Data

Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates

Australian Ocean Data Network
McInnes, J.C., Deagle, B.E., Alderman, R., Jarman, S.N., Lea, M-A. and Raymond, B. ; MCINNES, JULIE C. ; DEAGLE, BRUCE E. ; ALDERMAN, RACHAEL ; JARMAN, SIMON N. ; LEA, MARY-ANNE ; RAYMOND, BEN
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2016&rft.title=Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates&rft.identifier=http://data.aad.gov.au/metadata/records/AAS_4014_McInnes_et_al_2016&rft.publisher=Australian Antarctic Data Centre&rft.description=These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition) Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution.Progress Code: completedStatement: See the referenced paper for more information.&rft.creator=McInnes, J.C., Deagle, B.E., Alderman, R., Jarman, S.N., Lea, M-A. and Raymond, B. &rft.creator=MCINNES, JULIE C. &rft.creator=DEAGLE, BRUCE E. &rft.creator=ALDERMAN, RACHAEL &rft.creator=JARMAN, SIMON N. &rft.creator=LEA, MARY-ANNE &rft.creator=RAYMOND, BEN &rft.date=2016&rft.coverage=westlimit=144.65; southlimit=-40.383333; eastlimit=144.65; northlimit=-40.383333&rft.coverage=westlimit=144.65; southlimit=-40.383333; eastlimit=144.65; northlimit=-40.383333&rft_rights=This metadata record is publicly available.&rft_rights=These data are publicly available for download from the provided URL.&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=Please contact the chief investigator of this work if you wish to use this data set for future analysis. This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_AAS_4014_McInnes_et_al_2016 when using these data. http://creativecommons.org/licenses/by/4.0/).&rft_rights=Portable Network Graphic&rft_rights=https://i.creativecommons.org/l/by/3.0/88x31.png&rft_rights=Creative Commons by Attribution logo&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights=Legal code for Creative Commons by Attribution 4.0 International license&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES&rft_subject=DNA METABARCODING&rft_subject=MISEQ GENOME SEQUENCING&rft_subject=SHY ALBATROSS&rft_subject=DIET&rft_subject=THALASSARCHE CAUTA&rft_subject=ALBATROSS ISLAND&rft_subject=AMD/AU&rft_subject=AMD&rft_subject=CEOS&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania&rft.type=dataset&rft.language=English Access the data
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Please contact the chief investigator of this work if you wish to use this data set for future analysis.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4014_AAS_4014_McInnes_et_al_2016 when using these data.
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This metadata record is publicly available.

These data are publicly available for download from the provided URL.

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Brief description

These spreadsheets provide the sequences counts for the DNA groups found in the scats of Shy Albatross at Albatross Island, Tasmania, Australia. Samples were collected during three stages of the breeding season: Incubation (Late September 2014), Brood (December 2014) and Chick-rearing (March 2014 and 2015). Scat samples were collected from breeding birds, chicks and non-breeders. 'Breeders' were identified as individuals incubating an egg or attending a chick, whereas 'non-breeders' were usually pairs that had reoccupied the colony and were building new practice nests with no chick present. Non-breeders in the colony include immature birds that have not yet bred and mature birds of breeding age that did not breed in a particular season (e.g. no partner or insufficient body condition) Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Deagle, B.E. Lea, M-A., Raymond, B. and Jarman, S.N. 2016. Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates. Methods in Ecology and Evolution.

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Progress Code: completed
Statement: See the referenced paper for more information.

Notes

Purpose
Samples were collected to determine the affect that sample age, collection substrate, breeding stage and incubation duration had on the DNA amplification success and the proportion of food DNA. Using these data, optimised scat collection protocols were developed.

Data time period: 2014-03-28 to 2015-03-31

This dataset is part of a larger collection

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144.65,-40.38333

144.65,-40.383333

text: westlimit=144.65; southlimit=-40.383333; eastlimit=144.65; northlimit=-40.383333

Subjects
ALBATROSS ISLAND | AMD | AMD/AU | CEOS | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA > Tasmania | DIET | DNA METABARCODING | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > BIRDS > ALBATROSSES/PETRELS AND ALLIES | GEOGRAPHIC REGION > POLAR | MISEQ GENOME SEQUENCING | OCEAN > SOUTHERN OCEAN | SHY ALBATROSS | THALASSARCHE CAUTA | biota | oceans |

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  • global : AAS_4014_McInnes_et_al_2016
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