Data

South-west Western Australia, April 2003, HPLC and Ocean Colour Data (AOCWG cruise)

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) Clementson, Lesley (Point of contact)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/ddbf0702-864a-2fe1-e043-08114f8cf492&rft.title=South-west Western Australia, April 2003, HPLC and Ocean Colour Data (AOCWG cruise)&rft.identifier=Anzlic Identifier: ANZCW0306014227&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected on the charter vessel Maritime Image between 14 and 17 April 2003 off the southwest of Western Australia . The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. Eleven stations were sampled. The data was used primarily to alidate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and absorption coefficient, dissolved and particulate. CLW deployed instruments RAMSES, AC-9, Hydroscat and Hydrorad.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the charter vessel Maritime Image. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 4-6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption: 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer.&rft.creator=Anonymous&rft.date=2013&rft.coverage=westlimit=115; southlimit=-32.6; eastlimit=116; northlimit=-31.5&rft.coverage=westlimit=115; southlimit=-32.6; eastlimit=116; northlimit=-31.5&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Absorption&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Coastal Waters (Australia) | West Australia Coast South, WA&rft_subject=Coastal Waters (Australia) | West Australia Coast West, WA&rft_subject=WfO Biogeochemistry&rft_subject=Remote Sensing 2000-2003&rft_subject=Ship: Maritime Image&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data collected on the charter vessel Maritime Image between 14 and 17 April 2003 off the southwest of Western Australia . The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. Eleven stations were sampled. The data was used primarily to alidate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and absorption coefficient, dissolved and particulate. CLW deployed instruments RAMSES, AC-9, Hydroscat and Hydrorad.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the charter vessel Maritime Image. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 4-6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption: 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer.

Notes

Credit
Ian Barton
Credit
Lesley Clementson
Credit
Kadija Oubelkheir(CLW)
Credit
Paul Daniel(CLW)
Credit
Peter Fearns (CMAR)

Data time period: 2003-04-14 to 2003-04-17

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116,-31.5 116,-32.6 115,-32.6 115,-31.5 116,-31.5

115.5,-32.05

text: westlimit=115; southlimit=-32.6; eastlimit=116; northlimit=-31.5

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | West Australia Coast South, WA | Coastal Waters (Australia) | West Australia Coast West, WA | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Absorption | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote Sensing 2000-2003 | Ship: Maritime Image | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)

url : https://aesop.csiro.au/

Download LC_IMOS_SRS_BioOptical_AOCWG_Apr_2003_TSS.zip (.csv, .nc, .xls) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2926

Download LC_IMOS_SRS_BioOptical_AOCWG_Apr_2003_absorption.zip (.csv, .nc, .xls) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2927

Download LC_IMOS_SRS_BioOptical_AOCWG_Apr_2003_pigments.zip (.csv, .nc, .xls) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2928

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

url : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • Local : Anzlic Identifier: ANZCW0306014227
  • Local : Marlin Record Number: 14227
  • global : ddbf0702-864a-2fe1-e043-08114f8cf492