Data

Recombinant mouse CRISP protein add-back experiments

Monash University
Avinash Gaikwad (Aggregated by) Moira O'Bryan (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26180/5dbf4bff1acce&rft.title=Recombinant mouse CRISP protein add-back experiments&rft.identifier=https://doi.org/10.26180/5dbf4bff1acce&rft.publisher=Monash University&rft.description=I. Representative sperm from Crisp1, and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa.Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps.&rft.creator=Avinash Gaikwad&rft.creator=Avinash Gaikwad&rft.creator=Moira O'Bryan&rft.creator=Moira O'Bryan&rft.date=2019&rft_rights=CC-BY-4.0&rft_subject=male fertility&rft_subject=male infertility&rft_subject=CRISP1&rft_subject=CRISP2&rft_subject=CRISP4&rft_subject=CRISP1/4&rft_subject=sperm&rft_subject=sperm function&rft_subject=reproduction&rft_subject=Genetics&rft_subject=Molecular Biology&rft.type=dataset&rft.language=English Access the data
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I. Representative sperm from Crisp1, and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins.

II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa.

Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps.

Issued: 2019-11-03

Created: 2019-11-03

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CRISP1 | CRISP1/4 | CRISP2 | CRISP4 | Genetics | Molecular Biology | male fertility | male infertility | reproduction | sperm | sperm function |

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