Data

PacBio HiFi DNA sequencing reads of the leaf rust fungus Puccinia triticina

Commonwealth Scientific and Industrial Research Organisation
Dodds, Peter ; Figueroa, Melania
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25919/xbqb-px51&rft.title=PacBio HiFi DNA sequencing reads of the leaf rust fungus Puccinia triticina&rft.identifier=https://doi.org/10.25919/xbqb-px51&rft.publisher=Commonwealth Scientific and Industrial Research Organisation&rft.description=PacBio HiFi DNA sequencing reads of the leaf rust fungus Puccinia triticina for fully-phased, chromosome-scale genome assembly. High molecular DNA from urediniospores was extracted and PacBio HiFi reads were sequenced.\nLineage: Rust infected samples from the leaf rust susceptible wheat cultivar Morocco were collected from the CSIRO field site in Canberra during November 2018. A Puccinia triticina (Pt) culture was purified through single pustule isolation and pathotyped using the standard wheat differential sets carrying unique resistance genes for leaf rust as described in the Cereal Rust Report, PBI. Based on the resistance response, the isolate belongs to 76-3,5,7,9,10,12,13 pathotype and was named Pt76.&rft.creator=Dodds, Peter &rft.creator=Figueroa, Melania &rft.date=2021&rft.edition=v2&rft_rights=CSIRO Data Licence https://research.csiro.au/dap/licences/csiro-data-licence/&rft_rights=Data is accessible online and may be reused in accordance with licence conditions&rft_rights=All Rights (including copyright) CSIRO 2021.&rft_subject=HiFi&rft_subject=PacBio&rft_subject=long reads&rft_subject=genome&rft_subject=Animal protection (incl. pests and pathogens)&rft_subject=Animal production&rft_subject=AGRICULTURAL, VETERINARY AND FOOD SCIENCES&rft_subject=Genomics&rft_subject=Genetics&rft_subject=BIOLOGICAL SCIENCES&rft.type=dataset&rft.language=English Access the data

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Brief description

PacBio HiFi DNA sequencing reads of the leaf rust fungus Puccinia triticina for fully-phased, chromosome-scale genome assembly. High molecular DNA from urediniospores was extracted and PacBio HiFi reads were sequenced.
Lineage: Rust infected samples from the leaf rust susceptible wheat cultivar Morocco were collected from the CSIRO field site in Canberra during November 2018. A Puccinia triticina (Pt) culture was purified through single pustule isolation and pathotyped using the standard wheat differential sets carrying unique resistance genes for leaf rust as described in the Cereal Rust Report, PBI. Based on the resistance response, the isolate belongs to 76-3,5,7,9,10,12,13 pathotype and was named Pt76.

Available: 2021-04-27

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