Data

Short Tailed Shearwater DREAM gene sequencing fastq files

Australian Antarctic Data Centre
DEAGLE, BRUCE E. ; DE PAOLI-ISEPPI, RICARDO ; POLANOWSKI, ANDREA
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5bd28a9be61d9&rft.title=Short Tailed Shearwater DREAM gene sequencing fastq files&rft.identifier=10.26179/5bd28a9be61d9&rft.publisher=Australian Antarctic Data Centre&rft.description=This data set includes unprocessed sample .fastq files from two separate Illumina NextSeq runs, labelled as 'Run_1' and 'Run_2', respectively. Sample names: e.g. STS15059, 'STS' is the abbreviation of Short-tailed shearwater. The first two digits of the numeric refer to the year of collection e.g. '15' = 2015. Finally, the following number refers to the sequential unique ID for that year, e.g. '059' is the fifty-ninth sample for the years' collection. Leg bands are also recorded and are generally a 5-digit number and are unique to the individual bird. Longitudinal samples can be identified using these band IDs. E.g. in Run_2, an individual with the band number: 52196, was collected in 2015 as 'STS15065' and again in 2017 as 'STS17044'. Run_1: N = 35 individual samples are split across 4 lanes e.g. 'STS16020_S35_L001(/L002/L003/L004)_R1_001/fastq' and need to be merged before conversion to .fasta format and downstream analysis. Run_2: N = 36 individual samples were provided as a single merged file from the service provider, e.g. 'STS15059_S34_R1_001.fastq'. Sample_info: This excel spreadsheet has information on samples as follows: 'Band': 5-digit number on leg band. 'Sample': Sample number within run. 'UID': The unique ID for collection year e.g. STS15007. 'Age': The known-age of the animal rounded to whole year. 'Index (NebNext)': The NEB index used for NGS sample identification. 'Note': Additional information on if a sample was a between or within run replicate or longitudinal replicate. Analysis of these data will be published in: [tba: R. De Paoli-Iseppi et al. 2018. Molecular Ecology Resources].&rft.creator=DEAGLE, BRUCE E. &rft.creator=DE PAOLI-ISEPPI, RICARDO &rft.creator=POLANOWSKI, ANDREA &rft.date=2018&rft.coverage=northlimit=-40.20825; southlimit=-40.31723; westlimit=148.08609; eastLimit=148.34579; projection=WGS84&rft.coverage=northlimit=-40.20825; southlimit=-40.31723; westlimit=148.08609; eastLimit=148.34579; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_shearwater_DREAM when using these data.&rft_subject=biota&rft_subject=oceans&rft_subject=ALBATROSSES/PETRELS AND ALLIES&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=ANIMALS/VERTEBRATES&rft_subject=BIRDS&rft_subject=DREAM&rft_subject=DNA&rft_subject=DNA METHYLATION&rft_subject=CHRONOLOGICAL AGE&rft_subject=SHORT-TAILED SHEARWATER&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Flinders Island&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_shearwater_DREAM when using these data.

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These data are publicly available, however owing to their size are only available on request to the Australian Antarctic Data Centre.

Brief description

This data set includes unprocessed sample .fastq files from two separate Illumina NextSeq runs, labelled as 'Run_1' and 'Run_2', respectively.

Sample names: e.g. STS15059, 'STS' is the abbreviation of Short-tailed shearwater. The first two digits of the numeric refer to the year of collection e.g. '15' = 2015. Finally, the following number refers to the sequential unique ID for that year, e.g. '059' is the fifty-ninth sample for the years' collection.

Leg bands are also recorded and are generally a 5-digit number and are unique to the individual bird. Longitudinal samples can be identified using these band IDs. E.g. in Run_2, an individual with the band number: 52196, was collected in 2015 as 'STS15065' and again in 2017 as 'STS17044'.

Run_1: N = 35 individual samples are split across 4 lanes e.g. 'STS16020_S35_L001(/L002/L003/L004)_R1_001/fastq' and need to be merged before conversion to .fasta format and downstream analysis.

Run_2: N = 36 individual samples were provided as a single merged file from the service provider, e.g. 'STS15059_S34_R1_001.fastq'.

Sample_info: This excel spreadsheet has information on samples as follows:
'Band': 5-digit number on leg band.
'Sample': Sample number within run.
'UID': The unique ID for collection year e.g. STS15007.
'Age': The known-age of the animal rounded to whole year.
'Index (NebNext)': The NEB index used for NGS sample identification.
'Note': Additional information on if a sample was a between or within run replicate or longitudinal replicate.

Analysis of these data will be published in: [tba: R. De Paoli-Iseppi et al. 2018. Molecular Ecology Resources].

Issued: 2018-10-18

Data time period: 2015-11-01 to 2017-12-31

This dataset is part of a larger collection

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148.34579,-40.20825 148.34579,-40.31723 148.08609,-40.31723 148.08609,-40.20825 148.34579,-40.20825

148.21594,-40.26274

text: northlimit=-40.20825; southlimit=-40.31723; westlimit=148.08609; eastLimit=148.34579; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | ALBATROSSES/PETRELS AND ALLIES | ANIMALS/VERTEBRATES | BIOLOGICAL CLASSIFICATION | BIRDS | CHRONOLOGICAL AGE | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Flinders Island | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania | DNA | DNA METHYLATION | DREAM | EARTH SCIENCE | GEOGRAPHIC REGION > POLAR | LABORATORY | OCEAN > SOUTHERN OCEAN | SHORT-TAILED SHEARWATER | biota | oceans |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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