Data

RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves

University of Tasmania, Australia
Wynn, Olivia
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25959/M4BS-SV29&rft.title=RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves&rft.identifier=10.25959/M4BS-SV29&rft.description=This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Bruny Island, Tasmania, and used in a controlled laboratory heatwave experiment. Individuals were exposed to ambient, single, or repeated marine heatwave treatments to assess the effects of thermal stress and recovery. Total RNA was extracted from blade tissue and sequenced on an Illumina platform to generate paired-end reads. The data represent high-quality raw FASTQ files (n = 30 samples; 5 replicates per treatment per time point) suitable for transcriptomic analyses examining the molecular responses of E. radiata to marine heatwaves.Maintenance and Update Frequency: notPlannedStatement: Juvenile Ecklonia radiata sporophytes were collected from Bruny Island, Tasmania, and acclimated in laboratory aquaria under controlled conditions. Individuals were exposed to three experimental temperature regimes simulating marine heatwave scenarios: no heatwave (15 °C throughout), a single 6-day heatwave at 20 °C, or two consecutive 6-day heatwaves at 20 °C separated by recovery periods at 15 °C. Samples for RNA sequencing were collected at two time points, during heatwave exposure and following recovery, from each treatment (n = 5 per treatment per time point). Blade tissue (approximately 50 mg) from each individual was preserved in RNA stabilization buffer and stored at –20 °C prior to extraction. Total RNA was extracted and purified before library preparation using the Illumina TruSeq Stranded mRNA Kit. Sequencing was performed by Macrogen Oceania (Sydney, Australia) on an Illumina NovaSeq X platform, generating 150 bp paired-end reads. This dataset contains the resulting raw FASTQ files for all samples.&rft.creator=Wynn, Olivia &rft.date=2025&rft_rights=This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).&rft_rights=Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/&rft_rights=Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/M4BS-SV29&rft_rights=Data, products and services from IMAS are provided as is without any warranty as to fitness for a particular purpose.&rft_rights=No known limitations.&rft_subject=biota&rft_subject=Laminariales&rft_subject=MACROALGAE (SEAWEEDS)&rft_subject=BROWN ALGAE&rft_subject=Coastal Waters (Australia) | Coastal Waters (Australia) | Tasmania Coast, TAS&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 4.0 International License
https://creativecommons.org/licenses/by/4.0/

This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).

Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to single and repeated marine heatwaves [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/M4BS-SV29

Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.

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This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Bruny Island, Tasmania, and used in a controlled laboratory heatwave experiment. Individuals were exposed to ambient, single, or repeated marine heatwave treatments to assess the effects of thermal stress and recovery. Total RNA was extracted from blade tissue and sequenced on an Illumina platform to generate paired-end reads. The data represent high-quality raw FASTQ files (n = 30 samples; 5 replicates per treatment per time point) suitable for transcriptomic analyses examining the molecular responses of E. radiata to marine heatwaves.

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Maintenance and Update Frequency: notPlanned
Statement: Juvenile Ecklonia radiata sporophytes were collected from Bruny Island, Tasmania, and acclimated in laboratory aquaria under controlled conditions. Individuals were exposed to three experimental temperature regimes simulating marine heatwave scenarios: no heatwave (15 °C throughout), a single 6-day heatwave at 20 °C, or two consecutive 6-day heatwaves at 20 °C separated by recovery periods at 15 °C. Samples for RNA sequencing were collected at two time points, during heatwave exposure and following recovery, from each treatment (n = 5 per treatment per time point). Blade tissue (approximately 50 mg) from each individual was preserved in RNA stabilization buffer and stored at –20 °C prior to extraction. Total RNA was extracted and purified before library preparation using the Illumina TruSeq Stranded mRNA Kit. Sequencing was performed by Macrogen Oceania (Sydney, Australia) on an Illumina NovaSeq X platform, generating 150 bp paired-end reads. This dataset contains the resulting raw FASTQ files for all samples.

Notes

Credit
This dataset was generated as part of Olivia J. Wynn’s PhD research at the Institute for Marine and Antarctic Studies (University of Tasmania) under the supervision of Professor Catriona L. Hurd, Dr. Damon Britton, and Dr. Cintia Iha, with collaborators Professor John Beardall and Assistant Professor Juan-Diego Gaitán-Espitia. This research was supported by the Australian Research Council (ARC) Discovery Project DP200101467, awarded to Professor Catriona L. Hurd.

Issued: 19 11 2025

Data time period: 2024-07-30 to 2025-02-19

This dataset is part of a larger collection

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Subjects
BROWN ALGAE | Coastal Waters (Australia) | Coastal Waters (Australia) | Tasmania Coast, TAS | Laminariales | MACROALGAE (SEAWEEDS) | biota |

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url : https://data.imas.utas.edu.au/attachments/a9fc13db-1292-4e8f-a5f0-cde07af6ccd8/

local : 00arpt780

local : 0000-0003-0417-6938

local : 00arpt780

local : 0000-0003-0417-6938

Identifiers
ACN 633 798 857