Data

RNA sequence data from krill CO2 exposure experiment

Australian Antarctic Division
Deagle, B. ; DEAGLE, BRUCE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
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Attribution 4.0 International (CC BY 4.0)
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These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA when using these data.

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We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm).

These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

Lineage

Progress Code: completed

Data time period: 2012-03-01 to 2012-09-30

This dataset is part of a larger collection

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147.29396,-42.9855 147.29396,-42.98792 147.29014,-42.98792 147.29014,-42.9855 147.29396,-42.9855

147.29205,-42.98671

text: westlimit=147.29014; southlimit=-42.98792; eastlimit=147.29396; northlimit=-42.9855

Subjects
ADS > Automated DNA Sequencer | AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) | EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE | GENETICS | GEOGRAPHIC REGION > POLAR | LABORATORY | OCEAN > SOUTHERN OCEAN | OCEAN ACIDIFICATION | RNA | biota | environment | oceans |

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Other Information
Download the dataset (GET DATA > DIRECT DOWNLOAD)

url : https://data.aad.gov.au/eds/4357/download

Public information for AAS project AAS_4015 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/report_project_public.cfm?project_no=AAS_4015

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

url : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA

Identifiers
ACN 633 798 857