Data

National Environmental Research Program (NERP) cruise, Darwin, September 2013, HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) CSIRO/Oceans and Atmosphere (Associated with)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/1e5565e0-9969-5a98-e053-08114f8cfeac&rft.title=National Environmental Research Program (NERP) cruise, Darwin, September 2013, HPLC Pigment and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306014593&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data collected at a total of 30 stations on an unknown vessel, off the coast of Darwin. The samples were collected from September 09-15 2013 as part of the National Environmental Research Program. The data can be used for Ocean Colour sensor validation. Parameters measured include Total Suspended Particulate Matter (TSM), including the organic and inorganic fractions; the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory at CMAR, Hobart. Pigment analysis; approximately 2 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption; approximately 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a Cinta 404 UV/VIS dual beam spectrophotometer equipped with an integrating sphere&rft.creator=Anonymous&rft.date=2015&rft.coverage=westlimit=130.75; southlimit=-12.532; eastlimit=132.477; northlimit=-11.453&rft.coverage=westlimit=130.75; southlimit=-12.532; eastlimit=132.477; northlimit=-11.453&rft.coverage=uplimit=0; downlimit=0&rft.coverage=uplimit=0; downlimit=0&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Global / Oceans | World&rft_subject=Marine Features (Australia) | Australian EEZ&rft_subject=Regional Seas | Timor Sea&rft_subject=States, Territories (Australia) | Northern Territory&rft_subject=National Environmental Research Programme (NERP) - Northern Hub&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

Access:

Open

Full description

This record contains data collected at a total of 30 stations on an unknown vessel, off the coast of Darwin. The samples were collected from September 09-15 2013 as part of the National Environmental Research Program. The data can be used for Ocean Colour sensor validation. Parameters measured include Total Suspended Particulate Matter (TSM), including the organic and inorganic fractions; the absorption coefficients of the particulate and dissolved components of the water column and the phytoplankton pigment concentration and composition.

Lineage

Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory at CMAR, Hobart. Pigment analysis; approximately 2 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption; approximately 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a Cinta 404 UV/VIS dual beam spectrophotometer equipped with an integrating sphere

Notes

Credit
Thomas Schroeder (CSIRO O&A Dutton Park)
Credit
Lesley Clementson (CSIRO O&A Hobart)
Credit
Heidi Franklin (CSIRO O&A Dutton Park)

Data time period: 2013-09-09 to 2013-09-15

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related People

132.477,-11.453 132.477,-12.532 130.75,-12.532 130.75,-11.453 132.477,-11.453

131.6135,-11.9925

text: westlimit=130.75; southlimit=-12.532; eastlimit=132.477; northlimit=-11.453

text: uplimit=0; downlimit=0

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | Global / Oceans | World | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Australian EEZ | National Environmental Research Programme (NERP) - Northern Hub | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Regional Seas | Timor Sea | Spectrophotometers | States, Territories (Australia) | Northern Territory | Temperature of the water body | oceans | research vessel |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
(Data Link)

url : https://imos.org.au/

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

url : https://creativecommons.org/licenses/by/4.0/

Identifiers
  • Local : Anzlic Identifier: ANZCW0306014593
  • Local : Marlin Record Number: 14593
  • global : 1e5565e0-9969-5a98-e053-08114f8cfeac