Ral-mediated signalling pathways in neurons [ 2001 - 2003 ]

Research Grant

Researchers: Prof Basil Roufogalis (Principal investigator) ,  Prof Phillip Robinson

Brief description Within the nervous system, neurons communicate through the release of neurotransmitter chemicals across connections between individual neurons (synapses). Before their release, neurotransmitters are stored inside nerve endings, within small membranous spheres called synaptic vesicles. Neuronal cell shape and the neuron's ability to migrate to different regions of the brain during development affect the way that the adult brain functions. Alterations in any of these brain functions may lead to diseases affecting normal mental function. Ral is a small GTPase enzyme found in brain, and particularly in neurons. Small GTPases are responsible for regulating cell functions by acting as switches, turning biochemical processes on and off inside the cell. Within neurons, Ral is found on the surface of synaptic vesicles, implicating it in the regulation of neurotransmitter release. Other Ral functions have been demonstrated in non-neuronal cells that may be of particular significance in neuronal cells. However, no studies have previously investigated Ral function in the nervous system. The research proposed aims to establish what role RalA performs within neuronal cells, and by what biochemical mechanism it performs this role. Techniques of molecular biology, biochemistry and microscopy will be used to establish these functions. This research will lead to increased knowledge of the significance of this protein to cellular, and particularly neuronal cell function. This forms the basis for the understanding normal neuronal function, and for the identification of factors causing diseases of the nervous system. In time, such research aids in the development of specific therapies for sufferers of such diseases of the nervous system.

Funding Amount $AUD 317,545.90

Funding Scheme NHMRC Project Grants

Notes Standard Project Grant