Data

Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria

Australian Antarctic Division
Codd, R. ; CODD, RACHEL
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/ASAC_2547&rft.title=Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria&rft.identifier=http://data.aad.gov.au/metadata/ASAC_2547&rft.publisher=Australian Antarctic Data Centre&rft.description=Metadata record for data from ASAC Project 2547See the link below for public details on this project.Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this. This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]).The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.Progress Code: completedStatement: The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012.&rft.creator=Codd, R. &rft.creator=CODD, RACHEL &rft.date=2005&rft.coverage=westlimit=76; southlimit=-67; eastlimit=79; northlimit=-65&rft.coverage=westlimit=76; southlimit=-67; eastlimit=79; northlimit=-65&rft.coverage=westlimit=70; southlimit=-68; eastlimit=80; northlimit=-65&rft.coverage=westlimit=70; southlimit=-68; eastlimit=80; northlimit=-65&rft.coverage=uplimit=100; downlimit=0&rft.coverage=uplimit=100; downlimit=0&rft.coverage=uplimit=10; downlimit=0&rft.coverage=uplimit=10; downlimit=0&rft_rights=Copies of the publications, plus a list of accession numbers are available for download from the provided URL to AAD staff only.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547 when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA&rft_subject=ACAM&rft_subject=BACTERIA&rft_subject=MICROSCOPES&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=AMD&rft_subject=CEOS&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

Copies of the publications, plus a list of accession numbers are available for download from the provided URL to AAD staff only.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547 when using these data.

This metadata record is publicly available.

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Full description

Metadata record for data from ASAC Project 2547
See the link below for public details on this project.

Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this.

This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]).

The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.

Lineage

Progress Code: completed
Statement: The latitudes and longitudes provided in spatial coverage are taken from the metadata records for ASAC projects 708 and 1012.

Data time period: 2004-07-01 to 2005-07-31

This dataset is part of a larger collection

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79,-65 79,-67 76,-67 76,-65 79,-65

77.5,-66

80,-65 80,-68 70,-68 70,-65 80,-65

75,-66.5

text: westlimit=76; southlimit=-67; eastlimit=79; northlimit=-65

text: westlimit=70; southlimit=-68; eastlimit=80; northlimit=-65

text: uplimit=100; downlimit=0

text: uplimit=10; downlimit=0

Subjects
ACAM | AMD | AMD/AU | BACTERIA | CEOS | CONTINENT > ANTARCTICA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA | GEOGRAPHIC REGION > POLAR | LABORATORY | MICROSCOPES | biota | oceans |

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Other Information
Download point for the papers, plus a list of accession numbers - AAD Staff Only (GET DATA)

url : https://data.aad.gov.au/eds/2997/download

GenBank home page (GET DATA)

url : http://www.ncbi.nlm.nih.gov/genbank/

Public information for ASAC project 2547 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=2547

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2547

Identifiers
  • global : ASAC_2547
ACN 633 798 857