Data

Population genetics of the mangrove Rhizophora stylosa in northern Australia

Australian Ocean Data Network
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=31caee9f-a376-410a-b53b-419d5a37c694&rft.title=Population genetics of the mangrove Rhizophora stylosa in northern Australia&rft.identifier=http://catalogue-aodn.prod.aodn.org.au/geonetwork/srv/eng/search?uuid=31caee9f-a376-410a-b53b-419d5a37c694&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Starch gel electrophoresis was used to examine genetic variation in five widely separated populations of the mangrove Rhizophora stylosa Griff. Shoots containing leaves and well developed apical buds were collected between March and August 1987 from Darwin, the Daintree River, 2 sites at Missionary Bay (Hinchinbrook Island), Chunda Bay (Cape Ferguson) and Lardelli Creek (Ayr). No two trees at each sampling site were separated by more than 1km, except for the Daintree River site, which was sampled over 2km.A suitable extraction buffer was developed by modifying a buffer developed by Dr Gavin Moran (CSIRO) for eucalypts and acacias.Twenty eight enzymes were investigated for the Rhizophora stylosa samples (ACP, ACON, AK, ADH, ALD, AAT, EST(FL), FH, BetaGAL, G6PD, GPI, GDH, GSR, GAPDH, HK, IDH, LAP, LGGP, LTP, LPP, MDH, ME, MDR, PER, PGM, 6PGD, SKDH, TPI). Fourteen enzymes were resolved adequately for these samples (ACON, AAT, GPI, GDH, GSR, HK, IDH, LAP, LTP, MDH, ME, PER, 6PGD, TPI).Other Rhizophoraceae successfully tested for enzyme activity in leaf and apical bud tissue, using the same methods were Rhizophora apiculata Blume, R. lamarckii Montr., R. mucronata Lamarck, Bruguiera gymnorhiza Lamarck, Ceriops decandra (Griff.) Ding Hou, C. tagal (Perr.) C.B. Rob. var. tagal and C. tagal var. australis C.T. White. Other mangrove species producing clear repeatable results included Lumnitzera racemosa Willdenow, Xylocarpus australasicus Ridley, Sonneratia alba J.E. Smith, Acrostichum speciosum Willdenow, Avicennia marina (Forssk.) Vierh. and A. officinalis Linnaeus.While leaves and buds were chosen initially, due to their year round availability, whole anthers and the plumule of the progagule of Rhizophora stylosa were also successfully tested for enzyme activity. This research was undertaken to:1. develop an effective extraction buffer to counteract the effect of complex secondary metabolites in mangroves, which denature proteins during enzyme extraction.2. develop techniques for the electrophoresis of mangrove tissue and to use these to investigate genetic variability in Rhizophora stylosa and other Rhizophoraceae.Maintenance and Update Frequency: notPlannedStatement: Statement: The techniques used for enzyme extraction and electrophoresis are described in the following paper:Goodall JA and Stoddart JA (1989) Techniques for the electrophoresis of mangrove tissue. Aquatic Botany 35: 197-207.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=130.833333; southlimit=-12.416667; eastlimit=130.833333; northlimit=-12.416667&rft.coverage=westlimit=130.833333; southlimit=-12.416667; eastlimit=130.833333; northlimit=-12.416667&rft.coverage=westlimit=145.483333; southlimit=-16.3; eastlimit=145.483333; northlimit=-16.3&rft.coverage=westlimit=145.483333; southlimit=-16.3; eastlimit=145.483333; northlimit=-16.3&rft.coverage=westlimit=146.2; southlimit=-18.25; eastlimit=146.2; northlimit=-18.25&rft.coverage=westlimit=146.2; southlimit=-18.25; eastlimit=146.2; northlimit=-18.25&rft.coverage=westlimit=147.05; southlimit=-19.266667; eastlimit=147.05; northlimit=-19.266667&rft.coverage=westlimit=147.05; southlimit=-19.266667; eastlimit=147.05; northlimit=-19.266667&rft.coverage=westlimit=147.583333; southlimit=-19.583333; eastlimit=147.583333; northlimit=-19.583333&rft.coverage=westlimit=147.583333; southlimit=-19.583333; eastlimit=147.583333; northlimit=-19.583333&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2010). Population genetics of the mangrove Rhizophora stylosa in northern Australia. https://apps.aims.gov.au/metadata/view/31caee9f-a376-410a-b53b-419d5a37c694, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2010). Population genetics of the mangrove Rhizophora stylosa in northern Australia. https://apps.aims.gov.au/metadata/view/31caee9f-a376-410a-b53b-419d5a37c694, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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adc@aims.gov.au

Brief description

Starch gel electrophoresis was used to examine genetic variation in five widely separated populations of the mangrove Rhizophora stylosa Griff. Shoots containing leaves and well developed apical buds were collected between March and August 1987 from Darwin, the Daintree River, 2 sites at Missionary Bay (Hinchinbrook Island), Chunda Bay (Cape Ferguson) and Lardelli Creek (Ayr). No two trees at each sampling site were separated by more than 1km, except for the Daintree River site, which was sampled over 2km.A suitable extraction buffer was developed by modifying a buffer developed by Dr Gavin Moran (CSIRO) for eucalypts and acacias.Twenty eight enzymes were investigated for the Rhizophora stylosa samples (ACP, ACON, AK, ADH, ALD, AAT, EST(FL), FH, BetaGAL, G6PD, GPI, GDH, GSR, GAPDH, HK, IDH, LAP, LGGP, LTP, LPP, MDH, ME, MDR, PER, PGM, 6PGD, SKDH, TPI). Fourteen enzymes were resolved adequately for these samples (ACON, AAT, GPI, GDH, GSR, HK, IDH, LAP, LTP, MDH, ME, PER, 6PGD, TPI).Other Rhizophoraceae successfully tested for enzyme activity in leaf and apical bud tissue, using the same methods were Rhizophora apiculata Blume, R. lamarckii Montr., R. mucronata Lamarck, Bruguiera gymnorhiza Lamarck, Ceriops decandra (Griff.) Ding Hou, C. tagal (Perr.) C.B. Rob. var. tagal and C. tagal var. australis C.T. White. Other mangrove species producing clear repeatable results included Lumnitzera racemosa Willdenow, Xylocarpus australasicus Ridley, Sonneratia alba J.E. Smith, Acrostichum speciosum Willdenow, Avicennia marina (Forssk.) Vierh. and A. officinalis Linnaeus.While leaves and buds were chosen initially, due to their year round availability, whole anthers and the plumule of the progagule of Rhizophora stylosa were also successfully tested for enzyme activity.
This research was undertaken to:1. develop an effective extraction buffer to counteract the effect of complex secondary metabolites in mangroves, which denature proteins during enzyme extraction.2. develop techniques for the electrophoresis of mangrove tissue and to use these to investigate genetic variability in Rhizophora stylosa and other Rhizophoraceae.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Statement: The techniques used for enzyme extraction and electrophoresis are described in the following paper:Goodall JA and Stoddart JA (1989) Techniques for the electrophoresis of mangrove tissue. Aquatic Botany 35: 197-207.

Notes

Credit
Goodall, John A, Mr (Principal Investigator)
Credit
Ballment, Elizabeth (Beth), Ms (Custodian)

Modified: 03 2024

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130.83333,-12.41667

130.833333,-12.416667

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145.483333,-16.3

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147.583333,-19.583333

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text: westlimit=147.583333; southlimit=-19.583333; eastlimit=147.583333; northlimit=-19.583333

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Other Information
Techniques for the electrophoresis of mangrove tissue: Goodall JA and Stoddart JA (1989) Techniques for the electrophoresis of mangrove tissue. Aquatic Botany 35: 197-207.

local : articleId=3461

Identifiers
  • global : 31caee9f-a376-410a-b53b-419d5a37c694
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