Data

Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) Clementson, Lesley (Point of contact)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/a2683507-5abd-402b-a95e-8e42c47bcd7c&rft.title=Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306006444&rft.publisher=Integrated Marine Observing System&rft.description=This record contains pigment data collected at 53 CTD stations on the Southern Surveyor cruise SS 11/95 in the oceanic waters between 40 and 53 deg south to the east of Tasmania. Eight of these stations were used in an intercomparison pigment study with Japanese scientists from JAMSTEC.At 10 of these stations samples were collected to determine particulate and dissolved absorption coefficients. There were also 15 samples collected from the outflow of the thermosalinography and analysed for pigments.Pigments were also analysed during 7 grazing dilution experiments, 7 sizre fractionation experiments and 7 deployments of free-floating sediment traps. The data were collected to determine the bio-optical charateristics of this region. The data can be used for Ocean Colour and CDOM sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=139; southlimit=-53; eastlimit=152; northlimit=-40&rft.coverage=westlimit=139; southlimit=-53; eastlimit=152; northlimit=-40&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Biological Classification | Protists | Plankton | Phytoplankton&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Absorption&rft_subject=Earth Science | Oceans | Ocean Optics | Fluorescence&rft_subject=Earth Science | Oceans | Ocean Optics | Gelbstoff&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Global / Oceans | Southern Ocean&rft_subject=States, Territories (Australia) | Tasmania&rft_subject=WfO Biogeochemistry&rft_subject=Research Voyage: SS 11/95&rft_subject=Remote sensing of ocean colour - JGFOS 1996-1999&rft_subject=Ship: Southern Surveyor&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

Access:

Open

Full description

This record contains pigment data collected at 53 CTD stations on the Southern Surveyor cruise SS 11/95 in the oceanic waters between 40 and 53 deg south to the east of Tasmania. Eight of these stations were used in an intercomparison pigment study with Japanese scientists from JAMSTEC.At 10 of these stations samples were collected to determine particulate and dissolved absorption coefficients. There were also 15 samples collected from the outflow of the thermosalinography and analysed for pigments.Pigments were also analysed during 7 grazing dilution experiments, 7 sizre fractionation experiments and 7 deployments of free-floating sediment traps. The data were collected to determine the bio-optical charateristics of this region. The data can be used for Ocean Colour and CDOM sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete

Notes

Credit
Lesley Clementson

Data time period: 1995-11-15 to 1995-12-07

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related People

152,-40 152,-53 139,-53 139,-40 152,-40

145.5,-46.5

text: westlimit=139; southlimit=-53; eastlimit=152; northlimit=-40

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Earth Science | Biological Classification | Protists | Plankton | Phytoplankton | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Absorption | Earth Science | Oceans | Ocean Optics | Fluorescence | Earth Science | Oceans | Ocean Optics | Gelbstoff | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | Global / Oceans | Southern Ocean | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote sensing of ocean colour - JGFOS 1996-1999 | Research Voyage: SS 11/95 | Ship: Southern Surveyor | Spectrophotometers | States, Territories (Australia) | Tasmania | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
RV Southern Surveyor Survey Information including voyage plans and summaries (Data Link)

url : https://www.marine.csiro.au/data/trawler/survey_list.cfm?source_id=8

Marine National Facility (Documentation Link)

url : https://mnf.csiro.au/

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

url : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • Local : Anzlic Identifier: ANZCW0306006444
  • Local : Marlin Record Number: 6444
  • global : a2683507-5abd-402b-a95e-8e42c47bcd7c