Data

Southern Surveyor Voyage SS 01/99 HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/bcce48b4-d0e3-4fea-bbe8-7b27c8008bff&rft.title=Southern Surveyor Voyage SS 01/99 HPLC Pigment and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306006380&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected at 7 stations on the Southern Surveyor cruise SS 01/99. Water samples were collected from an area of the Southern Ocean near MacQuarie Island to determine the bio-optical properties of water masses in these regions. The data can be used for Ocean Colour and CDOM sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. In addition to the station samples, 47 water samples were collected from the outflow of the underway fluorometer and analysed for the concentration of chlorophyll and carotenoid pigments only.Progress Code: onGoingMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=158; southlimit=-56; eastlimit=160; northlimit=-52&rft.coverage=westlimit=158; southlimit=-56; eastlimit=160; northlimit=-52&rft_rights=Release with the permission of the custodian.&rft_subject=oceans&rft_subject=Earth Science | Biological Classification | Protists | Plankton | Phytoplankton&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Earth Science | Oceans | Ocean Optics | Absorption&rft_subject=Earth Science | Oceans | Ocean Optics | Fluorescence&rft_subject=Earth Science | Oceans | Ocean Optics | Gelbstoff&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Global / Oceans | Southern Ocean&rft_subject=Offshore Islands (Australia) | Macquarie Island&rft_subject=WfO Biogeochemistry&rft_subject=Research Voyage: SS 01/99&rft_subject=Ocean Colour 1997-2000&rft_subject=Ship: Southern Surveyor&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Brief description

This record contains data collected at 7 stations on the Southern Surveyor cruise SS 01/99. Water samples were collected from an area of the Southern Ocean near MacQuarie Island to determine the bio-optical properties of water masses in these regions. The data can be used for Ocean Colour and CDOM sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. In addition to the station samples, 47 water samples were collected from the outflow of the underway fluorometer and analysed for the concentration of chlorophyll and carotenoid pigments only.

Lineage

Progress Code: onGoing
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete

Notes

Credit
Lesley Clementson
Credit
Val Latham
Credit
Alison Turnbull

Data time period: 1999-01-16 to 1999-01-31

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160,-52 160,-56 158,-56 158,-52 160,-52

159,-54

text: westlimit=158; southlimit=-56; eastlimit=160; northlimit=-52

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Earth Science | Biological Classification | Protists | Plankton | Phytoplankton | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Absorption | Earth Science | Oceans | Ocean Optics | Fluorescence | Earth Science | Oceans | Ocean Optics | Gelbstoff | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | Global / Oceans | Southern Ocean | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Ocean Colour 1997-2000 | Offshore Islands (Australia) | Macquarie Island | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: SS 01/99 | Ship: Southern Surveyor | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Other Information
Marine National Facility (Documentation Link)

uri : https://mnf.csiro.au/

Identifiers
  • Local : Anzlic Identifier: ANZCW0306006380
  • Local : Marlin Record Number: 6380
  • global : bcce48b4-d0e3-4fea-bbe8-7b27c8008bff
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