Data

Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

Australian Ocean Data Network
Cavicchioli, R., Hoffman, J. and McQuaid, J. ; CAVICCHIOLI, RICK ; HOFFMAN, JEFF ; MCQUAID, JEFF
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS&rft.title=Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses&rft.identifier=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS&rft.publisher=Australian Antarctic Data Centre&rft.description=Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats. This work was completed as part of ASAC project 2899 (ASAC_2899).Progress Code: completedStatement: No known quality issues. GOS stands for Global Ocean Sampling. TFF- Tangential Flow Filtration.&rft.creator=Cavicchioli, R., Hoffman, J. and McQuaid, J. &rft.creator=CAVICCHIOLI, RICK &rft.creator=HOFFMAN, JEFF &rft.creator=MCQUAID, JEFF &rft.date=2008&rft.coverage=westlimit=140; southlimit=-67.0; eastlimit=150; northlimit=-46.0&rft.coverage=westlimit=140; southlimit=-67.0; eastlimit=150; northlimit=-46.0&rft_rights=This metadata record is publicly available.&rft_rights=These data are publicly available from the provided URL.&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS when using these data. http://creativecommons.org/licenses/by/4.0/).&rft_rights=Portable Network Graphic&rft_rights=https://i.creativecommons.org/l/by/3.0/88x31.png&rft_rights=Creative Commons by Attribution logo&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights=Legal code for Creative Commons by Attribution 4.0 International license&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON&rft_subject=phytoplankton&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=IPY > INTERNATIONAL POLAR YEAR&rft_subject=CASO > Climate of Antarctica and the Southern Ocean&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License
(http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS when using these data.
http://creativecommons.org/licenses/by/4.0/).

Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/legalcode

This metadata record is publicly available.

These data are publicly available from the provided URL.

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Creative Commons by Attribution logo

Attribution 4.0 International (CC BY 4.0)

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metadata@aad.gov.au

Brief description

Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.

This work was completed as part of ASAC project 2899 (ASAC_2899).

Lineage

Progress Code: completed
Statement: No known quality issues.

GOS stands for Global Ocean Sampling.
TFF- Tangential Flow Filtration.

Data time period: 2007-12-17 to 2008-01-28

150,-46 150,-67 140,-67 140,-46 150,-46

145,-56.5

text: westlimit=140; southlimit=-67.0; eastlimit=150; northlimit=-46.0

Subjects
AMD | AMD/AU | CASO > Climate of Antarctica and the Southern Ocean | CEOS | EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON | GEOGRAPHIC REGION > POLAR | IPY > INTERNATIONAL POLAR YEAR | OCEAN > SOUTHERN OCEAN | R/V AA > R/V Aurora Australis | SHIPS | biota | oceans | phytoplankton |

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Other Information
Download point for the data - excel spreadsheet (GET DATA)

uri : https://data.aad.gov.au/eds/1545/download

Genomic data obtained during V3 (2008). This is the primary data portal for genomic metadata, and stands for Community cyber-infrastructure for the Advancement of Microbial Ecology Research and Analysis (CAMERA). All genomic data obtained during this cruise will be curated and posted for public consumption at this site, in addition to being deposited in the major public genomic databases. (GET DATA)

uri : https://imicrobe.us/project/view/104

Genbank/NCBI website (GET DATA)

uri : http://www.ncbi.nlm.nih.gov/genbank/

Identifiers
  • global : CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS
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