Data

RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments

University of Tasmania, Australia
Wynn, Olivia
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25959/QZXM-VV84&rft.title=RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments&rft.identifier=10.25959/QZXM-VV84&rft.description=This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Coal Point, Tasmania, and used in a controlled laboratory experiment examining the effects of temperature and elevated CO₂ on gene expression. Individuals were exposed to three temperatures (6, 16, and 26 °C) under two CO₂ levels: current (~420 µatm) and future (~1000 µatm), representing RCP 8.5 projections. Total RNA was extracted from blade tissue and sequenced on an Illumina NovaSeq 6000 platform to produce 150 bp paired-end reads. The dataset includes raw FASTQ files from 30 biological samples (n = 5 per treatment combination) used for transcriptomic analyses investigating the molecular responses of E. radiata to ocean warming and acidification.Maintenance and Update Frequency: notPlannedStatement: Juvenile Ecklonia radiata were collected from Coal Point, Tasmania, and acclimated under controlled laboratory conditions. Individuals were exposed to a factorial design of three temperatures (6, 16, 26 °C) and two CO₂ levels (~420 µatm and ~1000 µatm) for 21 days. Blade tissue (~50 mg) was preserved in RNA stabilization buffer and stored at −20 °C prior to extraction. Total RNA was extracted, purified, and prepared for sequencing using the Illumina TruSeq RNA Library Prep Kit with poly(A) enrichment. Sequencing was performed by Macrogen Inc. (Seoul, South Korea) on an Illumina NovaSeq 6000, generating 150 bp paired-end reads. This dataset includes the resulting raw FASTQ files for all samples.&rft.creator=Wynn, Olivia &rft.date=2025&rft_rights=This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).&rft_rights=Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/&rft_rights=Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/QZXM-VV84&rft_rights=Data, products and services from IMAS are provided as is without any warranty as to fitness for a particular purpose.&rft_rights=No known limitations.&rft_subject=biota&rft_subject=Laminariales&rft_subject=MACROALGAE (SEAWEEDS)&rft_subject=BROWN ALGAE&rft_subject=Coastal Waters (Australia) | Coastal Waters (Australia) | Tasmania Coast, TAS&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 4.0 International License
https://creativecommons.org/licenses/by/4.0/

This dataset is the intellectual property of the University of Tasmania (UTAS) through the Institute for Marine and Antarctic Studies (IMAS).

Cite data as: Wynn, O. (2025). RNA-seq data from Ecklonia radiata exposed to combined temperature and CO₂ treatments [Data set]. Institute for Marine and Antarctic Studies. https://doi.org/10.25959/QZXM-VV84

Data, products and services from IMAS are provided "as is" without any warranty as to fitness for a particular purpose.

No known limitations.

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This dataset contains raw RNA sequencing reads from juvenile Ecklonia radiata (Laminariales) collected from Coal Point, Tasmania, and used in a controlled laboratory experiment examining the effects of temperature and elevated CO₂ on gene expression. Individuals were exposed to three temperatures (6, 16, and 26 °C) under two CO₂ levels: current (~420 µatm) and future (~1000 µatm), representing RCP 8.5 projections. Total RNA was extracted from blade tissue and sequenced on an Illumina NovaSeq 6000 platform to produce 150 bp paired-end reads. The dataset includes raw FASTQ files from 30 biological samples (n = 5 per treatment combination) used for transcriptomic analyses investigating the molecular responses of E. radiata to ocean warming and acidification.

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Maintenance and Update Frequency: notPlanned
Statement: Juvenile Ecklonia radiata were collected from Coal Point, Tasmania, and acclimated under controlled laboratory conditions. Individuals were exposed to a factorial design of three temperatures (6, 16, 26 °C) and two CO₂ levels (~420 µatm and ~1000 µatm) for 21 days. Blade tissue (~50 mg) was preserved in RNA stabilization buffer and stored at −20 °C prior to extraction. Total RNA was extracted, purified, and prepared for sequencing using the Illumina TruSeq RNA Library Prep Kit with poly(A) enrichment. Sequencing was performed by Macrogen Inc. (Seoul, South Korea) on an Illumina NovaSeq 6000, generating 150 bp paired-end reads. This dataset includes the resulting raw FASTQ files for all samples.

Notes

Credit
This dataset was generated as part of Olivia J. Wynn’s PhD research at the Institute for Marine and Antarctic Studies (University of Tasmania) under the supervision of Professor Catriona L. Hurd, Dr Damon Britton, and Dr Cintia Iha, with collaborators Professor John Beardall and Assistant Professor Juan-Diego Gaitán-Espitia. This research was supported by the Australian Research Council (ARC) Discovery Project DP200101467, awarded to Professor Catriona L. Hurd.

Issued: 19 11 2025

Data time period: 2021-02-22 to 2022-05-17

This dataset is part of a larger collection

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Subjects
BROWN ALGAE | Coastal Waters (Australia) | Coastal Waters (Australia) | Tasmania Coast, TAS | Laminariales | MACROALGAE (SEAWEEDS) | biota |

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url : https://data.imas.utas.edu.au/attachments/8a3b5597-32d4-4b9d-9284-939b6197ded5/

local : 00arpt780

local : 0000-0003-0417-6938

local : 00arpt780

local : 0000-0003-0417-6938

Identifiers
ACN 633 798 857