Data

RV Investigator Voyage IN2016_V05 HPLC Pigment and Ocean Colour Data (September-October 2016)

Australian Ocean Data Network
CSIRO (Associated with) CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/fe55aa6b-0f1b-443b-814e-e2fc6cbc68be&rft.title=RV Investigator Voyage IN2016_V05 HPLC Pigment and Ocean Colour Data (September-October 2016)&rft.identifier=fe55aa6b-0f1b-443b-814e-e2fc6cbc68be&rft.publisher=Australian Ocean Data Network&rft.description=This record describes Bio-Optics data collected from the Marine National Facility (MNF) RV Investigator voyage: IN2016_V05, titled: The Great Barrier Reef as a significant source of climatically relevant aerosol particles. The data were collected as part of the supplementary project titled: Biogeochemical and optical properties of the Coral Sea and Queensland Shelf. The voyage, departing from and returning to Brisbane, QLD, took place between September 26 to October 24, 2016. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments, total suspended solids, and absorption coefficients.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=145.971; southlimit=-23.414; eastlimit=154.061; northlimit=-16.953000000000003; projection=WGS 84 (EPSG:4326)&rft.coverage=westlimit=145.971; southlimit=-23.414; eastlimit=154.061; northlimit=-16.953000000000003; projection=WGS 84 (EPSG:4326)&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=WfO Biogeochemistry&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Filtration Systems&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Marine Features (Australia) | Great Barrier Reef, QLD&rft_subject=Coastal Waters (Australia) | Queensland Coast, QLD&rft_subject=Regional Seas | Coral Sea&rft_subject=The Great Barrier Reef as a significant source of climatically relevant aerosol particles&rft_subject=eReefs&rft_subject=Research Voyage: IN2016_V05&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Attribution 4.0
https://creativecommons.org/licenses/by/4.0/

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

Access:

Other

Full description

This record describes Bio-Optics data collected from the Marine National Facility (MNF) RV Investigator voyage: IN2016_V05, titled: "The Great Barrier Reef as a significant source of climatically relevant aerosol particles." The data were collected as part of the supplementary project titled: "Biogeochemical and optical properties of the Coral Sea and Queensland Shelf." The voyage, departing from and returning to Brisbane, QLD, took place between September 26 to October 24, 2016. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments, total suspended solids, and absorption coefficients.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter was then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.

Notes

Credit
Janet Anstee (O&A Black Mountain)
Credit
Lesley Clementson (O&A Hobart)
Credit
Mark Baird (O&A Hobart)
Credit
Karen Wild-Allen (O&A Hobart)

Data time period: 2016-09-29 to 2016-10-22

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Data

Related People

154.061,-16.953 154.061,-23.414 145.971,-23.414 145.971,-16.953 154.061,-16.953

150.016,-20.1835

text: westlimit=145.971; southlimit=-23.414; eastlimit=154.061; northlimit=-16.953000000000003; projection=WGS 84 (EPSG:4326)

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Coastal Waters (Australia) | Queensland Coast, QLD | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Filtration Systems | Fluorometers | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Great Barrier Reef, QLD | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Regional Seas | Coral Sea | Research Voyage: IN2016_V05 | Spectrophotometers | Temperature of the water body | The Great Barrier Reef as a significant source of climatically relevant aerosol particles | WfO Biogeochemistry | eReefs | oceans | research vessel |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

url : https://aesop.csiro.au/

Download PFT_dataset_INV2016_v05_Bio-Optics.zip (IN2016_v05 bio-optics data [.xls])

url : https://www.marine.csiro.au/datacentre/projects/OceanColourData/RV_Investigator/PFT_dataset_INV2016_v05_Bio-Optics.zip

Data also available via IMOS and AODN portal (IMOS and AODN portal)

url : https://portal.aodn.org.au/

Identifiers
  • global : fe55aa6b-0f1b-443b-814e-e2fc6cbc68be