Full description
Maximum nitrogen uptake rates (Vmax) and preferential nitrogen sources were determined for the different major bloom types between November 1995 and May 1996 in the upper Swan River Estuary. These uptake rates and preferences were related to the pattern of succession for phytoplankton blooms and diurnal changes in ambient nutrient concentrations for NH4+ in the summer bloom.
Lineage
Maintenance and Update Frequency: notPlanned
Statement: Chlorophyte-dominated (spring, November 1995 and 197), and mixed Dinophyte-dominated (summer, January 1996 and autumn, May 1996) bloom events were studied at Ron Courtney Island (RCI), an area representative of the deeper (~6 m) sites of the upper Swan River Estuary. Chlorophyll measurements were made from triplicate surface (0.25 m), mid-water (2.5 m) and bottom (4.5 m) water samples taken at the sample site, and from profiles (1m increments) made at RCI and several sites above and below the RCI site. This sampling was to verify that trends in chlorophyll distribution patterns seen at the RCI experimental site were representative of trends occuring over a larger area of the river, and not attributable to local patchiness or advection. Samples for species composition analysis were collected by pooling the remaining water from the three 6-1 water samples (collected for nitrogen uptake determinations, see below), gently mixing and then sub-sampling. Species identification and enumeration were made from Lugols-preserved samples as described in Chapter 3 of thesis.
Ambient nutrient profiles for NO3- and NH4+ were obtained from the routine monitoring program by the Water and Rivers Commission or determined from samples sent to an analytical laboratory. Nutrient and chlorophyll samples were filtered through GF/C filters and the filters stored frozen for later analysis. Nitrate was measured by the cadmium reduction method and ammounium was measured by the phenate method (Clesceri et al., 1989). Chlorophyll determinations were made following standard methods (Strickland and Parsons, 1972).
***References***
Clesceri, L. S., A. E. Greenberg and R. R. Trussell, (eds). 1989. Standard methods for the examination of water and waste water. 17th edition. Published jointly by American Health Association, American Water Works Association, Water Pollution Control Federation. Washington D.C.
Strickland, J. D. H., and T. R. Parsons. 1972. A practical handbook of seawater analysis. 2nd Edn. Bulletin of the Fisheries Research Board of Canada No. 167. 310pp.
Statement: - Nitrogen uptake -
Nitrogen uptake rates were determined by the 15N dilution method of Dugdale and Goering (1967) from water samples collected at the RCI site. For seasonal bloom studies three depths (surface, 0.25 m; midwater, 2.5 m abd bottom, 4.5 m) were sampled. The spring (November) and summer (January) blooms were sampled at three times (approx. 0900 h-am, 1400 h-pm and 2400 h-night) over a diurnal period. The autumn bloom (May) was sampled twice (1200 h, noon and 0300 - 0400 h, night). Water samples were collected as described in Chapter 4 methods and processed as described in Chapter 5 for 15N uptake rate determinations. All specific bloom studies were incubated in situ at the depth of collection to maintain ambient light and temperature conditions. Bottles were suspended in purpose built racks in a staggered manner to ensure no shading occurred from those at shallower depths.
***References***
Dugdale, R. C. and J. J. Goering. 1967. Uptake of new and regenerated forms of nitrogen in primary productivity. Limnology and Oceanography 12: 196-206
Notes
Purpose
To provide an insight into the mechanisms of local bloom development and maintenance.