Data

Phytoplankton Ecology in the Upper Swan River Estuary, Western Australia - Seasonal nitrogen uptake rates and preferential uptake of inorganic and organic nitrogen and comparison of C:N ratios

Australian Ocean Data Network
Rosser, Jane
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://catalogue.aodn.org.au:443/geonetwork/srv/api/records/88db5e60-545a-11dc-8619-00188b4c0af8&rft.title=Phytoplankton Ecology in the Upper Swan River Estuary, Western Australia - Seasonal nitrogen uptake rates and preferential uptake of inorganic and organic nitrogen and comparison of C:N ratios&rft.identifier=https://catalogue.aodn.org.au:443/geonetwork/srv/api/records/88db5e60-545a-11dc-8619-00188b4c0af8&rft.description=The major aim of the study was to determine monthly and seasonal nitrogen uptake by phytoplankton communities of the three nitrogen species NO3-, NH4+ and urea from 1998 to 1999 at the Ron Courtney Island site, representing new inorganic-based production, regenerated inorganic-based production and organic-based production respectively.Maintenance and Update Frequency: notPlannedStatement: During the 1998-1999 monthly sampling regime, water was collected by positive displacement pump ffrom surface (0.25 m) and bottom (4.5 m) depths at the Ron Courtney Island site (see thumbnail) at noon (between 1200 h and 1300 h) and at night 1 - 2 hours before sunrise (between 0300 h and 600 h depending on season). All samples were gently pre-screened through a 300um Nitex screen, kept cool and transported under low light conditions to the laboratory within one hour of collection. Chlorophyll measurements were made from triplicate surface (0.25 m), mid-water (2.5 m) and bottom (4.5 m) water samples taken at the sample site. Chlorophyll determinations were made following standard methods (Strickland and Parsons, 1972). After sonication and overnight acetone extraction, sample absorbance was read on a Varian spectrophotometer. Calculations of total chlorophyll a (Chla) were made according to the equations of Jeffrey and Humphrey (1975, cited in Parsons et al., 1984). Samples for species composition analysis were collected by pooling the remaining water from the three 6-litre water samples, gently mixing and then sub-sampling. Species identification and enumeration were made from Lugols-preserved samples as previously described (see Chapter 3 of thesis). Nutrient and chlorophyll samples were filtered under low (< 250 mm Hg) pressure through GF/C filters. Filters and filtrate were stored frozen for later analysis. Ambient nutrient profiles for NO3- and NH4+ were obtained from the routine monitoring program by the Water and Rivers Commission or determined from samples sent to an analytical laboratory. Urea determinations using the diacetyl monoxime method (Price and Harrison, 1987), with a detection limit of 0.05 uM, were made on samples filtered under low (< 250 mm Hg) pressure.Statement: - Nitrogen uptake measurements - Nitrogen uptake rates were determined by the 15N dilution method of Dugdale and Goering (1967) as described in Chapter 4 of the thesis. See section 5.5.2 for more information.&rft.creator=Rosser, Jane &rft.date=2007&rft.coverage=westlimit=115.93; southlimit=-31.94; eastlimit=115.96; northlimit=-31.91&rft.coverage=westlimit=115.93; southlimit=-31.94; eastlimit=115.96; northlimit=-31.91&rft.coverage=uplimit=4.5; downlimit=0&rft.coverage=uplimit=4.5; downlimit=0&rft_rights=Creative Commons Attribution 2.5 Australia License http://creativecommons.org/licenses/by/2.5/au/&rft_rights=The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).&rft_subject=oceans&rft_subject=ESTUARIES&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=COASTAL PROCESSES&rft_subject=PHYTOPLANKTON&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=PROTISTS&rft_subject=PLANKTON&rft_subject=NITROGEN COMPOUNDS&rft_subject=TERRESTRIAL HYDROSPHERE&rft_subject=WATER QUALITY/WATER CHEMISTRY&rft_subject=NUTRIENT CYCLING&rft_subject=BIOSPHERE&rft_subject=ECOLOGICAL DYNAMICS&rft_subject=ECOSYSTEM FUNCTIONS&rft_subject=CHLOROPHYLL&rft.type=dataset&rft.language=English Access the data
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The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).

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The major aim of the study was to determine monthly and seasonal nitrogen uptake by phytoplankton communities of the three nitrogen species NO3-, NH4+ and urea from 1998 to 1999 at the Ron Courtney Island site, representing "new" inorganic-based production, regenerated inorganic-based production and organic-based production respectively.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: During the 1998-1999 monthly sampling regime, water was collected by positive displacement pump ffrom surface (0.25 m) and bottom (4.5 m) depths at the Ron Courtney Island site (see thumbnail) at noon (between 1200 h and 1300 h) and at night 1 - 2 hours before sunrise (between 0300 h and 600 h depending on season). All samples were gently pre-screened through a 300um Nitex screen, kept cool and transported under low light conditions to the laboratory within one hour of collection. Chlorophyll measurements were made from triplicate surface (0.25 m), mid-water (2.5 m) and bottom (4.5 m) water samples taken at the sample site. Chlorophyll determinations were made following standard methods (Strickland and Parsons, 1972). After sonication and overnight acetone extraction, sample absorbance was read on a Varian spectrophotometer. Calculations of total chlorophyll a (Chla) were made according to the equations of Jeffrey and Humphrey (1975, cited in Parsons et al., 1984). Samples for species composition analysis were collected by pooling the remaining water from the three 6-litre water samples, gently mixing and then sub-sampling. Species identification and enumeration were made from Lugols-preserved samples as previously described (see Chapter 3 of thesis). Nutrient and chlorophyll samples were filtered under low (< 250 mm Hg) pressure through GF/C filters. Filters and filtrate were stored frozen for later analysis. Ambient nutrient profiles for NO3- and NH4+ were obtained from the routine monitoring program by the Water and Rivers Commission or determined from samples sent to an analytical laboratory. Urea determinations using the diacetyl monoxime method (Price and Harrison, 1987), with a detection limit of 0.05 uM, were made on samples filtered under low (< 250 mm Hg) pressure.
Statement: - Nitrogen uptake measurements - Nitrogen uptake rates were determined by the 15N dilution method of Dugdale and Goering (1967) as described in Chapter 4 of the thesis. See section 5.5.2 for more information.

Notes

Purpose
To provide an insight into the mechanisms of bloom development and maintenance, as well as providing an insight into determining the most appropriate remediation strategies.

Created: 27 08 2007

Data time period: 1998-02 to 1999-01

This dataset is part of a larger collection

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115.96,-31.91 115.96,-31.94 115.93,-31.94 115.93,-31.91 115.96,-31.91

115.945,-31.925

text: westlimit=115.93; southlimit=-31.94; eastlimit=115.96; northlimit=-31.91

text: uplimit=4.5; downlimit=0

Subjects
BIOLOGICAL CLASSIFICATION | BIOSPHERE | Chlorophyll | COASTAL PROCESSES | EARTH SCIENCE | ECOLOGICAL DYNAMICS | ECOSYSTEM FUNCTIONS | Estuaries | Nitrogen Compounds | Nutrient Cycling | OCEANS | Phytoplankton | PLANKTON | PROTISTS | TERRESTRIAL HYDROSPHERE | WATER QUALITY/WATER CHEMISTRY | oceans |

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Other Information
(PhD Thesis)

url : http://adt.curtin.edu.au/theses/available/adt-WCU20051213.114126/

global : 8cff8db0-511a-11dc-a105-00188b4c0af8

Identifiers
  • global : 88db5e60-545a-11dc-8619-00188b4c0af8