Data

P&O Nedlloyd Brisbane HPLC Pigment and Ocean Colour Data December 1999 - October 2000

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) Clementson, Lesley (Point of contact)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/bb6e58e6-d0c7-453f-95d9-8b3f4f362c06&rft.title=P&O Nedlloyd Brisbane HPLC Pigment and Ocean Colour Data December 1999 - October 2000&rft.identifier=Anzlic Identifier: ANZCW0306006378&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data collected on the P&O Nedlloyd Brisbane between December 1999 and October 2000 as part of the CSIRO Ocean Observing Experiment (COOE). This vessel circumnavigated Australia every 28 days with an additional stop in the Port of Singapore. Underway T, S and F were measured continuously throughout the period of the project. At times an observer was on board and collected samples from the outflow of the underway system for the determination of chlorophyll and carotenoid pigments, the absorption co-efficient for the dissolved component (aCDOM) of the water and salinity. In all 277 samples were collected during the 10 month period of the project. Retrieved chlorophyll a estimates from the SeaWifs ocean colour sensor were also collected and compared with the in situ data.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the P&O Nedlloyd Brisbane from the underway T,S and F system. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=112; southlimit=-44; eastlimit=154; northlimit=-9&rft.coverage=westlimit=112; southlimit=-44; eastlimit=154; northlimit=-9&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Countries | Australia&rft_subject=WfO Biogeochemistry&rft_subject=COOE - VOS Project (Chief Executive Special Project) 1999-2001&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data collected on the P&O Nedlloyd Brisbane between December 1999 and October 2000 as part of the CSIRO Ocean Observing Experiment (COOE). This vessel circumnavigated Australia every 28 days with an additional stop in the Port of Singapore. Underway T, S and F were measured continuously throughout the period of the project. At times an observer was on board and collected samples from the outflow of the underway system for the determination of chlorophyll and carotenoid pigments, the absorption co-efficient for the dissolved component (aCDOM) of the water and salinity. In all 277 samples were collected during the 10 month period of the project. Retrieved chlorophyll a estimates from the SeaWifs ocean colour sensor were also collected and compared with the in situ data.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the P&O Nedlloyd Brisbane from the underway T,S and F system. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.

Notes

Credit
COOE-VOS funding-Chief Executive Special Project 1999-2001
Credit
Lesley Clementson
Credit
Pru Bonham
Credit
Matthew Sherlock
Credit
Peter Jackson
Credit
John Parslow
Credit
Ken Suber
Credit
Jason Waring

Data time period: 1999-12-01 to 2000-10-09

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154,-9 154,-44 112,-44 112,-9 154,-9

133,-26.5

text: westlimit=112; southlimit=-44; eastlimit=154; northlimit=-9

Subjects
COOE - VOS Project (Chief Executive Special Project) 1999-2001 | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Countries | Australia | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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Donwload LC_IMOS_SRS_BioOptical_SOOP_voyage_AugSept2000_pigments.zip (.csv, .xls, .nc) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2016

Donwload LC_IMOS_SRS_BioOptical_SOOP_voyage_Dec1999_pigments.zip (.csv, .xls, .nc) (Data Link)

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Download LC_IMOS_SRS_BioOptical_SOOP_voyage_April2000_pigments.zip (.csv, .xls, .nc) (Data Link)

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Download LC_IMOS_SRS_BioOptical_SOOP_voyage_JanFeb2000_pigments.zip (.csv, .xls, .nc) (Data Link)

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Identifiers
  • Local : Anzlic Identifier: ANZCW0306006378
  • Local : Marlin Record Number: 6378
  • global : bb6e58e6-d0c7-453f-95d9-8b3f4f362c06