Data

PCR genetic screening for presence of sea urchin DNA in rock lobster faecal samples

Australian Ocean Data Network
Johnson, Craig, Prof. ; Ling, Scott, Dr ; Redd, Kevin
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/665c701d-0737-4093-8ba0-93765fc12c94&rft.title=PCR genetic screening for presence of sea urchin DNA in rock lobster faecal samples&rft.identifier=https://metadata.imas.utas.edu.au/geonetwork/srv/eng/catalog.search#/metadata/665c701d-0737-4093-8ba0-93765fc12c94&rft.description=Data is PCR amplification results of southern rock lobster (Jasus edwardsii) faecal material tested for sea urchin DNA (using unique primers for Centrostephanus rodgersii and Heliocidaris erythrogramma) in an attempt to determine in situ rates of consumption of sea urchins by lobsters. An efficient and non-lethal method was used to source and screen lobster faecal samples for the presence of DNA from ecologically important sea urchins. Lobster faecal samples were collected from trap caught specimens sourced in winter & summer seasons over 2 years (2009-2011) within two no-take research reserves; declared specifically for the purpose of rebuilding large predatory-capable lobsters to assess the potential for predator-driven remediation of kelp beds on rocky reefs extensively overgrazed by sea urchins (North Eastern Tasmania) and reefs showing initial signs of overgrazing (South Eastern Tasmania). Data for molecular assays showed high variability in the proportion of lobsters testing positive to sea urchins, with significant variability detected across different years and seasons but this was found to vary depending on different lobster size-classes. Sea urchin DNA was also amplifiable from sediments and urchin faeces collected from the reef surface where urchins occurred in high abundance. Furthermore, positive sea urchin DNA assays were obtainable from lobster faeces after lobsteres were fed sediment and urchin faecal material. Rates of predation obtained with genetics tests can also be compared to independent rates of urchin losses given known lobster abundances within research reserves (and at control sites). Data of changes in urchin abundances and lobster abundances are therefore also lodged as part of this record.Maintenance and Update Frequency: notPlannedStatement: PCR detection of urchin DNA in lobster faecal material. Refer to Data file help document” for general methods information, and for a full description of methods see the Draft Final Report of the Fisheries Research & Development Corporation research project #FRDC2007_045.Statement: GPS used datum WGS84.&rft.creator=Johnson, Craig, Prof. &rft.creator=Ling, Scott, Dr &rft.creator=Redd, Kevin &rft.date=2011&rft.coverage=westlimit=148.25000000000003; southlimit=-41.35; eastlimit=148.45; northlimit=-41.15&rft.coverage=westlimit=148.25000000000003; southlimit=-41.35; eastlimit=148.45; northlimit=-41.15&rft.coverage=westlimit=147.85; southlimit=-42.95000000000001; eastlimit=148.05; northlimit=-42.75&rft.coverage=westlimit=147.85; southlimit=-42.95000000000001; eastlimit=148.05; northlimit=-42.75&rft.coverage=uplimit=40; downlimit=3&rft.coverage=uplimit=40; downlimit=3&rft_rights=Creative Commons Attribution-Share Alike 2.5 Australia License http://creativecommons.org/licenses/by-sa/2.5/au/&rft_rights=The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).&rft_rights=See point of contact for access to the data.&rft_rights=Use of this data is currently embargoed until publication in the peer-review literature.&rft_rights=The data described in this record are the intellectual property of University of Tasmania through the Institute for Marine and Antarctic Studies&rft_subject=biota&rft_subject=Centrostephanus rodgersii&rft_subject=Jasus edwardsii&rft_subject=Haliotis rubra&rft_subject=Ecklonia radiata&rft_subject=Heliocidaris erythrogramma&rft_subject=Genetics&rft_subject=Urchin Barrens&rft_subject=Kelp Beds&rft_subject=DNA Detection&rft_subject=ANIMALS/INVERTEBRATES&rft_subject=EARTH SCIENCE&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=REEF HABITAT&rft_subject=BIOSPHERE&rft_subject=AQUATIC ECOSYSTEMS&rft_subject=TROPHIC DYNAMICS&rft_subject=ECOLOGICAL DYNAMICS&rft_subject=ECOSYSTEM FUNCTIONS&rft_subject=COMMUNITY STRUCTURE&rft_subject=COMMUNITY DYNAMICS&rft_subject=MARINE HABITAT&rft_subject=ECHINODERMS&rft_subject=CRUSTACEANS&rft_subject=ARTHROPODS&rft_subject=SEA URCHINS&rft_subject=Temperate Reef&rft_subject=Fish Physiology and Genetics&rft_subject=AGRICULTURAL AND VETERINARY SCIENCES&rft_subject=FISHERIES SCIENCES&rft_subject=Marine and Estuarine Ecology (incl. Marine Ichthyology)&rft_subject=BIOLOGICAL SCIENCES&rft_subject=ECOLOGY&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution-Share Alike 2.5 Australia License
http://creativecommons.org/licenses/by-sa/2.5/au/

The citation in a list of references is: citation author name/s (year metadata published), metadata title. Citation author organisation/s. File identifier and Data accessed at (add http link).

See point of contact for access to the data.

Use of this data is currently embargoed until publication in the peer-review literature.

The data described in this record are the intellectual property of University of Tasmania through the Institute for Marine and Antarctic Studies

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Brief description

Data is PCR amplification results of southern rock lobster (Jasus edwardsii) faecal material tested for sea urchin DNA (using unique primers for Centrostephanus rodgersii and Heliocidaris erythrogramma) in an attempt to determine in situ rates of consumption of sea urchins by lobsters. An efficient and non-lethal method was used to source and screen lobster faecal samples for the presence of DNA from ecologically important sea urchins. Lobster faecal samples were collected from trap caught specimens sourced in winter & summer seasons over 2 years (2009-2011) within two no-take research reserves; declared specifically for the purpose of rebuilding large predatory-capable lobsters to assess the potential for predator-driven remediation of kelp beds on rocky reefs extensively overgrazed by sea urchins (North Eastern Tasmania) and reefs showing initial signs of overgrazing (South Eastern Tasmania). Data for molecular assays showed high variability in the proportion of lobsters testing positive to sea urchins, with significant variability detected across different years and seasons but this was found to vary depending on different lobster size-classes. Sea urchin DNA was also amplifiable from sediments and urchin faeces collected from the reef surface where urchins occurred in high abundance. Furthermore, positive sea urchin DNA assays were obtainable from lobster faeces after lobsteres were fed sediment and urchin faecal material. Rates of predation obtained with genetics tests can also be compared to independent rates of urchin losses given known lobster abundances within research reserves (and at control sites). Data of changes in urchin abundances and lobster abundances are therefore also lodged as part of this record.

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Maintenance and Update Frequency: notPlanned
Statement: PCR detection of urchin DNA in lobster faecal material. Refer to "Data file help document” for general methods information, and for a full description of methods see the Draft Final Report of the Fisheries Research & Development Corporation research project #FRDC2007_045.
Statement: GPS used datum WGS84.

Notes

Credit
Funding: FRDC project 2007/045
Purpose
Assessment of Management Options to Minimise Formation of 'Barrens' Habitat by the Long-Spined Sea Urchin (Centrostephanus rodgersii) in Tasmania

Created: 16 09 2011

Data time period: 04 2008 to 28 02 2011

This dataset is part of a larger collection

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148.45,-41.15 148.45,-41.35 148.25,-41.35 148.25,-41.15 148.45,-41.15

148.35,-41.25

148.05,-42.75 148.05,-42.95 147.85,-42.95 147.85,-42.75 148.05,-42.75

147.95,-42.85

text: westlimit=148.25000000000003; southlimit=-41.35; eastlimit=148.45; northlimit=-41.15

text: westlimit=147.85; southlimit=-42.95000000000001; eastlimit=148.05; northlimit=-42.75

text: uplimit=40; downlimit=3

Subjects
Agricultural and Veterinary Sciences | ANIMALS/INVERTEBRATES | AQUATIC ECOSYSTEMS | ARTHROPODS | BIOLOGICAL CLASSIFICATION | Biological Sciences | BIOSPHERE | COMMUNITY DYNAMICS | COMMUNITY STRUCTURE | CRUSTACEANS | Centrostephanus rodgersii | DNA Detection | EARTH SCIENCE | ECHINODERMS | ECOLOGICAL DYNAMICS | Ecology | ECOSYSTEM FUNCTIONS | Ecklonia radiata | Fisheries Sciences | Fish Physiology and Genetics | Genetics | Haliotis rubra | Heliocidaris erythrogramma | Jasus edwardsii | Kelp Beds | MARINE HABITAT | Marine and Estuarine Ecology (Incl. Marine Ichthyology) | REEF HABITAT | SEA URCHINS | TROPHIC DYNAMICS | Temperate Reef | Urchin Barrens | biota |

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Other Information
(DATA - Genetic tests on lobster faeces [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/1.FRDC2007_045_genetic_tests_on_lobster_faeces.xlsx

(DATA - Genetic tests of sea urchin DNA in environmental samples [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/2.FRDC2007_045_genetic_tests_on_environmental_samples.xlsx

(DATA - Genetic tests of sea urchin faecal samples [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/3.FRDC2007_045_genetic_tests_on_urchin_faeces.xlsx

(DATA - Genetic tests for sea urchin DNA in lobster faeces fed environmental and sea urchin faecal samples [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/4.FRDC2007_045_genetic_tests_on_lobster_faeces_fed_env_samples.xlsx

(DATA - Sea urchin density change within Research Reserves [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/5.FRDC2007_045_Urchin_delta_within_Research_Reserves.xlsx

(DATA - Sea urchin density change within Research Reserves relative to control sites [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/6.FRDC2007_045_Urchin_delta_relative_to_controls.xlsx

(README - data file help document [direct download])

uri : https://data.imas.utas.edu.au/attachments/665c701d-0737-4093-8ba0-93765fc12c94/FRDC2007_05_Genetics_data_file_descriptions.docx

Identifiers
  • global : 665c701d-0737-4093-8ba0-93765fc12c94