Data

Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

Australian Antarctic Data Centre
CAVICCHIOLI, RICK ; HOFFMAN, JEFF ; MCQUAID, JEFF
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS&rft.title=Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses&rft.identifier=https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS&rft.publisher=Australian Antarctic Data Centre&rft.description=Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats. This work was completed as part of ASAC project 2899 (ASAC_2899).&rft.creator=CAVICCHIOLI, RICK &rft.creator=HOFFMAN, JEFF &rft.creator=MCQUAID, JEFF &rft.date=2008&rft.coverage=northlimit=-46.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84&rft.coverage=northlimit=-46.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS when using these data.&rft_subject=biota&rft_subject=oceans&rft_subject=PHYTOPLANKTON&rft_subject=EARTH SCIENCE&rft_subject=BIOSPHERE&rft_subject=AQUATIC ECOSYSTEMS&rft_subject=PLANKTON&rft_subject=phytoplankton&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=IPY > INTERNATIONAL POLAR YEAR&rft_subject=CASO > Climate of Antarctica and the Southern Ocean&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS when using these data.

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Brief description

Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.

This work was completed as part of ASAC project 2899 (ASAC_2899).

Issued: 2008-02-24

Data time period: 2007-12-17 to 2008-01-28

This dataset is part of a larger collection

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150,-46 150,-67 140,-67 140,-46 150,-46

145,-56.5

text: northlimit=-46.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84

Subjects
AQUATIC ECOSYSTEMS | BIOSPHERE | CASO > Climate of Antarctica and the Southern Ocean | EARTH SCIENCE | GEOGRAPHIC REGION > POLAR | IPY > INTERNATIONAL POLAR YEAR | OCEAN > SOUTHERN OCEAN | PHYTOPLANKTON | PLANKTON | R/V AA > R/V Aurora Australis | SHIPS | biota | oceans | phytoplankton |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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