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Raw spectral data - Alterations in membrane lipid content and lipid biosynthesis pathways in plasma membrane and tonoplast in response to salt stress

Southern Cross University
Guo, Qi ; Liu, Ben ; Rupasinghe, Thusitha ; Roessner, Ute ; Barkla, Bronwyn
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25918/data.168&rft.title=Raw spectral data - Alterations in membrane lipid content and lipid biosynthesis pathways in plasma membrane and tonoplast in response to salt stress&rft.identifier=10.25918/data.168&rft.publisher=Southern Cross University&rft.description=Membranes of plant cells are the sites of sensing and initiation of rapid responses to changing environmental factors including under salinity stress. Understanding the mechanisms involved in membrane remodelling is of great significance to studying salt tolerance in plants. This task remains challenging in complex tissue due to suboptimal subcellular membrane isolation techniques. Here, we capitalized on the use of a surface charge-based separation method - Free Flow Electrophoresis to isolate tonoplast and plasma membrane from leaf tissue of the halophyte Mesembryanthemum crystallinum. Results demonstrated a membrane-specific lipidomic remodelling in this plant under salt conditions, including an increased proportion of bilayer forming lipid phosphatidylcholine in the tonoplast and an increase in non-bilayer forming and negatively charged lipids (phosphatidylethanolamine and phosphatidylserine) in the plasma membrane. Quantitative proteomics showed salt-induced changes in proteins involved in fatty acid synthesis and desaturation, glycerolipid and sterol synthesis, as well as proteins involved in lipid signalling, binding, and trafficking. These results reveal an essential plant mechanism for membrane homeostasis wherein lipidome remodelling in response to salt stress contributes to maintaining the physiological function of individual subcellular compartments&rft.creator=Guo, Qi &rft.creator=Liu, Ben &rft.creator=Rupasinghe, Thusitha &rft.creator=Roessner, Ute &rft.creator=Barkla, Bronwyn &rft.date=2021&rft_rights=CC BY V4.0&rft_subject=Proteomics&rft_subject=Lipidomics&rft_subject=Salt stress&rft_subject=Cell membrane&rft_subject=Halophyte&rft_subject=Proteomics and Intermolecular Interactions (excl. Medical Proteomics)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=BIOCHEMISTRY AND CELL BIOLOGY&rft_subject=Receptors and Membrane Biology&rft.type=dataset&rft.language=English Access the data

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Membranes of plant cells are the sites of sensing and initiation of rapid responses to changing environmental factors including under salinity stress. Understanding the mechanisms involved in membrane remodelling is of great significance to studying salt tolerance in plants. This task remains challenging in complex tissue due to suboptimal subcellular membrane isolation techniques. Here, we capitalized on the use of a surface charge-based separation method - Free Flow Electrophoresis to isolate tonoplast and plasma membrane from leaf tissue of the halophyte Mesembryanthemum crystallinum. Results demonstrated a membrane-specific lipidomic remodelling in this plant under salt conditions, including an increased proportion of bilayer forming lipid phosphatidylcholine in the tonoplast and an increase in non-bilayer forming and negatively charged lipids (phosphatidylethanolamine and phosphatidylserine) in the plasma membrane. Quantitative proteomics showed salt-induced changes in proteins involved in fatty acid synthesis and desaturation, glycerolipid and sterol synthesis, as well as proteins involved in lipid signalling, binding, and trafficking. These results reveal an essential plant mechanism for membrane homeostasis wherein lipidome remodelling in response to salt stress contributes to maintaining the physiological function of individual subcellular compartments

Issued: 20211031

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