Dataset

Phytoplankton sampling in the coastal waters of south eastern Tasmania

University of Tasmania, Australia
Swadling, Kerrie, Dr
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://metadata.imas.utas.edu.au:/geonetwork/srv/en/metadata.show?uuid=3b002636-f1b2-441d-a135-f043df3c8c15&rft.title=Phytoplankton sampling in the coastal waters of south eastern Tasmania&rft.identifier=http://metadata.imas.utas.edu.au:/geonetwork/srv/en/metadata.show?uuid=3b002636-f1b2-441d-a135-f043df3c8c15&rft.publisher=Institute for Marine and Antarctic Studies, University of Tasmania&rft.description=Phytoplankton was counted and identified from five sites over the 5-year period. Annual cycles in abundance are available (as cells mL-1), along with detailed species identification. Cell measurements and approximate geometric shape were also recorded for the calculation of biovolume (μL cell-1). Diatoms and dinoflagellates dominated the samples in terms of biomass, however, small cells were also very abundant throughout each year. The data are restricted to an integrated sample from the top 12 m of the water column. Fluorescence profiles elsewhere in this dataset can provide an indication of phytoplankton presence lower in the water column.An integrated water column sample (12 m) using a weighted hose was collected for phytoplankton speciation and pigment analysis from February 2010. Prior to this, samples for phytoplankton speciation were collected from 1 m below the surface. Phytoplankton samples were concentrated by sequential settling over ~ 1 week, reducing the sample down to approximately 15 mL. The final volume was recorded and the sample thoroughly mixed before a 1 mL aliquot was taken and examined by phase contrast light microscopy (Leica DMLB2) in a Sedgewick-Rafter chamber. The entire chamber was scanned at low power (x50) to count large or rare species, and then re-examined at x200 until 400 squares had been inspected, or at least 200 cells of the dominant species had been counted. Thecate dinoflagellates were occasionally examined using fluorescence microscopy and Calcalfluor to highlight plate structure. The identification of species was confirmed at higher magnification (x400), and a minimum of 20 squares was examined at x400 to count small flagellates (&rft.creator=Swadling, Kerrie, Dr &rft.date=2018&rft.relation=https://data.imas.utas.edu.au/attachments/3b002636-f1b2-441d-a135-f043df3c8c15/2014-031-DLD.pdf&rft.coverage=northlimit=-43.0123609615; southlimit=-43.3548230459; westlimit=147.362548828; eastLimit=147.71685791&rft_rights=Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/&rft_rights=Please contact Kerrie Swadling (Principal Investigator) if you wish to use the data.&rft_rights=The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.&rft_subject=biota&rft_subject=PHYTOPLANKTON&rft_subject=EARTH SCIENCE&rft_subject=BIOSPHERE&rft_subject=AQUATIC ECOSYSTEMS&rft_subject=PLANKTON&rft_subject=AQUACULTURE&rft_subject=AGRICULTURE&rft_subject=AGRICULTURAL AQUATIC SCIENCES&rft_subject=WATER QUALITY/WATER CHEMISTRY&rft_subject=TERRESTRIAL HYDROSPHERE&rft_subject=OCEAN CURRENTS&rft_subject=OCEANS&rft_subject=OCEAN CIRCULATION&rft_subject=DINOFLAGELLATES&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=PROTISTS&rft_subject=FLAGELLATES&rft_subject=Storm Bay&rft_subject=Marine and Estuarine Ecology (incl. Marine Ichthyology)&rft_subject=Biological Oceanography&rft_subject=Temperate Reef&rft.type=dataset&rft.language=English Access the data

Licence & Rights:

Open Licence view details
CC-BY

Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/

Please contact Kerrie Swadling (Principal Investigator) if you wish to use the data.

The data described in this record are the intellectual property of the University of Tasmania through the Institute for Marine and Antarctic Studies.

Access:

Open

Full description

Phytoplankton was counted and identified from five sites over the 5-year period. Annual cycles in abundance are available (as cells mL-1), along with detailed species identification. Cell measurements and approximate geometric shape were also recorded for the calculation of biovolume (μL cell-1). Diatoms and dinoflagellates dominated the samples in terms of biomass, however, small cells were also very abundant throughout each year. The data are restricted to an integrated sample from the top 12 m of the water column. Fluorescence profiles elsewhere in this dataset can provide an indication of phytoplankton presence lower in the water column.

Lineage

An integrated water column sample (12 m) using a weighted hose was collected for phytoplankton speciation and pigment analysis from February 2010. Prior to this, samples for phytoplankton speciation were collected from 1 m below the surface. Phytoplankton samples were concentrated by sequential settling over ~ 1 week, reducing the sample down to approximately 15 mL. The final volume was recorded and the sample thoroughly mixed before a 1 mL aliquot was taken and examined by phase contrast light microscopy (Leica DMLB2) in a Sedgewick-Rafter chamber. The entire chamber was scanned at low power (x50) to count large or rare species, and then re-examined at x200 until 400 squares had been inspected, or at least 200 cells of the dominant species had been counted. Thecate dinoflagellates were occasionally examined using fluorescence microscopy and Calcalfluor to highlight plate structure. The identification of species was confirmed at higher magnification (x400), and a minimum of 20 squares was examined at x400 to count small flagellates (<5 μm), which were grouped into broad taxonomic categories based on shape and flagellae.

Created: 2018-06-25

Data time period: 2009-11-01 to 2015-04-30

This dataset is part of a larger collection

Click to explore relationships graph

147.71685791,-43.0123609615 147.71685791,-43.3548230459 147.362548828,-43.3548230459 147.362548828,-43.0123609615 147.71685791,-43.0123609615

147.539703369,-43.1835920037

Other Information
DATA ACCESS - Phytoplankton sampling [direct download]

uri : https://geoserver.imas.utas.edu.au/geoserver/wfs

URI : https://geoserver.imas.utas.edu.au/geoserver/wfs?version=1.0.0&request=GetFeature&typeName=imas:ECP_GEOSERVER_PHYTOPLANKTON&outputFormat=csv

MAP - Phytoplankton sampling locations (imas:ECP_GEOSERVER_PHYTOPLANKTON)

uri : https://geoserver.imas.utas.edu.au/geoserver/wms

This OGC WFS service returns the data in subsettable CSV format. (imas:ECP_GEOSERVER_PHYTOPLANKTON)

uri : https://geoserver.imas.utas.edu.au/geoserver/wfs

View and download this data through the interactive IMAS Data Portal.

uri : https://data.imas.utas.edu.au/portal/search

URI : https://data.imas.utas.edu.au/portal/search?uuid=3b002636-f1b2-441d-a135-f043df3c8c15