Brief description
This data was collected on Research Vessel Southern Surveyor, cruise SS 01/95. The voyage departed Hobart, Tasmania on the 14th of January, 1995 and returned to Hobart, Tasmania on the 12th of February, 1995. Samples were collected from 43 stations in order to characterise the optical properties of the Southern Ocean, south and west of Tasmania. Parameters measured include the phytoplankton pigment concentration and composition. The data can be used for Ocean Colour sensor validation.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Notes
CreditBrian Griffiths
Credit
Research carried out on this cruise was partly supported by the Department of Environment, Sports and Territories, through the CSIRO Climate Change Research Program, and the Japanese Global Research Network System.
Research carried out on this cruise was partly supported by the Department of Environment, Sports and Territories, through the CSIRO Climate Change Research Program, and the Japanese Global Research Network System.
text: westlimit=139.879; southlimit=-53.071; eastlimit=144.217; northlimit=-39.953
Subjects
Chlorophyll |
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Climate Change Processes 1991-1995 |
EARTH SCIENCE |
Filtration Systems |
Global / Oceans | Southern Ocean |
HPLC (High Performance Liquid Chromatography) |
Joint Global Ocean Flux Study, IGBP |
Marine Features (Australia) | Great Australian Bight, SA/WA |
Niskin Bottles |
OCEAN CHEMISTRY |
Ocean Color |
OCEAN OPTICS |
OCEANS |
Pigments |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Research Voyage: SS 01/95 |
Ship: Southern Surveyor |
States, Territories (Australia) | Tasmania |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
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Other Information
Cheah, W., McMinn, A., Griffiths, B., Westwood, K.J., Wright, S.W. & Clementson, L. (2013). Response of Phytoplankton Photophysiology to Varying Environmental Conditions in the Sub-Antarctic and Polar Frontal Zone. PloS one. 8. e72165. 10.1371/journal.pone.0072165. (Response of Phytoplankton Photophysiology to Varying Environmental Conditions in the Sub-Antarctic and Polar Frontal Zone)
uri :
https://umexpert.um.edu.my/file/publication/00014562_159653_71191.pdf
Data also available via IMOS and AODN portal (IMOS and AODN portal)
uri :
https://portal.aodn.org.au/
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))
uri :
https://aesop.csiro.au/
Identifiers
- global : abd8b24a-7bea-4c97-b5f2-bf44ae727976
