Data

Southern Surveyor Voyage SS 01/95 HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) Data Officer (AR), Hobart (Processor of)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/abd8b24a-7bea-4c97-b5f2-bf44ae727976&rft.title=Southern Surveyor Voyage SS 01/95 HPLC Pigment and Ocean Colour Data&rft.identifier=abd8b24a-7bea-4c97-b5f2-bf44ae727976&rft.publisher=Integrated Marine Observing System&rft.description=This data was collected on Research Vessel Southern Surveyor, cruise SS 01/95. The voyage departed Hobart, Tasmania on the 14th of January, 1995 and returned to Hobart, Tasmania on the 12th of February, 1995. Samples were collected from 43 stations in order to characterise the optical properties of the Southern Ocean, south and west of Tasmania. Parameters measured include the phytoplankton pigment concentration and composition. The data can be used for Ocean Colour sensor validation.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=139.879; southlimit=-53.071; eastlimit=144.217; northlimit=-39.953&rft.coverage=westlimit=139.879; southlimit=-53.071; eastlimit=144.217; northlimit=-39.953&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=WfO Biogeochemistry&rft_subject=Climate Change Processes 1991-1995&rft_subject=Ship: Southern Surveyor&rft_subject=Research Voyage: SS 01/95&rft_subject=Niskin Bottles&rft_subject=Filtration Systems&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=States, Territories (Australia) | Tasmania&rft_subject=Global / Oceans | Southern Ocean&rft_subject=Marine Features (Australia) | Great Australian Bight, SA/WA&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Joint Global Ocean Flux Study, IGBP&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Attribution 4.0
https://creativecommons.org/licenses/by/4.0/

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

Access:

Open

Full description

This data was collected on Research Vessel Southern Surveyor, cruise SS 01/95. The voyage departed Hobart, Tasmania on the 14th of January, 1995 and returned to Hobart, Tasmania on the 12th of February, 1995. Samples were collected from 43 stations in order to characterise the optical properties of the Southern Ocean, south and west of Tasmania. Parameters measured include the phytoplankton pigment concentration and composition. The data can be used for Ocean Colour sensor validation.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).

Notes

Credit
Brian Griffiths
Credit
Research carried out on this cruise was partly supported by the Department of Environment, Sports and Territories, through the CSIRO Climate Change Research Program, and the Japanese Global Research Network System.

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Organisations

Related People

Related Services

144.217,-39.953 144.217,-53.071 139.879,-53.071 139.879,-39.953 144.217,-39.953

142.048,-46.512

text: westlimit=139.879; southlimit=-53.071; eastlimit=144.217; northlimit=-39.953

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Climate Change Processes 1991-1995 | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Ocean Color | Filtration Systems | Global / Oceans | Southern Ocean | HPLC (High Performance Liquid Chromatography) | Joint Global Ocean Flux Study, IGBP | Marine Features (Australia) | Great Australian Bight, SA/WA | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: SS 01/95 | Ship: Southern Surveyor | States, Territories (Australia) | Tasmania | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
SS 01/95 Survey details and related metadata, reports, events and data

uri : https://www.marine.csiro.au/data/trawler/survey_details.cfm?survey=SS199501

Cheah, W., McMinn, A., Griffiths, B., Westwood, K.J., Wright, S.W. & Clementson, L. (2013). Response of Phytoplankton Photophysiology to Varying Environmental Conditions in the Sub-Antarctic and Polar Frontal Zone. PloS one. 8. e72165. 10.1371/journal.pone.0072165. (Response of Phytoplankton Photophysiology to Varying Environmental Conditions in the Sub-Antarctic and Polar Frontal Zone)

uri : https://umexpert.um.edu.my/file/publication/00014562_159653_71191.pdf

Download the Pigment data (xlsx format) (Pigment Data)

uri : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=3976

Data also available via IMOS and AODN portal (IMOS and AODN portal)

uri : https://portal.aodn.org.au/

Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

uri : https://aesop.csiro.au/

Identifiers
  • global : abd8b24a-7bea-4c97-b5f2-bf44ae727976