Data

Molecular characterisation of a haplosporidian parasite infecting rock oysters Saccostrea cuccullata in north Western Australia

Australian Ocean Data Network
Luke Edwards (Distributes) Murdoch Manager (Point of contact) School of Veterinary and Life Sciences (VLS), Murdoch University (Associated with) iVEC (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://catalogue.aodn.org.au:443/geonetwork/srv/api/records/516811d7-cd72-207a-e0440003ba8c79dd&rft.title=Molecular characterisation of a haplosporidian parasite infecting rock oysters Saccostrea cuccullata in north Western Australia&rft.identifier=516811d7-cd72-207a-e0440003ba8c79dd&rft.publisher=Australian Ocean Data Network&rft.description=A haplosporidian parasite was identified in rock oysters (Saccostrea cuccullata Born, 1778) from the Montebello Islands (latitude -20.4'S longitude 115.53'E) off the northern coast of Western Australia by histopathological examination, PCR amplification and DNA sequencing of a segment of the SSU region of the parasite's rRNA gene. An oligonucleotide probe was constructed from the parasite's SSU rRNA gene in order to confirm its presence by in situ hybridisation. The parasite was disseminated throughout the gonad follicles of the host and to a lesser extent in the gills. The only parasite life stages thus far observed in this study were a uninucleate naked cell assumed to be a precursor to multinucleate plasmodial stages and a binucleate plasmodial stage. Whilst no parasite spores were detected in affected rock oysters, a phylogenetic analysis of the SSU region of the parasite's rRNA gene indicates the parasite belongs to the genus Minchinia. A PCR and in situ hybridisation assay for the Minchinia sp. was used to identify haplosporidians described by Hine and Thorne [Hine, P.M.., Thorne, T., 2002. Haplosporidium sp. (Haplosporidia: Haplosporidiidae) associated with mortalities among rock oysters Saccostrea cuccullata in north Western Australia. Dis. Aquat. Organ. 51, 123-13], in archived rock oyster tissues from the same coastline.Maintenance and Update Frequency: unknownStatement: Original record compiled for the Western Australian Marine Science Institution (WAMSI), Project 3.8, 2008. Originally sourced from WA DEC Marine Policy and Planning Branch Pilbara and Lower West Kimberley Environmental Report Library. Date range generated from citation date, limited abstract information available.&rft.creator=Anonymous&rft.date=2017&rft.coverage=westlimit=115.48; southlimit=-20.52; eastlimit=115.62; northlimit=-20.36&rft.coverage=westlimit=115.48; southlimit=-20.52; eastlimit=115.62; northlimit=-20.36&rft_rights=No Restrictions&rft_subject=biota&rft_subject=Rock Oyster&rft_subject=Fauna&rft_subject=Genetics&rft_subject=Parasite&rft.type=dataset&rft.language=English Access the data
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A haplosporidian parasite was identified in rock oysters (Saccostrea cuccullata Born, 1778) from the Montebello Islands (latitude -20.4'S longitude 115.53'E) off the northern coast of Western Australia by histopathological examination, PCR amplification and DNA sequencing of a segment of the SSU region of the parasite's rRNA gene. An oligonucleotide probe was constructed from the parasite's SSU rRNA gene in order to confirm its presence by in situ hybridisation. The parasite was disseminated throughout the gonad follicles of the host and to a lesser extent in the gills. The only parasite life stages thus far observed in this study were a uninucleate naked cell assumed to be a precursor to multinucleate plasmodial stages and a binucleate plasmodial stage. Whilst no parasite spores were detected in affected rock oysters, a phylogenetic analysis of the SSU region of the parasite's rRNA gene indicates the parasite belongs to the genus Minchinia. A PCR and in situ hybridisation assay for the Minchinia sp. was used to identify haplosporidians described by Hine and Thorne [Hine, P.M.., Thorne, T., 2002. Haplosporidium sp. (Haplosporidia: Haplosporidiidae) associated with mortalities among rock oysters Saccostrea cuccullata in north Western Australia. Dis. Aquat. Organ. 51, 123-13], in archived rock oyster tissues from the same coastline.

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Maintenance and Update Frequency: unknown
Statement: Original record compiled for the Western Australian Marine Science Institution (WAMSI), Project 3.8, 2008. Originally sourced from WA DEC Marine Policy and Planning Branch Pilbara and Lower West Kimberley Environmental Report Library. Date range generated from citation date, limited abstract information available.

Notes

Credit
D. Bearham
Credit
Z. Spiers
Credit
S. Raidal
Credit
J.B. Jones
Credit
P.K. Nicholls
Credit
School of Veterinary Science, Murdoch University

Modified: 06 2008

Data time period: 2006 to 2006

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115.62,-20.36 115.62,-20.52 115.48,-20.52 115.48,-20.36 115.62,-20.36

115.55,-20.44

text: westlimit=115.48; southlimit=-20.52; eastlimit=115.62; northlimit=-20.36

Subjects
Fauna | Genetics | Parasite | Rock Oyster | biota |

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  • global : 516811d7-cd72-207a-e0440003ba8c79dd