Data

Real-time PCR measurements of alkB in SAB contaminated sediment (SRE4 experiment).

Australian Antarctic Division
Bowman, J. and Powell, S. ; BOWMAN, JOHN ; POWELL, SHANE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5d3e7b2896119&rft.title=Real-time PCR measurements of alkB in SAB contaminated sediment (SRE4 experiment).&rft.identifier=10.26179/5d3e7b2896119&rft.publisher=Australian Antarctic Data Centre&rft.description=Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by using a real-time PCR assay to measure the levels of alkane mono-oxygenase genes (alkB). In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. The data in this record is for all time points in the experiment.The download file contains a readme which provides further information about the dataset, as well as an excel and csv copy of the data. This work was completed as part of ASAC project 2672 (ASAC_2672).Progress Code: completedStatement: The dates provided in temporal coverage are a range. The specific dates of sample collection are given in the datafile.&rft.creator=Bowman, J. and Powell, S. &rft.creator=BOWMAN, JOHN &rft.creator=POWELL, SHANE &rft.date=2008&rft.coverage=westlimit=110.18457; southlimit=-66.60073; eastlimit=110.68457; northlimit=-66.10073&rft.coverage=westlimit=110.18457; southlimit=-66.60073; eastlimit=110.68457; northlimit=-66.10073&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_allyears when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=environment&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS&rft_subject=quantitative real-time PCR&rft_subject=alkane mono-oxygenase&rft_subject=special Antarctic blend diesel&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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CC-BY

Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_allyears when using these data.

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Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by using a real-time PCR assay to measure the levels of alkane mono-oxygenase genes (alkB). In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment.

The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. The data in this record is for all time points in the experiment.
The download file contains a readme which provides further information about the dataset, as well as an excel and csv copy of the data.

This work was completed as part of ASAC project 2672 (ASAC_2672).

Lineage

Progress Code: completed
Statement: The dates provided in temporal coverage are a range. The specific dates of sample collection are given in the datafile.

Data time period: 2001-10-01 to 2006-12-20

This dataset is part of a larger collection

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110.68457,-66.10073 110.68457,-66.60073 110.18457,-66.60073 110.18457,-66.10073 110.68457,-66.10073

110.43457,-66.35073

text: westlimit=110.18457; southlimit=-66.60073; eastlimit=110.68457; northlimit=-66.10073

Subjects
ADS > Automated DNA Sequencer | AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA | EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS | GEOGRAPHIC REGION > POLAR | LABORATORY | alkane mono-oxygenase | environment | quantitative real-time PCR | special Antarctic blend diesel |

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Other Information
Download point for the dataset (GET DATA)

url : https://data.aad.gov.au/eds/2975/download

Public information for ASAC project 1228 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=1228

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_allyears

Identifiers
ACN 633 798 857