Data

Moreton Bay, January 2003, HPLC Pigment and Ocean Colour Data

Integrated Marine Observing System
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with) Clementson, Lesley (Point of contact)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/ddbf5418-7cca-549f-e043-08114f8cabe3&rft.title=Moreton Bay, January 2003, HPLC Pigment and Ocean Colour Data&rft.identifier=Anzlic Identifier: ANZCW0306014229&rft.publisher=Integrated Marine Observing System&rft.description=This record contains data generated from samples collected in Moreton Bay between 28 and 31 Jan 2003. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. 8 stations were sampled. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and absorption coefficient, dissolved and particulate. CLW deployed instruments RAMSES, AC-9, Hydroscat and Hydrorad.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.&rft.creator=Anonymous&rft.date=2013&rft.coverage=westlimit=153.1; southlimit=-27.7; eastlimit=153.4; northlimit=-26.7&rft.coverage=westlimit=153.1; southlimit=-27.7; eastlimit=153.4; northlimit=-26.7&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=oceans&rft_subject=Earth Science | Oceans | Ocean Chemistry | Chlorophyll&rft_subject=Earth Science | Oceans | Ocean Chemistry | Pigments&rft_subject=Earth Science | Oceans | Ocean Optics | Ocean Color&rft_subject=Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Marine Features (Australia) | Moreton Bay, QLD&rft_subject=WfO Biogeochemistry&rft_subject=Remote Sensing 2000-2003&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=CTD&rft_subject=research vessel&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence, please see link. Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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This record contains data generated from samples collected in Moreton Bay between 28 and 31 Jan 2003. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital or non-algal (a/d) components of the water column. 8 stations were sampled. The data was used primarily to validate ocean colour sensors MERIS, MODIS and SeaWIFs and the SST sensor AATSR. Samples were collected and analysed for pigments, total suspended solids (TSS) and absorption coefficient, dissolved and particulate. CLW deployed instruments RAMSES, AC-9, Hydroscat and Hydrorad.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.

Notes

Credit
Ian Barton
Credit
Lesley Clementson (CMAR)
Credit
Kadija Oobelkheir(CLW)
Credit
Wojciech Klonowski
Credit
Chris Roelfsema

Data time period: 2003-01-28 to 2003-01-31

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153.4,-26.7 153.4,-27.7 153.1,-27.7 153.1,-26.7 153.4,-26.7

153.25,-27.2

text: westlimit=153.1; southlimit=-27.7; eastlimit=153.4; northlimit=-26.7

Subjects
CTD | CTDs (Conductivity-Temperature-Depth Profilers) | Earth Science | Oceans | Ocean Chemistry | Chlorophyll | Earth Science | Oceans | Ocean Chemistry | Pigments | Earth Science | Oceans | Ocean Optics | Ocean Color | Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll | Fluorometers | HPLC (High Performance Liquid Chromatography) | Marine Features (Australia) | Moreton Bay, QLD | Niskin Bottles | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Remote Sensing 2000-2003 | Spectrophotometers | Temperature of the water body | WfO Biogeochemistry | oceans | research vessel |

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url : https://aesop.csiro.au/

Data also available via IMOS and AODN portal (Data Link)

url : https://portal.aodn.org.au/

Download LC_IMOS_SRS_BioOptical_MB_Jan_2003_TSS.zip (.csv, .xls, .nc) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2933

Download LC_IMOS_SRS_BioOptical_MB_Jan_2003_absorption.zip (.csv, .xls, .nc) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2934

Download LC_IMOS_SRS_BioOptical_MB_Jan_2003_pigments.zip (.csv, .xls, .nc) (Data Link)

url : https://www.marine.csiro.au/data/trawler/download.cfm?file_id=2935

This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)

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Identifiers
  • Local : Anzlic Identifier: ANZCW0306014229
  • Local : Marlin Record Number: 14229
  • global : ddbf5418-7cca-549f-e043-08114f8cabe3