21.008; southlimit=-35.436; westlimit=112.251; eastLimit=118.659

Subjects
Chlorophyll | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | West Australia Coast, WA | EARTH SCIENCE | Fluorometers | Global / Oceans | Southern Ocean | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | OCEAN CHEMISTRY | Ocean Color | OCEAN OPTICS | OCEANS | Pigments | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: SS 07/2005 | Ship: Southern Surveyor | Spectrophotometers | Temperature of the water body | WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques | WfO Biogeochemistry | oceans | research vessel |

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Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)

uri : https://aesop.csiro.au/

Data also available via IMOS and AODN portal (Data Link)

uri : https://imos.aodn.org.au/webportal/

Download LC_IMOS_SRS_BioOptical_SS07_2005pigments.zip 68KB(.xls, .csv) (Data Link)

uri : https://www.cmar.csiro.au/datacentre/process/data_files/Southern_Surveyor_NF/ss200507/LC_IMOS_SRS_BioOptical_SS07_2005pigments.zip

RV Southern Surveyor Survey Information including voyage plans and summaries (Data Link)

uri : https://www.cmar.csiro.au/data/trawler/survey_list.cfm

URI : https://www.cmar.csiro.au/data/trawler/survey_list.cfm?source_id=8

Marine National Facility (Documentation Link)

uri : https://mnf.csiro.au/

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

uri : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • global : f2e138d2-2c51-452c-e043-08114f8cc8ca
  • Local : Marlin Record Number: 14370
  • Local : Anzlic Identifier: ANZCW0306014370
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