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Population genetics of the mangrove Rhizophora stylosa in northern Australia

data.gov.au
Australian Institute of Marine Science (Owned by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.gov.au/dataset/5b7d8199-e32b-4d40-9952-d6c380cca41d&rft.title=Population genetics of the mangrove Rhizophora stylosa in northern Australia&rft.identifier=population-genetics-of-the-mangrove-rhizophora-stylosa-in-northern-australia&rft.publisher=data.gov.au&rft.description=Point of truth URL of this metadata record - http://gcmd.nasa.gov/Resources/valids/archives/keyword_list.html - Starch gel electrophoresis was used to examine genetic variation in five widely separated populations of the mangrove Rhizophora stylosa Griff. Shoots containing leaves and well developed apical buds were collected between March and August 1987 from Darwin, the Daintree River, 2 sites at Missionary Bay (Hinchinbrook Island), Chunda Bay (Cape Ferguson) and Lardelli Creek (Ayr). No two trees at each sampling site were separated by more than 1km, except for the Daintree River site, which was sampled over 2km.\n\nA suitable extraction buffer was developed by modifying a buffer developed by Dr Gavin Moran (CSIRO) for eucalypts and acacias.\n\nTwenty eight enzymes were investigated for the Rhizophora stylosa samples (ACP, ACON, AK, ADH, ALD, AAT, EST(FL), FH, BetaGAL, G6PD, GPI, GDH, GSR, GAPDH, HK, IDH, LAP, LGGP, LTP, LPP, MDH, ME, MDR, PER, PGM, 6PGD, SKDH, TPI). Fourteen enzymes were resolved adequately for these samples (ACON, AAT, GPI, GDH, GSR, HK, IDH, LAP, LTP, MDH, ME, PER, 6PGD, TPI).\n\nOther Rhizophoraceae successfully tested for enzyme activity in leaf and apical bud tissue, using the same methods were Rhizophora apiculata Blume, R. lamarckii Montr., R. mucronata Lamarck, Bruguiera gymnorhiza Lamarck, Ceriops decandra (Griff.) Ding Hou, C. tagal (Perr.) C.B. Rob. var. tagal and C. tagal var. australis C.T. White. Other mangrove species producing clear repeatable results included Lumnitzera racemosa Willdenow, Xylocarpus australasicus Ridley, Sonneratia alba J.E. Smith, Acrostichum speciosum Willdenow, Avicennia marina (Forssk.) Vierh. and A. officinalis Linnaeus.\n\nWhile leaves and buds were chosen initially, due to their year round availability, whole anthers and the plumule of the progagule of Rhizophora stylosa were also successfully tested for enzyme activity.&rft.creator=Australian Institute of Marine Science&rft.date=2023&rft.coverage=130.833333,-12.416667&rft.coverage=130.833333,-12.416667&rft.coverage=true&rft_rights=Other&rft_subject=Acrostichum speciosum&rft_subject=Allozyme Electrophoresis&rft_subject=Angiosperms (flowering Plants)&rft_subject=Avicennia marina&rft_subject=Avicennia officinalis&rft_subject=Biological Classification&rft_subject=Bruguiera gymnorhiza&rft_subject=Ceriops decandra&rft_subject=Ceriops tagal var. australis&rft_subject=Ceriops tagal var. tagal&rft_subject=Coastal Processes&rft_subject=Genetic variation&rft_subject=Lumnitzera racemosa&rft_subject=Mangroves&rft_subject=Oceans&rft_subject=Plants&rft_subject=Rhizophora apiculata&rft_subject=Rhizophora lamarckii&rft_subject=Rhizophora mucronata&rft_subject=Rhizophora stylosa&rft_subject=Rhizophoraceae&rft_subject=Sonneratia alba&rft_subject=Xylocarpus australasicus&rft.type=dataset&rft.language=English Access the data
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Starch gel electrophoresis was used to examine genetic variation in five widely separated populations of the mangrove Rhizophora stylosa Griff. Shoots containing leaves and well developed apical buds were collected between March and August 1987 from Darwin, the Daintree River, 2 sites at Missionary Bay (Hinchinbrook Island), Chunda Bay (Cape Ferguson) and Lardelli Creek (Ayr). No two trees at each sampling site were separated by more than 1km, except for the Daintree River site, which was sampled over 2km.\n\nA suitable extraction buffer was developed by modifying a buffer developed by Dr Gavin Moran (CSIRO) for eucalypts and acacias.\n\nTwenty eight enzymes were investigated for the Rhizophora stylosa samples (ACP, ACON, AK, ADH, ALD, AAT, EST(FL), FH, BetaGAL, G6PD, GPI, GDH, GSR, GAPDH, HK, IDH, LAP, LGGP, LTP, LPP, MDH, ME, MDR, PER, PGM, 6PGD, SKDH, TPI). Fourteen enzymes were resolved adequately for these samples (ACON, AAT, GPI, GDH, GSR, HK, IDH, LAP, LTP, MDH, ME, PER, 6PGD, TPI).\n\nOther Rhizophoraceae successfully tested for enzyme activity in leaf and apical bud tissue, using the same methods were Rhizophora apiculata Blume, R. lamarckii Montr., R. mucronata Lamarck, Bruguiera gymnorhiza Lamarck, Ceriops decandra (Griff.) Ding Hou, C. tagal (Perr.) C.B. Rob. var. tagal and C. tagal var. australis C.T. White. Other mangrove species producing clear repeatable results included Lumnitzera racemosa Willdenow, Xylocarpus australasicus Ridley, Sonneratia alba J.E. Smith, Acrostichum speciosum Willdenow, Avicennia marina (Forssk.) Vierh. and A. officinalis Linnaeus.\n\nWhile leaves and buds were chosen initially, due to their year round availability, whole anthers and the plumule of the progagule of Rhizophora stylosa were also successfully tested for enzyme activity.

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130.83333,-12.41667

130.833333,-12.416667

130.83333,-12.41667

130.833333,-12.416667

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Subjects
Acrostichum speciosum | Allozyme Electrophoresis | Angiosperms (flowering Plants) | Avicennia marina | Avicennia officinalis | Biological Classification | Bruguiera gymnorhiza | Ceriops decandra | Ceriops tagal var. australis | Ceriops tagal var. tagal | Coastal Processes | Genetic variation | Lumnitzera racemosa | Mangroves | Oceans | Plants | Rhizophora apiculata | Rhizophora lamarckii | Rhizophora mucronata | Rhizophora stylosa | Rhizophoraceae | Sonneratia alba | Xylocarpus australasicus |

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