Data

Reef location has a greater impact than coral bleaching severity on the microbiome of Pocillopora acuta

Australian Ocean Data Network
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=0ff3a65f-6ee9-4448-bfff-8ae3c86b6115&rft.title=Reef location has a greater impact than coral bleaching severity on the microbiome of Pocillopora acuta&rft.identifier=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=0ff3a65f-6ee9-4448-bfff-8ae3c86b6115&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=The composition of the microbial communities in the coral Pocillopora acuta were examined following the 2016 bleaching event on the Great Barrier Reef (GBR). Corals located within two reefs of the Palm Island archipelago on the Central GBR experienced moderate heat stress, In-water bleaching surveys were conducted at two depths (2 m and 5 m) on the 4th and 6th of April 2016. Five 10 × 1 m belt transects were used with observers capturing 1 m × 1 m square continuous images. Observers counted each coral colony > 5 cm in diameter within each 1 m × 1 m image, recorded: genus level identification categorical bleaching score based upon the proportion of living coral tissue visibly bleached white: No bleaching Pale 1–50% tissue bleached white 51–99% bleached white 100% bleached white with or without intense fluorescence partial or complete mortality of the colony (recently dead regions of tissue). Bleaching severity categories at the reef community level were based on the percentage of corals bleached as follows: no bleaching (0% bleached corals); minor bleaching (1–10%), moderate (10–30%); major (30–60%); severe (> 60%) Coral fragments were collected from 22 colonies at Pandora on the 4th April 2016 and from 25 colonies at Havannah on the 12th April 2016 Samples were prepared for DNA extraction and Symbiodiniaceae cell counts as described in (Botte et al. 2021). Surface area of the coral skeletons was estimated using established protocol (Veal et al. 2010). Statistical analysis of DNA sequencing data was conducted using R. This study was conducted to identify the impact of bleaching severity on the microbiome of the P. acuta coral during the mass bleaching event of summer 2016, at two sampling sites within the Palm Island group (Central GBR). A significant impact of sampling site was found and no effect of bleaching severity, demonstrating the importance of fine-scale sampling when investigating large scale bleaching events.Maintenance and Update Frequency: notPlannedStatement: Survey methods used to assess reef community bleaching severity aligned with and contributed to the 2016 Australian National Bleaching Taskforce GBR Survey response (Hughes et al. 2017) Hughes TP, Kerry JT, Álvarez-Noriega M, Álvarez-Romero JG, Anderson KD, Baird AH, Babcock RC, Beger M, Bellwood DR, Berkelmans R, Bridge TC, Butler IR, Byrne M, Cantin NE, Comeau S, Connolly SR, Cumming GS, Dalton SJ, Guillermo Diaz-Pulido C, Eakin M, Figueira WF, Gilmour JP, Harrison HB, Heron SF, Hoey AS, Hobbs J-PA, Hoogenboom MO, Kennedy EV, Kuo C-Y, Lough JM, Lowe RJ, Liu G, McCulloch MT, Malcolm HA, McWilliam MJ, Pandolfi JM, Pears RJ, Pratchett MS, Schoepf V, Simpson T, Skirving WJ, Sommer B, Torda G, Wachenfeld DR, Willis BL, Wilson SK (2017) Global warming and recurrent mass bleaching of corals. Nature 543:373–377 https://doi.org/10.1038/nature21707 Wax dipping technique for surface area estimation protocols: Veal CJ, Holmes G, Nunez M, Hoegh-Guldberg O, Osborn J (2010) A comparative study of methods for surface area and three-dimensional shape measurement of coral skeletons. Limnol Oceanogr Meth 8:241–253&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=147.4313056; southlimit=-18.81525; eastlimit=147.4313056; northlimit=-18.81525&rft.coverage=westlimit=147.4313056; southlimit=-18.81525; eastlimit=147.4313056; northlimit=-18.81525&rft.coverage=westlimit=146.5339167; southlimit=-18.83961111; eastlimit=146.5339167; northlimit=-18.83961111&rft.coverage=westlimit=146.5339167; southlimit=-18.83961111; eastlimit=146.5339167; northlimit=-18.83961111&rft_rights= http://creativecommons.org/licenses/by/3.0/au/&rft_rights=http://i.creativecommons.org/l/by/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2022). Reef location has a greater impact than coral bleaching severity on the microbiome of Pocillopora acuta. https://apps.aims.gov.au/metadata/view/0ff3a65f-6ee9-4448-bfff-8ae3c86b6115, accessed[date-of-access].&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2022). Reef location has a greater impact than coral bleaching severity on the microbiome of Pocillopora acuta. https://apps.aims.gov.au/metadata/view/0ff3a65f-6ee9-4448-bfff-8ae3c86b6115, accessed[date-of-access]".

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Brief description

The composition of the microbial communities in the coral Pocillopora acuta were examined following the 2016 bleaching event on the Great Barrier Reef (GBR). Corals located within two reefs of the Palm Island archipelago on the Central GBR experienced moderate heat stress,


In-water bleaching surveys were conducted at two depths (2 m and 5 m) on the 4th and 6th of April 2016. Five 10 × 1 m belt transects were used with observers capturing 1 m × 1 m square continuous images. Observers counted each coral colony > 5 cm in diameter within each 1 m × 1 m image, recorded:


genus level identification categorical bleaching score based upon the proportion of living coral tissue visibly bleached white:



No bleaching

Pale

1–50% tissue bleached white

51–99% bleached white

100% bleached white with or without intense fluorescence

partial or complete mortality of the colony (recently dead regions of tissue).



Bleaching severity categories at the reef community level were based on the percentage of corals bleached as follows:



no bleaching (0% bleached corals);

minor bleaching (1–10%),

moderate (10–30%);

major (30–60%);

severe (> 60%)



Coral fragments were collected from 22 colonies at Pandora on the 4th April 2016 and from 25 colonies at Havannah on the 12th April 2016


Samples were prepared for DNA extraction and Symbiodiniaceae cell counts as described in (Botte et al. 2021). Surface area of the coral skeletons was estimated using established protocol (Veal et al. 2010). Statistical analysis of DNA sequencing data was conducted using R.


This study was conducted to identify the impact of bleaching severity on the microbiome of the P. acuta coral during the mass bleaching event of summer 2016, at two sampling sites within the Palm Island group (Central GBR). A significant impact of sampling site was found and no effect of bleaching severity, demonstrating the importance of fine-scale sampling when investigating large scale bleaching events.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Survey methods used to assess reef community bleaching severity aligned with and contributed to the 2016 Australian National Bleaching Taskforce GBR Survey response (Hughes et al. 2017)


Hughes TP, Kerry JT, Álvarez-Noriega M, Álvarez-Romero JG, Anderson KD, Baird AH, Babcock RC, Beger M, Bellwood DR, Berkelmans R, Bridge TC, Butler IR, Byrne M, Cantin NE, Comeau S, Connolly SR, Cumming GS, Dalton SJ, Guillermo Diaz-Pulido C, Eakin M, Figueira WF, Gilmour JP, Harrison HB, Heron SF, Hoey AS, Hobbs J-PA, Hoogenboom MO, Kennedy EV, Kuo C-Y, Lough JM, Lowe RJ, Liu G, McCulloch MT, Malcolm HA, McWilliam MJ, Pandolfi JM, Pears RJ, Pratchett MS, Schoepf V, Simpson T, Skirving WJ, Sommer B, Torda G, Wachenfeld DR, Willis BL, Wilson SK (2017) Global warming and recurrent mass bleaching of corals. Nature 543:373–377 https://doi.org/10.1038/nature21707


Wax dipping technique for surface area estimation protocols: Veal CJ, Holmes G, Nunez M, Hoegh-Guldberg O, Osborn J (2010) A comparative study of methods for surface area and three-dimensional shape measurement of coral skeletons. Limnol Oceanogr Meth 8:241–253

Notes

Credit
Cantin, N. (AIMS)
Credit
O'Brien, PA. (AIMS) and James Cook University (JCU) and AIMS@JCU
Credit
Webster, NS. (AIMS), Australian Antarctic Division, University of Queensland (UQ)
Credit
Botté, ES. Australian Institute of Marine Science (AIMS) and University of New South Wales (UNSW)
Credit
Frade, PR. Natural History Museum Vienna, Vienna, Austria
Credit
Moecellin, VL. (AIMS)
Credit
Rocker, MM. Deakin University, Victoria

Modified: 03 2024

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147.43131,-18.81525

147.4313056,-18.81525

146.53392,-18.83961

146.5339167,-18.83961111

text: westlimit=147.4313056; southlimit=-18.81525; eastlimit=147.4313056; northlimit=-18.81525

text: westlimit=146.5339167; southlimit=-18.83961111; eastlimit=146.5339167; northlimit=-18.83961111

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Other Information
Sea Water Temperature Logger Data At Pandora Reef, From 04 Jul 2007 To 04 Jun 2019

uri : https://apps.aims.gov.au/metadata/view/8E75CB5F-62AD-1BA7-E054-0010E056919A

Microbiome of reef-building coral Pocillopora acuta on the Great Barrier Reef of Australia (Havannah Island and Pandora reefs) during coral bleaching in 2016 - NCBI Sequence Reads Archive (https://www.ncbi.nlm.nih.gov/sra) under submission PRJNA743719

uri : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA743719/

Sea Water Temperature Logger Data At Havannah Island, From 03 Jul 2007 To 13 May 2021

uri : https://apps.aims.gov.au/metadata/view/871c8762-45a6-41f6-9bd5-1c05b1fcfd89

Identifiers
  • global : 0ff3a65f-6ee9-4448-bfff-8ae3c86b6115
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