Data

Material from PhD thesis: Appendix A-3 RPA primers and crRNA

University of Tasmania, Australia
Anuk Kruawan ; Andrew Flies
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://data.utas.edu.au/metadata/d6890a79-70cd-4bd5-8a34-72b4b6cb577e&rft.title=Material from PhD thesis: Appendix A-3 RPA primers and crRNA&rft.identifier=https://data.utas.edu.au/metadata/d6890a79-70cd-4bd5-8a34-72b4b6cb577e&rft.publisher=University of Tasmania, Australia&rft.description=This dataset contains supplemental material as part of a PhD thesis: Development of Novel Diagnostic Tools and Antigen Discovery for Devil Facial Tumour Disease. The material contains a list of recombinase polymerase amplification (RPA) primers and LwCas13a CRISPR RNA (crRNA) for the amplification and detection of DFT1, DFT2, RPL13a, and ZIKV. The material also includes a list of primer combinations. The best-performing primer pair and crRNA for each target are indicated. These primers and crRNA were used in the development of CRISPR-based point-of-care diagnostic tools, termed specific high-sensitivity enzymatic reporter unlocking (SHERLOCK), to identify DFT1 and DFT2 in the field with limited instrumentation.&rft.creator=Anuk Kruawan &rft.creator=Andrew Flies &rft.date=2024&rft.coverage=northlimit=-40.4051304422217; southlimit=-44.2247334454171; westlimit=143.749512657523; eastlimit=149.444825425744; projection=WGS84&rft_rights=Attribution(BY) http://creativecommons.org/licenses/by/4.0/&rft_subject=Animal immunology&rft_subject=Zoology&rft_subject=BIOLOGICAL SCIENCES&rft_subject=Infectious diseases&rft_subject=Clinical sciences&rft_subject=BIOMEDICAL AND CLINICAL SCIENCES&rft_subject=Veterinary immunology&rft_subject=Veterinary sciences&rft_subject=AGRICULTURAL, VETERINARY AND FOOD SCIENCES&rft_subject=Veterinary diagnostics&rft_subject=Veterinary pharmaceutical products&rft_subject=MANUFACTURING&rft_subject=Expanding knowledge in the biomedical and clinical sciences&rft_subject=Expanding knowledge&rft_subject=EXPANDING KNOWLEDGE&rft_subject=Expanding knowledge in the agricultural, food and veterinary sciences&rft_subject=diagnostic&rft_subject=cancer&rft_subject=crispr&rft_subject=Tasmanian devil&rft.type=dataset&rft.language=English Access the data
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This dataset contains supplemental material as part of a PhD thesis: Development of Novel Diagnostic Tools and Antigen Discovery for Devil Facial Tumour Disease. The material contains a list of recombinase polymerase amplification (RPA) primers and LwCas13a CRISPR RNA (crRNA) for the amplification and detection of DFT1, DFT2, RPL13a, and ZIKV. The material also includes a list of primer combinations. The best-performing primer pair and crRNA for each target are indicated. These primers and crRNA were used in the development of CRISPR-based point-of-care diagnostic tools, termed specific high-sensitivity enzymatic reporter unlocking (SHERLOCK), to identify DFT1 and DFT2 in the field with limited instrumentation.

Data time period: 2022 to 2024

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149.44483,-40.40513 149.44483,-44.22473 143.74951,-44.22473 143.74951,-40.40513 149.44483,-40.40513

146.59716904163,-42.31493194382

Subjects
Agricultural, Veterinary and Food Sciences | Animal Immunology | Biological Sciences | Biomedical and Clinical Sciences | Clinical Sciences | Expanding Knowledge | Expanding Knowledge | Expanding Knowledge in the Agricultural, Food and Veterinary Sciences | Expanding Knowledge in the Biomedical and Clinical Sciences | Infectious Diseases | Manufacturing | Tasmanian devil | Veterinary Diagnostics | Veterinary Immunology | Veterinary Pharmaceutical Products | Veterinary Sciences | Zoology | cancer | crispr | diagnostic |

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Identifiers
ACN 633 798 857