Brief description
This record describes HPLC pigment data collected from the Marine National Facility (MNF) RV Investigator voyage: IN2016_v03, titled "Monitoring Ocean Change and Variability along 170 W from the ice edge to the equator." The voyage consisted of two legs: Leg-1 from Hobart (TAS) to Wellington (NZ) between April 27 and May 26, and Leg-2 from Wellington (NZ) to Lautoka (Fiji) from May 27 to June 28, 2016. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Samples for phytoplankton pigment analysis were collected using 2 x filtration racks and vacuum pumps (dimensions filtration rack: H:60cm, W:20cm; L: 50cm + 2 pumps H:20cm, W:20cm; L: 20cm). Samples for chlorophyll a were collected using one filtration pump (dimensions: H:60cm, W:60cm; L: 60 cm).
Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. For pigment analysis, 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Notes
CreditBernadette Sloyan (O&A)
Credit
Bronte Tilbrook (O&A)
Bronte Tilbrook (O&A)
Credit
Susan Wijffels (O&A)
Susan Wijffels (O&A)
Credit
Lev Bodrossy (O&A)
Lev Bodrossy (O&A)
Credit
Bec Cowley (O&A)
Bec Cowley (O&A)
Credit
Mark Warner (University of Washington)
Mark Warner (University of Washington)
Credit
John Bullister (University of Washington)
John Bullister (University of Washington)
Credit
Eric Raes (AWI)
Eric Raes (AWI)
Data time period: 2016-04-27 to 2016-06-28
text: westlimit=147; southlimit=-66; eastlimit=-170; northlimit=0; projection=WGS 84 (EPSG:4326)
Subjects
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Continents | Antarctica |
Countries | Fiji |
Countries | New Zealand |
Earth Science | Oceans | Ocean Chemistry | Chlorophyll |
Earth Science | Oceans | Ocean Chemistry | Pigments |
Earth Science | Oceans | Ocean Chemistry | Suspended Solids |
Earth Science | Oceans | Ocean Optics | Ocean Color |
Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll |
Filtration Systems |
Fluorometers |
Global / Oceans | Pacific Ocean |
HPLC (High Performance Liquid Chromatography) |
Monitoring Ocean Change and Variability along 170W from the ice edge to the equator |
Niskin Bottles |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Regional Seas | Tasman Sea |
Research Voyage: IN2016_V03 |
Spectrophotometers |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))
uri :
https://aesop.csiro.au/
Download LC_IMOS_SRS_BioOptical_IN2016_v03_pigments.xlsx (IN2016_v03 HPLC pigment data [.xlsx])
Data also available via IMOS and AODN portal (IMOS and AODN portal)
Identifiers
- global : 1c6f54b2-685c-4226-aea6-6373313e3f44
