Data

Sniper HPLC Pigment and Ocean Colour Data, August 1999 - January 2001

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/18222bd1-7495-43df-b95d-12ae58d34084&rft.title=Sniper HPLC Pigment and Ocean Colour Data, August 1999 - January 2001&rft.identifier=Anzlic Identifier: ANZCW0306006434&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected from 7 trips on the FV Sniper between 16 Aug 1999 and 8 Jan 2001. 88 samples were collected during these trips. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the FV Sniper. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 uL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, B,e-carotene and B,B-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=153; southlimit=-31; eastlimit=161; northlimit=-23&rft.coverage=westlimit=153; southlimit=-31; eastlimit=161; northlimit=-23&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Coastal Waters (Australia) | Queensland Coast South, QLD&rft_subject=Global / Oceans | Pacific Ocean&rft_subject=Regional Seas | Coral Sea&rft_subject=WfO Biogeochemistry&rft_subject=Ocean Colour 1997-2000&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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Brief description

This record contains data collected from 7 trips on the FV Sniper between 16 Aug 1999 and 8 Jan 2001. 88 samples were collected during these trips. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the FV Sniper. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis: 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 uL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, B,e-carotene and B,B-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.

Notes

Credit
Lesley Clementson
Credit
Jock Young
Credit
Clive Stanley
Credit
Pru Bonham

Data time period: 1999-08-16 to 2001-01-08

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161,-23 161,-31 153,-31 153,-23 161,-23

157,-27

text: westlimit=153; southlimit=-31; eastlimit=161; northlimit=-23

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | Queensland Coast South, QLD | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | Global / Oceans | Pacific Ocean | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Ocean Colour 1997-2000 | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Regional Seas | Coral Sea | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Identifiers
  • Local : Anzlic Identifier: ANZCW0306006434
  • Local : Marlin Record Number: 6434
  • global : 18222bd1-7495-43df-b95d-12ae58d34084
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