Data

RV Investigator Voyage IN2016_V03 HPLC pigment data (April-June 2016)

Integrated Marine Observing System
CSIRO (Associated with) CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/1c6f54b2-685c-4226-aea6-6373313e3f44&rft.title=RV Investigator Voyage IN2016_V03 HPLC pigment data (April-June 2016)&rft.identifier=1c6f54b2-685c-4226-aea6-6373313e3f44&rft.publisher=Integrated Marine Observing System&rft.description=This record describes HPLC pigment data collected from the Marine National Facility (MNF) RV Investigator voyage: IN2016_v03, titled Monitoring Ocean Change and Variability along 170 W from the ice edge to the equator. The voyage consisted of two legs: Leg-1 from Hobart (TAS) to Wellington (NZ) between April 27 and May 26, and Leg-2 from Wellington (NZ) to Lautoka (Fiji) from May 27 to June 28, 2016. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Samples for phytoplankton pigment analysis were collected using 2 x filtration racks and vacuum pumps (dimensions filtration rack: H:60cm, W:20cm; L: 50cm + 2 pumps H:20cm, W:20cm; L: 20cm). Samples for chlorophyll a were collected using one filtration pump (dimensions: H:60cm, W:60cm; L: 60 cm).Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. For pigment analysis, 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).&rft.creator=Anonymous&rft.date=2016&rft.coverage=westlimit=147; southlimit=-66; eastlimit=190; northlimit=0; projection=WGS 84 (EPSG:4326)&rft.coverage=westlimit=147; southlimit=-66; eastlimit=190; northlimit=0; projection=WGS 84 (EPSG:4326)&rft_rights=Attribution 4.0 https://creativecommons.org/licenses/by/4.0/&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Suspended Solids&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=WfO Biogeochemistry&rft_subject=Monitoring Ocean Change and Variability along 170W from the ice edge to the equator&rft_subject=Regional Seas | Tasman Sea&rft_subject=Countries | New Zealand&rft_subject=Countries | Fiji&rft_subject=Continents | Antarctica&rft_subject=Global / Oceans | Pacific Ocean&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Fluorometers&rft_subject=Filtration Systems&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Research Voyage: IN2016_V03&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Attribution 4.0
https://creativecommons.org/licenses/by/4.0/

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

Access:

Open

Full description

This record describes HPLC pigment data collected from the Marine National Facility (MNF) RV Investigator voyage: IN2016_v03, titled "Monitoring Ocean Change and Variability along 170 W from the ice edge to the equator." The voyage consisted of two legs: Leg-1 from Hobart (TAS) to Wellington (NZ) between April 27 and May 26, and Leg-2 from Wellington (NZ) to Lautoka (Fiji) from May 27 to June 28, 2016. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.

Samples for phytoplankton pigment analysis were collected using 2 x filtration racks and vacuum pumps (dimensions filtration rack: H:60cm, W:20cm; L: 50cm + 2 pumps H:20cm, W:20cm; L: 20cm). Samples for chlorophyll a were collected using one filtration pump (dimensions: H:60cm, W:60cm; L: 60 cm).

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. For pigment analysis, 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).

Notes

Credit
Bernadette Sloyan (O&A)
Credit
Bronte Tilbrook (O&A)
Credit
Susan Wijffels (O&A)
Credit
Lev Bodrossy (O&A)
Credit
Bec Cowley (O&A)
Credit
Mark Warner (University of Washington)
Credit
John Bullister (University of Washington)
Credit
Eric Raes (AWI)

Data time period: 2016-04-27 to 2016-06-28

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related Data

Related People

Spatial Coverage And Location

text: westlimit=147; southlimit=-66; eastlimit=190; northlimit=0; projection=WGS 84 (EPSG:4326)

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Continents | Antarctica | Countries | Fiji | Countries | New Zealand | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Chemistry | Suspended Solids | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Filtration Systems | Fluorometers | Global / Oceans | Pacific Ocean | HPLC (High Performance Liquid Chromatography) | Monitoring Ocean Change and Variability along 170W from the ice edge to the equator | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Regional Seas | Tasman Sea | Research Voyage: IN2016_V03 | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))

url : https://aesop.csiro.au/

Download LC_IMOS_SRS_BioOptical_IN2016_v03_pigments.xlsx (IN2016_v03 HPLC pigment data [.xlsx])

url : https://www.marine.csiro.au/datacentre/projects/OceanColourData/RV_Investigator/LC_IMOS_SRS_BioOptical_IN2016_v03 pigments.xlsx

Data also available via IMOS and AODN portal (IMOS and AODN portal)

url : https://portal.aodn.org.au/

Identifiers
  • global : 1c6f54b2-685c-4226-aea6-6373313e3f44