Data

Raw mass spec data of PRC2-PALI1PIR-long complex in the presence or absence of SAM

Monash University
Qi Zhang (Aggregated by)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26180/14752509&rft.title=Raw mass spec data of PRC2-PALI1PIR-long complex in the presence or absence of SAM&rft.identifier=https://doi.org/10.26180/14752509&rft.publisher=Monash University&rft.description=For the identification of methylations in the recombinant PRC2-PALI1PIR-long complex, 0.5 µM protein was incubated in the presence or absence of 20 µM SAM (NEB #B9003) in 25 mM HEPES pH 8.0, 50 mM NaCl, 2 mM MgCl2, 2 mM 2-mercaptoethanol and 10 % glycerol for 1 hour at 30 °C, in a total final volume of 85 µL. The proteins were then alkylated, subjected to tryptic digestion and the tryptic peptides where treated and analysed by MS/MS.&rft.creator=Qi Zhang&rft.date=2021&rft_rights=CC-BY-4.0&rft_subject=raw data&rft_subject=LC-MS/MS&rft_subject=Methylation&rft_subject=Proteomics and Intermolecular Interactions (excl. Medical Proteomics)&rft_subject=Biochemistry and Cell Biology not elsewhere classified&rft.type=dataset&rft.language=English Access the data

Licence & Rights:

Open Licence view details
CC-BY

CC-BY-4.0

Full description

For the identification of methylations in the recombinant PRC2-PALI1PIR-long complex, 0.5 µM protein was incubated in the presence or absence of 20 µM SAM (NEB #B9003) in 25 mM HEPES pH 8.0, 50 mM NaCl, 2 mM MgCl2, 2 mM 2-mercaptoethanol and 10 % glycerol for 1 hour at 30 °C, in a total final volume of 85 µL. The proteins were then alkylated, subjected to tryptic digestion and the tryptic peptides where treated and analysed by MS/MS.

Issued: 2021-06-09

Created: 2021-06-09

Click to explore relationships graph
Subjects

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information