Data

Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)

Australian Antarctic Data Centre
FERRARI, BELINDA C ; RAY, ANGELIQUE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5e33a1e1e8c87&rft.title=Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)&rft.identifier=10.26179/5e33a1e1e8c87&rft.publisher=Australian Antarctic Data Centre&rft.description=Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL&rft.creator=FERRARI, BELINDA C &rft.creator=RAY, ANGELIQUE &rft.date=2020&rft.coverage=northlimit=-66.20709; southlimit=-66.38156; westlimit=110.42908; eastLimit=110.68176; projection=WGS84&rft.coverage=northlimit=-66.20709; southlimit=-66.38156; westlimit=110.42908; eastLimit=110.68176; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4406_BP_MP_2005 when using these data.&rft_subject=biota&rft_subject=MICROFAUNA&rft_subject=EARTH SCIENCE&rft_subject=LAND SURFACE&rft_subject=SOILS&rft_subject=MICROFLORA&rft_subject=METAGENOMIC&rft_subject=SOIL&rft_subject=MICROBIAL COMMUNITY ANALYSIS&rft_subject=SOIL MICROBIOME&rft_subject=ADS > Automated DNA Sequencer&rft_subject=FIELD SURVEYS&rft_subject=LABORATORY&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=CONTINENT > ANTARCTICA > MITCHELL PENINSULA&rft_subject=CONTINENT > ANTARCTICA > BROWNING PENINSULA&rft_subject=CONTINENT > ANTARCTICA > WINDMILL ISLANDS&rft_subject=CONTINENT > ANTARCTICA > EAST ANTARCTICA&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4406_BP_MP_2005 when using these data.

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Brief description

Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL

Issued: 2020-01-31

Data time period: 2005-01-01 to 2005-12-31

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110.68176,-66.20709 110.68176,-66.38156 110.42908,-66.38156 110.42908,-66.20709 110.68176,-66.20709

110.55542,-66.294325

text: northlimit=-66.20709; southlimit=-66.38156; westlimit=110.42908; eastLimit=110.68176; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | CONTINENT > ANTARCTICA > BROWNING PENINSULA | CONTINENT > ANTARCTICA > EAST ANTARCTICA | CONTINENT > ANTARCTICA > MITCHELL PENINSULA | CONTINENT > ANTARCTICA > WINDMILL ISLANDS | EARTH SCIENCE | FIELD SURVEYS | GEOGRAPHIC REGION > POLAR | LABORATORY | LAND SURFACE | METAGENOMIC | MICROBIAL COMMUNITY ANALYSIS | MICROFAUNA | MICROFLORA | SOIL | SOIL MICROBIOME | SOILS | biota |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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